0_3_x_hyphomicrobium_medium

CultureMech:002268 bacterial DEFINED SOLID_AGAR
Original name: 0.3 x HYPHOMICROBIUM MEDIUM

Overview

External ontology
mediadive.medium:J1088 (source link unavailable) — 0.3 x HYPHOMICROBIUM MEDIUM
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1088

Composition

flowchart LR
    CultureMech_002268["0_3_x_hyphomicrobium_medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_002268 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_NaH2PO4["NaH2PO4"]
    CultureMech_002268 --> ing1_NaH2PO4
    CHEBI_37585["CHEBI:37585"]
    ing1_NaH2PO4 -.-> CHEBI_37585
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002268 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002268 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_Methanol["Methanol"]
    CultureMech_002268 --> ing4_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing4_Methanol -.-> CHEBI_17790
    ing5_Agar["Agar"]
    CultureMech_002268 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_Vitamin_B12["Vitamin B12"]
    CultureMech_002268 --> ing6_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing6_Vitamin_B12 -.-> CHEBI_176843
    ing7_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002268 --> ing7_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing7_Thiamine_HCl -.-> CHEBI_49105
    ing8_Folic_acid["Folic acid"]
    CultureMech_002268 --> ing8_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing8_Folic_acid -.-> CHEBI_27470
    ing9_Ascorbic_acid["Ascorbic acid"]
    CultureMech_002268 --> ing9_Ascorbic_acid
    CHEBI_22652["CHEBI:22652"]
    ing9_Ascorbic_acid -.-> CHEBI_22652
    ing10_Riboflavin["Riboflavin"]
    CultureMech_002268 --> ing10_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing10_Riboflavin -.-> CHEBI_17015
    ing11_Niacin["Niacin"]
    CultureMech_002268 --> ing11_Niacin
    CHEBI_15940["CHEBI:15940"]
    ing11_Niacin -.-> CHEBI_15940
    ing12_Pantothenic_acid["Pantothenic acid"]
    CultureMech_002268 --> ing12_Pantothenic_acid
    CHEBI_7916["CHEBI:7916"]
    ing12_Pantothenic_acid -.-> CHEBI_7916
    ing13_Na2_EDTA["Na2-EDTA"]
    CultureMech_002268 --> ing13_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing13_Na2_EDTA -.-> CHEBI_64734
    ing14_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002268 --> ing14_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing14_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing15_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002268 --> ing15_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing15_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing16_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002268 --> ing16_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing16_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing17_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002268 --> ing17_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing17_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing18__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_002268 --> ing18__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing18__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing19_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002268 --> ing19_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing19_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing20_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002268 --> ing20_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing20_CoCl2_x_6_H2O -.-> CHEBI_53503

Ingredients (21)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
0.845771 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
2 NaH2PO4
H2O4P.Na 119.976 g/mol
0.746269 G_PER_L CHEBI:37585
sodium dihydrogenphosphate
3 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.199005 G_PER_L CHEBI:62946
ammonium sulfate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.0696517 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 Methanol
CH4O 32.042 g/mol
2 G_PER_L CHEBI:17790
methanol
6 Agar 14.9254 G_PER_L CHEBI:2509
agar
7 Vitamin B12 0.25 G_PER_L CHEBI:176843
vitamin B12
8 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.05 G_PER_L CHEBI:49105
thiamine hydrochloride
9 Folic acid
C19H19N7O6 441.404 g/mol
0.025 G_PER_L CHEBI:27470
folic acid
10 Ascorbic acid 0.08 G_PER_L CHEBI:22652
ascorbic acid
11 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
12 Niacin
C6H5NO2 123.111 g/mol
0.05 G_PER_L CHEBI:15940
nicotinic acid
13 Pantothenic acid
C9H17NO5 219.237 g/mol
0.05 G_PER_L CHEBI:7916
pantothenic acid
14 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
10 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
15 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
4.4 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
16 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1.466 G_PER_L CHEBI:86158
calcium chloride dihydrate
17 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
1.012 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
18 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.998 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
19 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
0.22 G_PER_L CHEBI:91249
ammonium molybdate
20 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.314 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
21 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.322 G_PER_L CHEBI:53503
cobalt chloride hexahydrate

Preparation

  1. After autoclaving, aseptically add filter-sterilized trace vitamins solution and methanol.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.587341Z mediadive-import Imported from MediaDive Source: JCM, ID: J1088
2026-03-13T06:19:11.806990Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002268
2026-03-17T06:30:53.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:24.676588+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "0.3 x HYPHOMICROBIUM MEDIUM" to "0_3_x_hyphomicrobium_medium" for consistent matching
2026-06-05T07:40:15.250972+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:42.434959+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.