mpycy_agar_medium

CultureMech:002221 bacterial COMPLEX SOLID_AGAR
Original name: MPYCY AGAR MEDIUM

Overview

External ontology
mediadive.medium:J1037 (source link unavailable) — MPYCY AGAR MEDIUM
Applications
  • Microbial cultivation
pH
7.7
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1037

Composition

flowchart LR
    CultureMech_002221["mpycy_agar_medium"]
    ing0_Maltose_monohydrate["Maltose monohydrate"]
    CultureMech_002221 --> ing0_Maltose_monohydrate
    ing1_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_002221 --> ing1_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing1_Sodium_pyruvate -.-> CHEBI_50144
    ing2_Casamino_acids["Casamino acids"]
    CultureMech_002221 --> ing2_Casamino_acids
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_002221 --> ing3_Yeast_extract
    ing4_Agar["Agar"]
    CultureMech_002221 --> ing4_Agar
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar -.-> CHEBI_2509
    ing5__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002221 --> ing5__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing5__NH4_2SO4 -.-> CHEBI_62946
    ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002221 --> ing6_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing7_KCl["KCl"]
    CultureMech_002221 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002221 --> ing8_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing9_Na2HPO4_NaH2PO4_buffer["Na2HPO4-NaH2PO4 buffer"]
    CultureMech_002221 --> ing9_Na2HPO4_NaH2PO4_buffer
    ing10_Riboflavin["Riboflavin"]
    CultureMech_002221 --> ing10_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing10_Riboflavin -.-> CHEBI_17015
    ing11_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002221 --> ing11_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing11_Thiamine_HCl -.-> CHEBI_49105
    ing12_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002221 --> ing12_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing12_Nicotinic_acid -.-> CHEBI_15940
    ing13_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002221 --> ing13_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing13_Calcium_pantothenate -.-> CHEBI_31345
    ing14_Myo_inositol["Myo-inositol"]
    CultureMech_002221 --> ing14_Myo_inositol
    CHEBI_17268["CHEBI:17268"]
    ing14_Myo_inositol -.-> CHEBI_17268
    ing15_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002221 --> ing15_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing15_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing16_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002221 --> ing16_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing16_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing17_Folic_acid["Folic acid"]
    CultureMech_002221 --> ing17_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing17_Folic_acid -.-> CHEBI_27470
    ing18_Vitamin_B12["Vitamin B12"]
    CultureMech_002221 --> ing18_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing18_Vitamin_B12 -.-> CHEBI_176843
    ing19_Biotin["Biotin"]
    CultureMech_002221 --> ing19_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing19_Biotin -.-> CHEBI_15956
    ing20_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_002221 --> ing20_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing20_FeCl3_x_6_H2O -.-> CHEBI_86254

Ingredients (21)

# Ingredient Concentration Ontology Notes
1 Maltose monohydrate 0.493583 G_PER_L
2 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
0.394867 G_PER_L CHEBI:50144
sodium pyruvate
3 Casamino acids 0.29615 G_PER_L
4 Yeast extract 0.29615 G_PER_L
5 Agar 11.846 G_PER_L CHEBI:2509
agar
6 (NH4)2SO4
2H4N.O4S 132.141 g/mol
20 G_PER_L CHEBI:62946
ammonium sulfate
7 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
10 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
8 KCl
Cl.K 74.551 g/mol
10 G_PER_L CHEBI:32588
potassium chloride
9 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
4 G_PER_L CHEBI:86158
calcium chloride dihydrate
10 Na2HPO4-NaH2PO4 buffer 2 G_PER_L
11 Riboflavin
C17H20N4O6 376.369 g/mol
0.02 G_PER_L CHEBI:17015
riboflavin
12 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.02 G_PER_L CHEBI:49105
thiamine hydrochloride
13 Nicotinic acid
C6H5NO2 123.111 g/mol
0.02 G_PER_L CHEBI:15940
nicotinic acid
14 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.02 G_PER_L CHEBI:31345
Calcium pantothenate
15 Myo-inositol
C6H12O6 180.156 g/mol
0.02 G_PER_L CHEBI:17268
myo-inositol
16 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.02 G_PER_L CHEBI:30753
4-aminobenzoic acid
17 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.02 G_PER_L CHEBI:30961
pyridoxine hydrochloride
18 Folic acid
C19H19N7O6 441.404 g/mol
0.001 G_PER_L CHEBI:27470
folic acid
19 Vitamin B12 0.001 G_PER_L CHEBI:176843
vitamin B12
20 Biotin
C10H16N2O3S 244.316 g/mol
0.001 G_PER_L CHEBI:15956
biotin
21 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
0.5 G_PER_L CHEBI:86254
iron trichloride hexahydrate

Preparation

  1. Before autoclaving, adjust pH to 7.6 - 7.8 for agar plate and to 7.4 - 7.6 for liquid medium. Autoclave at 115C for 20 min. Separately sterilize Solution B by filtration and add to the medium aseptically.
  2. Mix and filter-sterilize just before addition to the medium.
  3. Prepare just before use.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.406099Z mediadive-import Imported from MediaDive Source: JCM, ID: J1037
2026-01-28T19:48:14.272262Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:11.393257Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002221
2026-03-17T06:29:55.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:24.181252+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MPYCY AGAR MEDIUM" to "mpycy_agar_medium" for consistent matching
2026-06-05T07:41:38.338699+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:49.243659+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:47:09.140397+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.