pyga_medium

CultureMech:002275 bacterial COMPLEX SOLID_AGAR
Original name: PYGA MEDIUM

Overview

External ontology
mediadive.medium:J1096 (source link unavailable) — PYGA MEDIUM
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1096

Composition

flowchart LR
    CultureMech_002275["pyga_medium"]
    ing0_Peptone["Peptone"]
    CultureMech_002275 --> ing0_Peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002275 --> ing1_Yeast_extract
    ing2_K2HPO4["K2HPO4"]
    CultureMech_002275 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_Glucose["Glucose"]
    CultureMech_002275 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Agar["Agar"]
    CultureMech_002275 --> ing4_Agar
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar -.-> CHEBI_2509
    ing5_NaHCO3["NaHCO3"]
    CultureMech_002275 --> ing5_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing5_NaHCO3 -.-> CHEBI_32139
    ing6_Na2CO3["Na2CO3"]
    CultureMech_002275 --> ing6_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing6_Na2CO3 -.-> CHEBI_29377

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Peptone 8.88889 G_PER_L
2 Yeast extract 3.33333 G_PER_L
3 K2HPO4
HO4P.2K 174.174 g/mol
1.11111 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 Glucose
C6H12O6 180.156 g/mol
11.1111 G_PER_L CHEBI:17234
glucose
5 Agar 16.6667 G_PER_L CHEBI:2509
agar
6 NaHCO3
CHO3.Na 84.006 g/mol
29 G_PER_L CHEBI:32139
sodium hydrogencarbonate
7 Na2CO3
CO3.2Na 105.988 g/mol
70 G_PER_L CHEBI:29377
sodium carbonate

Preparation

  1. Autoclave Solutions 1 and 2 separately and mix them aseptically.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:18.613785Z mediadive-import Imported from MediaDive Source: JCM, ID: J1096
2026-03-13T06:19:11.870481Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002275
2026-03-17T06:30:25.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:24.750044+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PYGA MEDIUM" to "pyga_medium" for consistent matching
2026-06-05T07:41:43.129775+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.613530+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:53.976272+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint b46b9c8012cee05c5aab6d3b1fb9a7e060154b3022614c45f368e718487fd700