modified_isp5_medium

CultureMech:002177 bacterial COMPLEX SOLID_AGAR
Original name: MODIFIED ISP5 MEDIUM

Overview

External ontology
mediadive.medium:993b (source link unavailable) — MODIFIED ISP5 MEDIUM
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium993b.pdf

Composition

flowchart LR
    CultureMech_002177["modified_isp5_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_002177 --> ing0_Yeast_extract
    ing1_KCl["KCl"]
    CultureMech_002177 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_L_Asparagine["L-Asparagine"]
    CultureMech_002177 --> ing2_L_Asparagine
    CHEBI_17196["CHEBI:17196"]
    ing2_L_Asparagine -.-> CHEBI_17196
    ing3_Glycerol["Glycerol"]
    CultureMech_002177 --> ing3_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing3_Glycerol -.-> CHEBI_17754
    ing4_K2HPO4["K2HPO4"]
    CultureMech_002177 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_Agar["Agar"]
    CultureMech_002177 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Yeast extract 5 G_PER_L
2 KCl
Cl.K 74.551 g/mol
100 G_PER_L CHEBI:32588
potassium chloride
3 L-Asparagine
C4H8N2O3 132.119 g/mol
1 G_PER_L CHEBI:17196
L-asparagine
4 Glycerol
C3H8O3 92.094 g/mol
10 G_PER_L CHEBI:17754
glycerol
5 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
6 Agar 20 G_PER_L CHEBI:2509
agar

Preparation

  1. Prepare medium 993 supplemented with yeast extract (0.5 %) and KCl (10%). pH of the medium should be 7.2.
  2. The pH of trace salts solution is about 7.0-7.4. Do not adjust if it is within this range. Liquefy agar by steaming at 100°C for 15-20 minutes.
  3. Sterilize in flasks for pouring into Petri dishes. Seven standard 90 mm dishes containing 25 ml per dish.

Solutions

Trace element solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeSO4 x 7 H2O 1 G_PER_L
MnCl2 x 4 H2O 1 G_PER_L
ZnSO4 x 7 H2O 1 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.016547Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 993b
2026-03-13T06:19:10.985308Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002177
2026-03-17T06:30:28.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:23.723613+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MODIFIED ISP5 MEDIUM" to "modified_isp5_medium" for consistent matching
2026-06-05T07:41:36.760863+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.673436+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T07:05:22.685195+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 3 stock-strength component(s) out of ingredients into 1 solution(s): 3 -> 'Trace element solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).