Overview
- External ontology
- mediadive.medium:J1029 (source link unavailable) — RUMEN BACTERIA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1029 Original JCM1029 is unavailable: its current endpoint returned no recipe. This formulation is supported by the inspected MediaDive dataset only. Its declared volume and g_l fields do not establish the original final-volume basis. Main and mineral masses are retained as exact batch inputs in notes; acid inputs retain liquid volumes. Existing preparation descriptions are dataset assertions, not newly verified original methods. Complete inspected source rows define this reconstruction. Archive old flattened quantities, false LB expansions, placeholders and copied stock-solvent claims in append-only history. Unrelated annotations remain preserved, not recertified.
Composition
flowchart LR
CultureMech_002212["rumen_bacteria_medium"]
ing0_K2HPO4["K2HPO4"]
CultureMech_002212 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_Trypticase_peptone["Trypticase peptone"]
CultureMech_002212 --> ing1_Trypticase_peptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_002212 --> ing2_Yeast_extract
ing3_Hemin["Hemin"]
CultureMech_002212 --> ing3_Hemin
CHEBI_50385["CHEBI:50385"]
ing3_Hemin -.-> CHEBI_50385
ing4_Glucose["Glucose"]
CultureMech_002212 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_Maltose["Maltose"]
CultureMech_002212 --> ing5_Maltose
CHEBI_17306["CHEBI:17306"]
ing5_Maltose -.-> CHEBI_17306
ing6_Cellobiose["Cellobiose"]
CultureMech_002212 --> ing6_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing6_Cellobiose -.-> CHEBI_17057
ing7_Starch["Starch"]
CultureMech_002212 --> ing7_Starch
CHEBI_28017["CHEBI:28017"]
ing7_Starch -.-> CHEBI_28017
ing8_Glycerol["Glycerol"]
CultureMech_002212 --> ing8_Glycerol
CHEBI_17754["CHEBI:17754"]
ing8_Glycerol -.-> CHEBI_17754
ing9_Na2CO3["Na2CO3"]
CultureMech_002212 --> ing9_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing9_Na2CO3 -.-> CHEBI_29377
ing10_Resazurin["Resazurin"]
CultureMech_002212 --> ing10_Resazurin
CHEBI_8806["CHEBI:8806"]
ing10_Resazurin -.-> CHEBI_8806
ing11_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002212 --> ing11_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing11_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002212 --> ing12_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing12_Na2S_x_9_H2O -.-> CHEBI_76209
ing13_Distilled_water["Distilled water"]
CultureMech_002212 --> ing13_Distilled_water
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
CHEBI:131527
dipotassium hydrogen phosphate
|
Keep literal source mass and preparation stage in evidenced notes; no assumed receiving-medium denominator; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 0.3 g; stage=Main sol. J1029. Dataset g_l=0.2997 is retained here as provider context, not original-methods concentration evidence. | |
| 2 | Trypticase peptone | Keep literal source mass and preparation stage in evidenced notes; no assumed receiving-medium denominator; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 2 g; stage=Main sol. J1029. Source attribute: BD-BBL. Dataset g_l=1.998 is retained here as provider context, not original-methods concentration evidence. | ||
| 3 | Yeast extract | Keep literal source mass and preparation stage in evidenced notes; no assumed receiving-medium denominator; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 0.5 g; stage=Main sol. J1029. Dataset g_l=0.499501 is retained here as provider context, not original-methods concentration evidence. | ||
| 4 |
Hemin
C34H32ClFeN4O4 651.952 g/mol
|
CHEBI:50385
hemin
|
Keep literal source mass and preparation stage in evidenced notes; no assumed receiving-medium denominator; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 1 mg; stage=Main sol. J1029. Dataset g_l=0.000999001 is retained here as provider context, not original-methods concentration evidence. | |
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
CHEBI:17234
glucose
|
Keep literal source mass and preparation stage in evidenced notes; no assumed receiving-medium denominator; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 0.5 g; stage=Main sol. J1029. Dataset g_l=0.499501 is retained here as provider context, not original-methods concentration evidence. | |
| 6 |
Maltose
C12H22O11 342.297 g/mol
|
CHEBI:17306
maltose
|
Keep literal source mass and preparation stage in evidenced notes; no assumed receiving-medium denominator; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 0.5 g; stage=Main sol. J1029. Dataset g_l=0.499501 is retained here as provider context, not original-methods concentration evidence. | |
| 7 |
Cellobiose
C12H22O11 342.297 g/mol
|
CHEBI:17057
cellobiose
|
Keep literal source mass and preparation stage in evidenced notes; no assumed receiving-medium denominator; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 0.5 g; stage=Main sol. J1029. Dataset g_l=0.499501 is retained here as provider context, not original-methods concentration evidence. | |
| 8 | Starch |
CHEBI:28017
starch
|
( soluble) Keep literal source mass and preparation stage in evidenced notes; no assumed receiving-medium denominator; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 0.5 g; stage=Main sol. J1029. Dataset g_l=0.499501 is retained here as provider context, not original-methods concentration evidence. | |
| 9 |
Glycerol
C3H8O3 92.094 g/mol
|
CHEBI:17754
glycerol
|
Keep literal source mass and preparation stage in evidenced notes; no assumed receiving-medium denominator; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 0.5 g; stage=Main sol. J1029. Dataset g_l=0.499501 is retained here as provider context, not original-methods concentration evidence. | |
| 10 |
Na2CO3
CO3.2Na 105.988 g/mol
|
CHEBI:29377
sodium carbonate
|
Keep literal source mass and preparation stage in evidenced notes; no assumed receiving-medium denominator; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 4 g; stage=Main sol. J1029. Dataset g_l=3.996 is retained here as provider context, not original-methods concentration evidence. | |
| 11 |
Resazurin
C12H7NO4 229.191 g/mol
|
CHEBI:8806
Resazurin
|
Keep literal source mass and preparation stage in evidenced notes; no assumed receiving-medium denominator; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 1 mg; stage=Main sol. J1029. Dataset g_l=0.000999001 is retained here as provider context, not original-methods concentration evidence. | |
| 12 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Keep literal source mass and preparation stage in evidenced notes; no assumed receiving-medium denominator; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 0.25 g; stage=Main sol. J1029. Dataset g_l=0.24975 is retained here as provider context, not original-methods concentration evidence. | |
| 13 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
CHEBI:76209
sodium sulfide nonahydrate
|
Keep literal source mass and preparation stage in evidenced notes; no assumed receiving-medium denominator; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 0.25 g; stage=Main sol. J1029. Dataset g_l=0.24975 is retained here as provider context, not original-methods concentration evidence. | |
| 14 | Distilled water | 0.96 L | Source input only; not final volume or volume percentage; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 960 ml; stage=Main sol. J1029. |
Preparation
- Adjust pH to 6.7 - 6.8; gas atmosphere: 100% CO2.
- Dissolve ingredients except carbohydrates, Na2CO3, cysteine and sodium sulfide in distilled water, boil for 5 min, then cool to room temperature while gassing with 100% CO2. Add Na2CO3 and equilibrate the medium with the gas to pH 6.8. Distribute under CO2 in rubber-stoppered tubes (i.e. Hungate tubes) and autoclave. Thereafter add carbohydrate mixture, cysteine and sodium sulfide from concentrated sterile stock solutions (prepared anaerobically under N2 gas and autoclaved).
Solutions
Mineral solution
Dose: 0.038 L
| Ingredient | Concentration | Notes |
|---|---|---|
| KH2PO4 | Keep literal source mass and preparation stage in evidenced notes; no assumed receiving-medium denominator; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 6 g; stage=Mineral solution. Dataset g_l=6 is retained here as provider context, not original-methods concentration evidence. | |
| NaCl | Keep literal source mass and preparation stage in evidenced notes; no assumed receiving-medium denominator; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 12 g; stage=Mineral solution. Dataset g_l=12 is retained here as provider context, not original-methods concentration evidence. | |
| (NH4)2SO4 | Keep literal source mass and preparation stage in evidenced notes; no assumed receiving-medium denominator; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 6 g; stage=Mineral solution. Dataset g_l=6 is retained here as provider context, not original-methods concentration evidence. | |
| CaCl2 x 2 H2O | Keep literal source mass and preparation stage in evidenced notes; no assumed receiving-medium denominator; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 1.6 g; stage=Mineral solution. Dataset g_l=1.6 is retained here as provider context, not original-methods concentration evidence. | |
| MgSO4 x 7 H2O | Keep literal source mass and preparation stage in evidenced notes; no assumed receiving-medium denominator; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 2.5 g; stage=Mineral solution. Dataset g_l=2.5 is retained here as provider context, not original-methods concentration evidence. | |
| Distilled water | 1 L | Source input only; not final volume or volume percentage; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 1000 ml; stage=Mineral solution. |
Source input only; not final volume or volume percentage; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 38 ml; stage=Main sol. J1029.
VFA solution
Dose: 0.0031 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Acetic acid | 0.017 L | Source input only; not final volume or volume percentage; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 17 ml; stage=VFA solution. |
| Propionic acid | 0.006 L | Source input only; not final volume or volume percentage; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 6 ml; stage=VFA solution. |
| n-Butyric acid | 0.004 L | Source input only; not final volume or volume percentage; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 4 ml; stage=VFA solution. |
| iso-Butyric acid | 0.001 L | Source input only; not final volume or volume percentage; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 1 ml; stage=VFA solution. |
| n-Valeric acid | 0.001 L | Source input only; not final volume or volume percentage; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 1 ml; stage=VFA solution. |
| iso-Valeric acid | 0.001 L | Source input only; not final volume or volume percentage; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 1 ml; stage=VFA solution. |
| DL-alpha-Methyl butyric acid | 0.001 L | Source input only; not final volume or volume percentage; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 1 ml; stage=VFA solution. |
Source input only; not final volume or volume percentage; original JCM1029 endpoint returned no recipe in the live fetch. This inspected source is the MediaDive dataset; original final volume remains unverified. Preserve reported g_l as explicitly qualified source context, not an original-methods claim. Source input: 3.1 ml; stage=Main sol. J1029.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-26T17:53:18.369897Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1029 |
| 2026-03-13T06:19:11.286870Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002212 |
| 2026-03-17T06:30:25.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:24.062647+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RUMEN BACTERIA MEDIUM" to "rumen_bacteria_medium" for consistent matching |
| 2026-06-05T07:40:16.459698+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.710022+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-09T08:40:02.418657+00:00 | codex | RESTORED_RUMEN_BACTERIA_SOURCE_FORMULATIONS_WITH_EVIDENCE | Restore source-specific medium330 ingredients, stock scopes and input units with literal evidence. Preserve prior supported entries, provider nulls, unknown original bases and the JCM1029 access gap. No growth association added. |