rumen_bacteria_medium

Overview

External ontology

mediadive.medium:J1029 (source link unavailable) — RUMEN BACTERIA MEDIUM

Applications

• Microbial cultivation

pH

6.8

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1029

Composition

flowchart LR
    CultureMech_002212["rumen_bacteria_medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_002212 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_Trypticase_peptone["Trypticase peptone"]
    CultureMech_002212 --> ing1_Trypticase_peptone
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_002212 --> ing2_Yeast_extract
    ing3_Hemin["Hemin"]
    CultureMech_002212 --> ing3_Hemin
    CHEBI_50385["CHEBI:50385"]
    ing3_Hemin -.-> CHEBI_50385
    ing4_Glucose["Glucose"]
    CultureMech_002212 --> ing4_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing4_Glucose -.-> CHEBI_17234
    ing5_Maltose["Maltose"]
    CultureMech_002212 --> ing5_Maltose
    CHEBI_17306["CHEBI:17306"]
    ing5_Maltose -.-> CHEBI_17306
    ing6_Cellobiose["Cellobiose"]
    CultureMech_002212 --> ing6_Cellobiose
    CHEBI_17057["CHEBI:17057"]
    ing6_Cellobiose -.-> CHEBI_17057
    ing7_Starch["Starch"]
    CultureMech_002212 --> ing7_Starch
    CHEBI_28017["CHEBI:28017"]
    ing7_Starch -.-> CHEBI_28017
    ing8_Glycerol["Glycerol"]
    CultureMech_002212 --> ing8_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing8_Glycerol -.-> CHEBI_17754
    ing9_Na2CO3["Na2CO3"]
    CultureMech_002212 --> ing9_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing9_Na2CO3 -.-> CHEBI_29377
    ing10_Resazurin["Resazurin"]
    CultureMech_002212 --> ing10_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Resazurin -.-> CHEBI_8806
    ing11_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002212 --> ing11_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing11_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002212 --> ing12_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing12_Na2S_x_9_H2O -.-> CHEBI_76209
    ing13_KH2PO4["KH2PO4"]
    CultureMech_002212 --> ing13_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing13_KH2PO4 -.-> CHEBI_63036
    ing14_NaCl["NaCl"]
    CultureMech_002212 --> ing14_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing14_NaCl -.-> CHEBI_26710
    ing15__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002212 --> ing15__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing15__NH4_2SO4 -.-> CHEBI_62946
    ing16_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002212 --> ing16_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing16_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing17_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002212 --> ing17_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing17_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing18_Acetic_acid["Acetic acid"]
    CultureMech_002212 --> ing18_Acetic_acid
    CHEBI_15366["CHEBI:15366"]
    ing18_Acetic_acid -.-> CHEBI_15366
    ing19_Propionic_acid["Propionic acid"]
    CultureMech_002212 --> ing19_Propionic_acid
    CHEBI_30768["CHEBI:30768"]
    ing19_Propionic_acid -.-> CHEBI_30768
    ing20_n_Butyric_acid["n-Butyric acid"]
    CultureMech_002212 --> ing20_n_Butyric_acid
    CHEBI_30772["CHEBI:30772"]
    ing20_n_Butyric_acid -.-> CHEBI_30772
    ing21_iso_Butyric_acid["iso-Butyric acid"]
    CultureMech_002212 --> ing21_iso_Butyric_acid
    CHEBI_16135["CHEBI:16135"]
    ing21_iso_Butyric_acid -.-> CHEBI_16135
    ing22_n_Valeric_acid["n-Valeric acid"]
    CultureMech_002212 --> ing22_n_Valeric_acid
    CHEBI_17418["CHEBI:17418"]
    ing22_n_Valeric_acid -.-> CHEBI_17418
    ing23_iso_Valeric_acid["iso-Valeric acid"]
    CultureMech_002212 --> ing23_iso_Valeric_acid
    CHEBI_28484["CHEBI:28484"]
    ing23_iso_Valeric_acid -.-> CHEBI_28484
    ing24_DL_alpha_Methyl_butyric_acid["DL-alpha-Methyl butyric acid"]
    CultureMech_002212 --> ing24_DL_alpha_Methyl_butyric_acid
    CHEBI_37070["CHEBI:37070"]
    ing24_DL_alpha_Methyl_butyric_acid -.-> CHEBI_37070

Ingredients (25)

Preparation

1. Adjust pH to 6.7 - 6.8; gas atmosphere: 100% CO2.
2. Dissolve ingredients except carbohydrates, Na2CO3, cysteine and sodium sulfide in distilled water, boil for 5 min, then cool to room temperature while gassing with 100% CO2. Add Na2CO3 and equilibrate the medium with the gas to pH 6.8. Distribute under CO2 in rubber-stoppered tubes (i.e. Hungate tubes) and autoclave. Thereafter add carbohydrate mixture, cysteine and sodium sulfide from concentrated sterile stock solutions (prepared anaerobically under N2 gas and autoclaved).

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• JCM_J1029_RUMEN_BACTERIA_MEDIUM (CultureMech:002212)