modified_isp5_medium_with_sodium_chloride

CultureMech:002178 bacterial DEFINED SOLID_AGAR
Original name: MODIFIED ISP5 MEDIUM WITH SODIUM CHLORIDE

Overview

External ontology
mediadive.medium:993c (source link unavailable) — MODIFIED ISP5 MEDIUM WITH SODIUM CHLORIDE
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium993c.pdf

Composition

flowchart LR
    CultureMech_002178["modified_isp5_medium_with_sodium_chloride"]
    ing0_NaCl["NaCl"]
    CultureMech_002178 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_L_Asparagine["L-Asparagine"]
    CultureMech_002178 --> ing1_L_Asparagine
    CHEBI_17196["CHEBI:17196"]
    ing1_L_Asparagine -.-> CHEBI_17196
    ing2_Glycerol["Glycerol"]
    CultureMech_002178 --> ing2_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing2_Glycerol -.-> CHEBI_17754
    ing3_K2HPO4["K2HPO4"]
    CultureMech_002178 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_Agar["Agar"]
    CultureMech_002178 --> ing4_Agar
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar -.-> CHEBI_2509

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
100 G_PER_L CHEBI:26710
sodium chloride
2 L-Asparagine
C4H8N2O3 132.119 g/mol
1 G_PER_L CHEBI:17196
L-asparagine
3 Glycerol
C3H8O3 92.094 g/mol
10 G_PER_L CHEBI:17754
glycerol
4 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
5 Agar 20 G_PER_L CHEBI:2509
agar

Preparation

  1. Prepare medium 993 supplemented with NaCl (10 %). pH should be 7.2.
  2. Sterilize in flasks for pouring into Petri dishes. Seven standard 90 mm dishes containing 25 ml per dish.
  3. The pH of trace salts solution is about 7.0-7.4. Do not adjust if it is within this range. Liquefy agar by steaming at 100°C for 15-20 minutes.
  4. Sterilize in flasks for pouring into Petri dishes. Seven standard 90 mm dishes containing 25 ml per dish.

Solutions

Trace element solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeSO4 x 7 H2O 1 G_PER_L
MnCl2 x 4 H2O 1 G_PER_L
ZnSO4 x 7 H2O 1 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.018502Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 993c
2026-03-13T06:19:10.990785Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002178
2026-03-17T06:30:21.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:23.729576+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MODIFIED ISP5 MEDIUM WITH SODIUM CHLORIDE" to "modified_isp5_medium_with_sodium_chloride" for consistent matching
2026-06-05T07:41:36.768042+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.676061+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T07:05:22.693096+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 3 stock-strength component(s) out of ingredients into 1 solution(s): 3 -> 'Trace element solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).