Overview
- External ontology
- mediadive.medium:J1037 (source link unavailable) — MPYCY AGAR MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.7
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1037
Composition
flowchart LR
CultureMech_002221["mpycy_agar_medium"]
ing0_Maltose_monohydrate["Maltose monohydrate"]
CultureMech_002221 --> ing0_Maltose_monohydrate
ing1_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_002221 --> ing1_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing1_Sodium_pyruvate -.-> CHEBI_50144
ing2_Casamino_acids["Casamino acids"]
CultureMech_002221 --> ing2_Casamino_acids
ing3_Yeast_extract["Yeast extract"]
CultureMech_002221 --> ing3_Yeast_extract
ing4_Agar["Agar"]
CultureMech_002221 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
ing5__NH4_2SO4["(NH4)2SO4"]
CultureMech_002221 --> ing5__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing5__NH4_2SO4 -.-> CHEBI_62946
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002221 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_KCl["KCl"]
CultureMech_002221 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002221 --> ing8_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
ing9_Na2HPO4_NaH2PO4_buffer["Na2HPO4-NaH2PO4 buffer"]
CultureMech_002221 --> ing9_Na2HPO4_NaH2PO4_buffer
ing10_Riboflavin["Riboflavin"]
CultureMech_002221 --> ing10_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing10_Riboflavin -.-> CHEBI_17015
ing11_Thiamine_HCl["Thiamine HCl"]
CultureMech_002221 --> ing11_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing11_Thiamine_HCl -.-> CHEBI_49105
ing12_Nicotinic_acid["Nicotinic acid"]
CultureMech_002221 --> ing12_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing12_Nicotinic_acid -.-> CHEBI_15940
ing13_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002221 --> ing13_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing13_Calcium_pantothenate -.-> CHEBI_31345
ing14_Myo_inositol["Myo-inositol"]
CultureMech_002221 --> ing14_Myo_inositol
CHEBI_17268["CHEBI:17268"]
ing14_Myo_inositol -.-> CHEBI_17268
ing15_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002221 --> ing15_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing15_p_Aminobenzoic_acid -.-> CHEBI_30753
ing16_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002221 --> ing16_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing16_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing17_Folic_acid["Folic acid"]
CultureMech_002221 --> ing17_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing17_Folic_acid -.-> CHEBI_27470
ing18_Vitamin_B12["Vitamin B12"]
CultureMech_002221 --> ing18_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing18_Vitamin_B12 -.-> CHEBI_176843
ing19_Biotin["Biotin"]
CultureMech_002221 --> ing19_Biotin
CHEBI_15956["CHEBI:15956"]
ing19_Biotin -.-> CHEBI_15956
ing20_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_002221 --> ing20_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing20_FeCl3_x_6_H2O -.-> CHEBI_86254
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Maltose monohydrate | 0.493583 G_PER_L | ||
| 2 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.394867 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 3 | Casamino acids | 0.29615 G_PER_L | ||
| 4 | Yeast extract | 0.29615 G_PER_L | ||
| 5 | Agar | 11.846 G_PER_L |
CHEBI:2509
agar
|
|
| 6 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
20 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
10 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 8 |
KCl
Cl.K 74.551 g/mol
|
10 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 9 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
4 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 10 | Na2HPO4-NaH2PO4 buffer | 2 G_PER_L | ||
| 11 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.02 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 12 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.02 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 13 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.02 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 14 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.02 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 15 |
Myo-inositol
C6H12O6 180.156 g/mol
|
0.02 G_PER_L |
CHEBI:17268
myo-inositol
|
|
| 16 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.02 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 17 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.02 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 18 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.001 G_PER_L |
CHEBI:27470
folic acid
|
|
| 19 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 20 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.001 G_PER_L |
CHEBI:15956
biotin
|
|
| 21 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.5 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Preparation
- Before autoclaving, adjust pH to 7.6 - 7.8 for agar plate and to 7.4 - 7.6 for liquid medium. Autoclave at 115C for 20 min. Separately sterilize Solution B by filtration and add to the medium aseptically.
- Mix and filter-sterilize just before addition to the medium.
- Prepare just before use.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mpycy_agar_medium (CultureMech:002221)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.406099Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1037 |
| 2026-01-28T19:48:14.272262Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:11.393257Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002221 |
| 2026-03-17T06:29:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:24.181252+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MPYCY AGAR MEDIUM" to "mpycy_agar_medium" for consistent matching |
| 2026-06-05T07:41:38.338699+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:49.243659+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:47:09.140397+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:50.312924+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 650fe64d4bd454c5cb60ace6103433cbc32e256aaab5f50b2ba26bb623113cad |