Overview
- External ontology
- mediadive.medium:J1096 (source link unavailable) — PYGA MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1096
Composition
flowchart LR
CultureMech_002275["pyga_medium"]
ing0_Peptone["Peptone"]
CultureMech_002275 --> ing0_Peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_002275 --> ing1_Yeast_extract
ing2_K2HPO4["K2HPO4"]
CultureMech_002275 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_Glucose["Glucose"]
CultureMech_002275 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Agar["Agar"]
CultureMech_002275 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
ing5_NaHCO3["NaHCO3"]
CultureMech_002275 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_Na2CO3["Na2CO3"]
CultureMech_002275 --> ing6_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing6_Na2CO3 -.-> CHEBI_29377
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 8.88889 G_PER_L | ||
| 2 | Yeast extract | 3.33333 G_PER_L | ||
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1.11111 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
11.1111 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 | Agar | 16.6667 G_PER_L |
CHEBI:2509
agar
|
|
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
29 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 7 |
Na2CO3
CO3.2Na 105.988 g/mol
|
70 G_PER_L |
CHEBI:29377
sodium carbonate
|
Preparation
- Autoclave Solutions 1 and 2 separately and mix them aseptically.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.613785Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1096 |
| 2026-03-13T06:19:11.870481Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002275 |
| 2026-03-17T06:30:25.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:24.750044+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYGA MEDIUM" to "pyga_medium" for consistent matching |
| 2026-06-05T07:41:43.129775+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.613530+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |