modified_isp5_medium_with_sodium_chloride

CultureMech:002178 bacterial DEFINED SOLID_AGAR
Original name: MODIFIED ISP5 MEDIUM WITH SODIUM CHLORIDE

Overview

External ontology
mediadive.medium:993c (source link unavailable) — MODIFIED ISP5 MEDIUM WITH SODIUM CHLORIDE
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium993c.pdf

Composition

flowchart LR
    CultureMech_002178["modified_isp5_medium_with_sodium_chloride"]
    ing0_NaCl["NaCl"]
    CultureMech_002178 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_L_Asparagine["L-Asparagine"]
    CultureMech_002178 --> ing1_L_Asparagine
    CHEBI_17196["CHEBI:17196"]
    ing1_L_Asparagine -.-> CHEBI_17196
    ing2_Glycerol["Glycerol"]
    CultureMech_002178 --> ing2_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing2_Glycerol -.-> CHEBI_17754
    ing3_K2HPO4["K2HPO4"]
    CultureMech_002178 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_Agar["Agar"]
    CultureMech_002178 --> ing4_Agar
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar -.-> CHEBI_2509
    ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002178 --> ing5_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing6_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002178 --> ing6_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing6_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002178 --> ing7_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
100 G_PER_L CHEBI:26710
sodium chloride
2 L-Asparagine
C4H8N2O3 132.119 g/mol
1 G_PER_L CHEBI:17196
L-asparagine
3 Glycerol
C3H8O3 92.094 g/mol
10 G_PER_L CHEBI:17754
glycerol
4 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
5 Agar 20 G_PER_L CHEBI:2509
agar
6 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
7 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
8 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate

Preparation

  1. Prepare medium 993 supplemented with NaCl (10 %). pH should be 7.2.
  2. Sterilize in flasks for pouring into Petri dishes. Seven standard 90 mm dishes containing 25 ml per dish.
  3. The pH of trace salts solution is about 7.0-7.4. Do not adjust if it is within this range. Liquefy agar by steaming at 100°C for 15-20 minutes.
  4. Sterilize in flasks for pouring into Petri dishes. Seven standard 90 mm dishes containing 25 ml per dish.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:13.018502Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 993c
2026-03-13T06:19:10.990785Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002178
2026-03-17T06:30:21.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:23.729576+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MODIFIED ISP5 MEDIUM WITH SODIUM CHLORIDE" to "modified_isp5_medium_with_sodium_chloride" for consistent matching
2026-06-05T07:41:36.768042+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.676061+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:48.459395+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 4580f48e7fa8f9f9c2a9ad1c98c0d5a9e00a55e42595db58c3ac5c30f1028449