agar_with_e_coli_as_feed

CultureMech:002118 bacterial DEFINED SOLID_AGAR
Original name: AGAR WITH E. COLI AS FEED

Overview

External ontology
mediadive.medium:942 (source link unavailable) — AGAR WITH E. COLI AS FEED
Applications
  • Microbial cultivation
pH
7.2
Notes
DSMZ Medium 942 is a CaCl2-agar plate recipe overlaid with autoclaved Escherichia coli feed suspension lines/drops for myxobacteria; DSMZ specifies three separate drops of a thick ready-to-pipet suspension and does not fix an Escherichia coli concentration.

Composition

flowchart LR
    CultureMech_002118["agar_with_e_coli_as_feed"]
    ing0_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002118 --> ing0_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing0_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing1_Agar["Agar"]
    CultureMech_002118 --> ing1_Agar
    CHEBI_2509["CHEBI:2509"]
    ing1_Agar -.-> CHEBI_2509

Ingredients (2)

# Ingredient Concentration Ontology Notes
1 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1.0 G_PER_L CHEBI:86158
calcium chloride dihydrate
 DSMZ Medium 942 lists 1.0 g/L CaCl2 x 2 H2O.
2 Agar 15.0 G_PER_L CHEBI:2509
agar
 DSMZ Medium 942 lists 15.0 g/L Agar.

Preparation

  1. Mix 1.0 g/L CaCl2 x 2 H2O with 15.0 g/L agar to prepare the CaCl2-agar base.
  2. Adjust the CaCl2-agar base to pH 7.2.
  3. Autoclave the CaCl2-agar base.
  4. Pour the autoclaved CaCl2-agar base into plates.
  5. Grow Escherichia coli in liquid culture, collect the cells by centrifugation, and resuspend the pellet in enough water to produce a thick but ready-to-pipet feed suspension.
  6. Autoclave the Escherichia coli feed suspension separately.
  7. Place three separate feed-suspension drops on each CaCl2-agar plate, streak each drop once into an approximately 3 cm line, and inoculate the Escherichia coli lines/drops with myxobacteria.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.839313Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 942
2026-03-13T06:19:10.472501Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002118
2026-03-17T06:30:37.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:23.143439+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "AGAR WITH E. COLI AS FEED" to "agar_with_e_coli_as_feed" for consistent matching
2026-06-05T07:40:47.597470+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_dsmz_942_e_coli_feed_score15.py RESOLVED_DSMZ_942_E_COLI_FEED Verified DSMZ Medium 942 as a two-component CaCl2-agar plate recipe with an unquantified autoclaved Escherichia coli feed suspension, marked the simple composition as curated, added structured preparation and sterilization steps, and removed a stale KG-Microbe match to DSMZ Medium 49.