Overview
- External ontology
- mediadive.medium:J1042 (source link unavailable) — CALROANAEROBACTER MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1042
Composition
flowchart LR
CultureMech_002227["calroanaerobacter_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_002227 --> ing0_Yeast_extract
ing1_Tryptone["Tryptone"]
CultureMech_002227 --> ing1_Tryptone
ing2_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_002227 --> ing2_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing2_Sodium_pyruvate -.-> CHEBI_50144
ing3_PIPES["PIPES"]
CultureMech_002227 --> ing3_PIPES
CHEBI_39033["CHEBI:39033"]
ing3_PIPES -.-> CHEBI_39033
ing4_NaCl["NaCl"]
CultureMech_002227 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002227 --> ing5_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002227 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7__NH4_2SO4["(NH4)2SO4"]
CultureMech_002227 --> ing7__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing7__NH4_2SO4 -.-> CHEBI_62946
ing8_NaHCO3["NaHCO3"]
CultureMech_002227 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002227 --> ing9_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing9_CaCl2_x_2_H2O -.-> CHEBI_86158
ing10_KCl["KCl"]
CultureMech_002227 --> ing10_KCl
CHEBI_32588["CHEBI:32588"]
ing10_KCl -.-> CHEBI_32588
ing11_KH2PO4["KH2PO4"]
CultureMech_002227 --> ing11_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing11_KH2PO4 -.-> CHEBI_63036
ing12_NaBr["NaBr"]
CultureMech_002227 --> ing12_NaBr
CHEBI_63004["CHEBI:63004"]
ing12_NaBr -.-> CHEBI_63004
ing13_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_002227 --> ing13_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing13_SrCl2_x_6_H2O -.-> CHEBI_36385
ing14_Ammonium_ferric_citrate["Ammonium ferric citrate"]
CultureMech_002227 --> ing14_Ammonium_ferric_citrate
CHEBI_31604["CHEBI:31604"]
ing14_Ammonium_ferric_citrate -.-> CHEBI_31604
ing15_Biotin["Biotin"]
CultureMech_002227 --> ing15_Biotin
CHEBI_15956["CHEBI:15956"]
ing15_Biotin -.-> CHEBI_15956
ing16_Folic_acid["Folic acid"]
CultureMech_002227 --> ing16_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing16_Folic_acid -.-> CHEBI_27470
ing17_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002227 --> ing17_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing17_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing18_Thiamine_HCl["Thiamine HCl"]
CultureMech_002227 --> ing18_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing18_Thiamine_HCl -.-> CHEBI_49105
ing19_Riboflavin["Riboflavin"]
CultureMech_002227 --> ing19_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing19_Riboflavin -.-> CHEBI_17015
ing20_Nicotinic_acid["Nicotinic acid"]
CultureMech_002227 --> ing20_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing20_Nicotinic_acid -.-> CHEBI_15940
ing21_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002227 --> ing21_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing21_Calcium_pantothenate -.-> CHEBI_31345
ing22_Vitamin_B12["Vitamin B12"]
CultureMech_002227 --> ing22_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing22_Vitamin_B12 -.-> CHEBI_176843
ing23_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002227 --> ing23_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing23_p_Aminobenzoic_acid -.-> CHEBI_30753
ing24_Lipoic_acid["Lipoic acid"]
CultureMech_002227 --> ing24_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing24_Lipoic_acid -.-> CHEBI_16494
ing25_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002227 --> ing25_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing25_Nitrilotriacetic_acid -.-> CHEBI_44557
ing26_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002227 --> ing26_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing26_MnSO4_x_n_H2O -.-> CHEBI_86360
ing27_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002227 --> ing27_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing27_FeSO4_x_7_H2O -.-> CHEBI_75836
ing28_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002227 --> ing28_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing28_CoSO4_x_7_H2O -.-> CHEBI_91244
ing29_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002227 --> ing29_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing29_ZnSO4_x_7_H2O -.-> CHEBI_32312
CultureMech_002227 --> more["...4 more"]
Ingredients (34)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 1.99005 G_PER_L | ||
| 2 | Tryptone | 1.99005 G_PER_L | ||
| 3 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
4.97512 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 4 | PIPES | 3.44279 G_PER_L |
CHEBI:39033
PIPES
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
22.8905 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 21.8905, 1.0] |
| 6 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.98507 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
8.97015 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 5.97015, 3.0] |
| 8 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.995025 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.199005 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 10 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.39850700000000006 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.298507, 0.1] |
| 11 |
KCl
Cl.K 74.551 g/mol
|
0.497512 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 12 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.41791 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 13 |
NaBr
Br.Na 102.894 g/mol
|
0.0497512 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 14 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.0199005 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 15 | Ammonium ferric citrate | 0.00995025 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
|
| 16 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 17 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 18 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 19 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 20 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 21 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 22 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 23 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 24 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 25 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 26 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 27 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 28 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 29 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 30 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 31 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 32 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 33 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 34 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Adjust pH to 7.0. Distribute the medium into culture vessels under a N2 gas atmosphere, seal with butyl rubber stoppers and autoclave.
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.421345Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1042 |
| 2026-03-13T03:09:17.217471Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:11.439235Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002227 |
| 2026-03-17T06:30:25.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:24.231132+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CALROANAEROBACTER MEDIUM" to "calroanaerobacter_medium" for consistent matching |
| 2026-06-05T07:40:52.411570+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.266937+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |