Overview
- External ontology
- mediadive.medium:993b (source link unavailable) — MODIFIED ISP5 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium993b.pdf
Composition
flowchart LR
CultureMech_002177["modified_isp5_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_002177 --> ing0_Yeast_extract
ing1_KCl["KCl"]
CultureMech_002177 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_L_Asparagine["L-Asparagine"]
CultureMech_002177 --> ing2_L_Asparagine
CHEBI_17196["CHEBI:17196"]
ing2_L_Asparagine -.-> CHEBI_17196
ing3_Glycerol["Glycerol"]
CultureMech_002177 --> ing3_Glycerol
CHEBI_17754["CHEBI:17754"]
ing3_Glycerol -.-> CHEBI_17754
ing4_K2HPO4["K2HPO4"]
CultureMech_002177 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_Agar["Agar"]
CultureMech_002177 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002177 --> ing6_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
ing7_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002177 --> ing7_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing7_MnCl2_x_4_H2O -.-> CHEBI_86368
ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002177 --> ing8_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 5 G_PER_L | ||
| 2 |
KCl
Cl.K 74.551 g/mol
|
100 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
L-Asparagine
C4H8N2O3 132.119 g/mol
|
1 G_PER_L |
CHEBI:17196
L-asparagine
|
|
| 4 |
Glycerol
C3H8O3 92.094 g/mol
|
10 G_PER_L |
CHEBI:17754
glycerol
|
|
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
|
| 7 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 8 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 9 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Preparation
- Prepare medium 993 supplemented with yeast extract (0.5 %) and KCl (10%). pH of the medium should be 7.2.
- The pH of trace salts solution is about 7.0-7.4. Do not adjust if it is within this range. Liquefy agar by steaming at 100°C for 15-20 minutes.
- Sterilize in flasks for pouring into Petri dishes. Seven standard 90 mm dishes containing 25 ml per dish.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_isp5_medium (CultureMech:002177)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.016547Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 993b |
| 2026-03-13T06:19:10.985308Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002177 |
| 2026-03-17T06:30:28.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.723613+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED ISP5 MEDIUM" to "modified_isp5_medium" for consistent matching |
| 2026-06-05T07:41:36.760863+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.673436+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:48.451297+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 4c49d8a24a23f92601e74b4ae85fa33712be3f8ecbef215db26be49d04bdb8ab |