Overview
- External ontology
- mediadive.medium:J1088 (source link unavailable) — 0.3 x HYPHOMICROBIUM MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1088
Composition
flowchart LR
CultureMech_002268["0_3_x_hyphomicrobium_medium"]
ing0_K2HPO4["K2HPO4"]
CultureMech_002268 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_NaH2PO4["NaH2PO4"]
CultureMech_002268 --> ing1_NaH2PO4
CHEBI_37585["CHEBI:37585"]
ing1_NaH2PO4 -.-> CHEBI_37585
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_002268 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002268 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_Methanol["Methanol"]
CultureMech_002268 --> ing4_Methanol
CHEBI_17790["CHEBI:17790"]
ing4_Methanol -.-> CHEBI_17790
ing5_Agar["Agar"]
CultureMech_002268 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_Vitamin_B12["Vitamin B12"]
CultureMech_002268 --> ing6_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing6_Vitamin_B12 -.-> CHEBI_176843
ing7_Thiamine_HCl["Thiamine HCl"]
CultureMech_002268 --> ing7_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing7_Thiamine_HCl -.-> CHEBI_49105
ing8_Folic_acid["Folic acid"]
CultureMech_002268 --> ing8_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing8_Folic_acid -.-> CHEBI_27470
ing9_Ascorbic_acid["Ascorbic acid"]
CultureMech_002268 --> ing9_Ascorbic_acid
CHEBI_22652["CHEBI:22652"]
ing9_Ascorbic_acid -.-> CHEBI_22652
ing10_Riboflavin["Riboflavin"]
CultureMech_002268 --> ing10_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing10_Riboflavin -.-> CHEBI_17015
ing11_Niacin["Niacin"]
CultureMech_002268 --> ing11_Niacin
CHEBI_15940["CHEBI:15940"]
ing11_Niacin -.-> CHEBI_15940
ing12_Pantothenic_acid["Pantothenic acid"]
CultureMech_002268 --> ing12_Pantothenic_acid
CHEBI_7916["CHEBI:7916"]
ing12_Pantothenic_acid -.-> CHEBI_7916
ing13_Na2_EDTA["Na2-EDTA"]
CultureMech_002268 --> ing13_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing13_Na2_EDTA -.-> CHEBI_64734
ing14_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002268 --> ing14_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing14_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing15_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002268 --> ing15_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing15_CaCl2_x_2_H2O -.-> CHEBI_86158
ing16_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002268 --> ing16_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing16_MnCl2_x_4_H2O -.-> CHEBI_86368
ing17_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002268 --> ing17_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing17_FeSO4_x_7_H2O -.-> CHEBI_75836
ing18__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
CultureMech_002268 --> ing18__NH4_6Mo7O24_x_4_H2O
CHEBI_91249["CHEBI:91249"]
ing18__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
ing19_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002268 --> ing19_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing19_CuSO4_x_5_H2O -.-> CHEBI_31440
ing20_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002268 --> ing20_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing20_CoCl2_x_6_H2O -.-> CHEBI_53503
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.845771 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 |
NaH2PO4
H2O4P.Na 119.976 g/mol
|
0.746269 G_PER_L |
CHEBI:37585
sodium dihydrogenphosphate
|
|
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.199005 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.0696517 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
Methanol
CH4O 32.042 g/mol
|
2 G_PER_L |
CHEBI:17790
methanol
|
|
| 6 | Agar | 14.9254 G_PER_L |
CHEBI:2509
agar
|
|
| 7 | Vitamin B12 | 0.25 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 8 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 9 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.025 G_PER_L |
CHEBI:27470
folic acid
|
|
| 10 | Ascorbic acid | 0.08 G_PER_L |
CHEBI:22652
ascorbic acid
|
|
| 11 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 12 |
Niacin
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 13 |
Pantothenic acid
C9H17NO5 219.237 g/mol
|
0.05 G_PER_L |
CHEBI:7916
pantothenic acid
|
|
| 14 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
10 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 15 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
4.4 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 16 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.466 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 17 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
1.012 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 18 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.998 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 19 |
(NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
|
0.22 G_PER_L |
CHEBI:91249
ammonium molybdate
|
|
| 20 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.314 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 21 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.322 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Preparation
- After autoclaving, aseptically add filter-sterilized trace vitamins solution and methanol.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- 0_3_x_hyphomicrobium_medium (CultureMech:002268)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.587341Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1088 |
| 2026-03-13T06:19:11.806990Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002268 |
| 2026-03-17T06:30:53.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:24.676588+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "0.3 x HYPHOMICROBIUM MEDIUM" to "0_3_x_hyphomicrobium_medium" for consistent matching |
| 2026-06-05T07:40:15.250972+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.434959+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:33.111398+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 45a350b4fc9bc7ee93efaedcb7b38594d50064a8ec5841532ed59e9c8255c6d3 |