Overview
- External ontology
- mediadive.medium:J1042 (source link unavailable) — CALROANAEROBACTER MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1042
Composition
flowchart LR
CultureMech_002227["calroanaerobacter_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_002227 --> ing0_Yeast_extract
ing1_Tryptone["Tryptone"]
CultureMech_002227 --> ing1_Tryptone
ing2_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_002227 --> ing2_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing2_Sodium_pyruvate -.-> CHEBI_50144
ing3_PIPES["PIPES"]
CultureMech_002227 --> ing3_PIPES
CHEBI_39033["CHEBI:39033"]
ing3_PIPES -.-> CHEBI_39033
ing4_NaCl["NaCl"]
CultureMech_002227 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002227 --> ing5_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002227 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7__NH4_2SO4["(NH4)2SO4"]
CultureMech_002227 --> ing7__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing7__NH4_2SO4 -.-> CHEBI_62946
ing8_NaHCO3["NaHCO3"]
CultureMech_002227 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002227 --> ing9_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing9_CaCl2_x_2_H2O -.-> CHEBI_86158
ing10_KCl["KCl"]
CultureMech_002227 --> ing10_KCl
CHEBI_32588["CHEBI:32588"]
ing10_KCl -.-> CHEBI_32588
ing11_KH2PO4["KH2PO4"]
CultureMech_002227 --> ing11_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing11_KH2PO4 -.-> CHEBI_63036
ing12_NaBr["NaBr"]
CultureMech_002227 --> ing12_NaBr
CHEBI_63004["CHEBI:63004"]
ing12_NaBr -.-> CHEBI_63004
ing13_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_002227 --> ing13_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing13_SrCl2_x_6_H2O -.-> CHEBI_36385
ing14_Ammonium_ferric_citrate["Ammonium ferric citrate"]
CultureMech_002227 --> ing14_Ammonium_ferric_citrate
CHEBI_31604["CHEBI:31604"]
ing14_Ammonium_ferric_citrate -.-> CHEBI_31604
ing15_Biotin["Biotin"]
CultureMech_002227 --> ing15_Biotin
CHEBI_15956["CHEBI:15956"]
ing15_Biotin -.-> CHEBI_15956
ing16_Folic_acid["Folic acid"]
CultureMech_002227 --> ing16_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing16_Folic_acid -.-> CHEBI_27470
ing17_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002227 --> ing17_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing17_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing18_Thiamine_HCl["Thiamine HCl"]
CultureMech_002227 --> ing18_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing18_Thiamine_HCl -.-> CHEBI_49105
ing19_Riboflavin["Riboflavin"]
CultureMech_002227 --> ing19_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing19_Riboflavin -.-> CHEBI_17015
ing20_Nicotinic_acid["Nicotinic acid"]
CultureMech_002227 --> ing20_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing20_Nicotinic_acid -.-> CHEBI_15940
ing21_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002227 --> ing21_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing21_Calcium_pantothenate -.-> CHEBI_31345
ing22_Vitamin_B12["Vitamin B12"]
CultureMech_002227 --> ing22_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing22_Vitamin_B12 -.-> CHEBI_176843
ing23_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002227 --> ing23_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing23_p_Aminobenzoic_acid -.-> CHEBI_30753
ing24_Lipoic_acid["Lipoic acid"]
CultureMech_002227 --> ing24_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing24_Lipoic_acid -.-> CHEBI_16494
ing25_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002227 --> ing25_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing25_Nitrilotriacetic_acid -.-> CHEBI_44557
ing26_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002227 --> ing26_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing26_MnSO4_x_n_H2O -.-> CHEBI_86360
ing27_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002227 --> ing27_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing27_FeSO4_x_7_H2O -.-> CHEBI_75836
ing28_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002227 --> ing28_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing28_CoSO4_x_7_H2O -.-> CHEBI_91244
ing29_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002227 --> ing29_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing29_ZnSO4_x_7_H2O -.-> CHEBI_32312
CultureMech_002227 --> more["...4 more"]
Ingredients (34)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 1.99005 G_PER_L | ||
| 2 | Tryptone | 1.99005 G_PER_L | ||
| 3 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
4.97512 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 4 | PIPES | 3.44279 G_PER_L |
CHEBI:39033
PIPES
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
22.8905 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 21.8905, 1.0] |
| 6 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.98507 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
8.97015 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 5.97015, 3.0] |
| 8 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.995025 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.199005 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 10 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.39850700000000006 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.298507, 0.1] |
| 11 |
KCl
Cl.K 74.551 g/mol
|
0.497512 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 12 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.41791 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 13 |
NaBr
Br.Na 102.894 g/mol
|
0.0497512 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 14 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.0199005 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 15 | Ammonium ferric citrate | 0.00995025 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
|
| 16 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 17 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 18 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 19 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 20 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 21 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 22 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 23 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 24 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 25 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 26 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 27 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 28 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 29 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 30 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 31 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 32 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 33 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 34 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Adjust pH to 7.0. Distribute the medium into culture vessels under a N2 gas atmosphere, seal with butyl rubber stoppers and autoclave.
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- calroanaerobacter_medium (CultureMech:002227)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.421345Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1042 |
| 2026-03-13T03:09:17.217471Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:11.439235Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002227 |
| 2026-03-17T06:30:25.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:24.231132+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CALROANAEROBACTER MEDIUM" to "calroanaerobacter_medium" for consistent matching |
| 2026-06-05T07:40:52.411570+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.266937+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:26.620398+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 375948baaccb49933f73ea6a888095e7e5b31fdbfd1b367a43b58579ab39978e |