ctm_medium

CultureMech:002279 bacterial COMPLEX LIQUID
Original name: CTM MEDIUM

Overview

External ontology
mediadive.medium:J1100 (source link unavailable) — CTM MEDIUM
Applications
  • Microbial cultivation
pH
7.0
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1100

Composition

flowchart LR
    CultureMech_002279["ctm_medium"]
    ing0_HEPES["HEPES"]
    CultureMech_002279 --> ing0_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing0_HEPES -.-> CHEBI_46756
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002279 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002279 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_Citric_acid["Citric acid"]
    CultureMech_002279 --> ing3_Citric_acid
    CHEBI_30769["CHEBI:30769"]
    ing3_Citric_acid -.-> CHEBI_30769
    ing4_Na2_EDTA["Na2-EDTA"]
    CultureMech_002279 --> ing4_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing4_Na2_EDTA -.-> CHEBI_64734
    ing5_H3BO3["H3BO3"]
    CultureMech_002279 --> ing5_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing5_H3BO3 -.-> CHEBI_33118
    ing6_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002279 --> ing6_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing6_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002279 --> ing7_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing8_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002279 --> ing8_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing8_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing9_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002279 --> ing9_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing9_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing10_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_002279 --> ing10_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing10_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing11_K2HPO4["K2HPO4"]
    CultureMech_002279 --> ing11_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing11_K2HPO4 -.-> CHEBI_131527
    ing12_Ferric_ammonium_citrate["Ferric ammonium citrate"]
    CultureMech_002279 --> ing12_Ferric_ammonium_citrate
    CHEBI_31604["CHEBI:31604"]
    ing12_Ferric_ammonium_citrate -.-> CHEBI_31604
    ing13_2_Oxoglutarate["2-Oxoglutarate"]
    CultureMech_002279 --> ing13_2_Oxoglutarate
    CHEBI_16810["CHEBI:16810"]
    ing13_2_Oxoglutarate -.-> CHEBI_16810
    ing14_NaHCO3["NaHCO3"]
    CultureMech_002279 --> ing14_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing14_NaHCO3 -.-> CHEBI_32139
    ing15_Sodium_thioglycolate["Sodium thioglycolate"]
    CultureMech_002279 --> ing15_Sodium_thioglycolate
    CHEBI_86481["CHEBI:86481"]
    ing15_Sodium_thioglycolate -.-> CHEBI_86481
    ing16_Bacto_peptone["Bacto peptone"]
    CultureMech_002279 --> ing16_Bacto_peptone
    ing17_L_Alanine["L-Alanine"]
    CultureMech_002279 --> ing17_L_Alanine
    CHEBI_16977["CHEBI:16977"]
    ing17_L_Alanine -.-> CHEBI_16977
    ing18_L_Arginine["L-Arginine"]
    CultureMech_002279 --> ing18_L_Arginine
    CHEBI_16467["CHEBI:16467"]
    ing18_L_Arginine -.-> CHEBI_16467
    ing19_L_Asparagine["L-Asparagine"]
    CultureMech_002279 --> ing19_L_Asparagine
    CHEBI_17196["CHEBI:17196"]
    ing19_L_Asparagine -.-> CHEBI_17196
    ing20_L_Aspartic_acid["L-Aspartic acid"]
    CultureMech_002279 --> ing20_L_Aspartic_acid
    CHEBI_17053["CHEBI:17053"]
    ing20_L_Aspartic_acid -.-> CHEBI_17053
    ing21_L_Cysteine["L-Cysteine"]
    CultureMech_002279 --> ing21_L_Cysteine
    CHEBI_17561["CHEBI:17561"]
    ing21_L_Cysteine -.-> CHEBI_17561
    ing22_L_Glutamic_acid["L-Glutamic acid"]
    CultureMech_002279 --> ing22_L_Glutamic_acid
    CHEBI_16015["CHEBI:16015"]
    ing22_L_Glutamic_acid -.-> CHEBI_16015
    ing23_L_Glutamine["L-Glutamine"]
    CultureMech_002279 --> ing23_L_Glutamine
    CHEBI_18050["CHEBI:18050"]
    ing23_L_Glutamine -.-> CHEBI_18050
    ing24_Glycine["Glycine"]
    CultureMech_002279 --> ing24_Glycine
    CHEBI_15428["CHEBI:15428"]
    ing24_Glycine -.-> CHEBI_15428
    ing25_L_Histidine["L-Histidine"]
    CultureMech_002279 --> ing25_L_Histidine
    CHEBI_15971["CHEBI:15971"]
    ing25_L_Histidine -.-> CHEBI_15971
    ing26_L_Isoleucine["L-Isoleucine"]
    CultureMech_002279 --> ing26_L_Isoleucine
    CHEBI_17191["CHEBI:17191"]
    ing26_L_Isoleucine -.-> CHEBI_17191
    ing27_L_Leucine["L-Leucine"]
    CultureMech_002279 --> ing27_L_Leucine
    CHEBI_15603["CHEBI:15603"]
    ing27_L_Leucine -.-> CHEBI_15603
    ing28_L_Lysine["L-Lysine"]
    CultureMech_002279 --> ing28_L_Lysine
    CHEBI_18019["CHEBI:18019"]
    ing28_L_Lysine -.-> CHEBI_18019
    ing29_L_Methionine["L-Methionine"]
    CultureMech_002279 --> ing29_L_Methionine
    CHEBI_16643["CHEBI:16643"]
    ing29_L_Methionine -.-> CHEBI_16643
    CultureMech_002279 --> more["...22 more"]

Ingredients (52)

# Ingredient Concentration Ontology Notes
1 HEPES 85.7143 G_PER_L CHEBI:46756
HEPES
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3.56803 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1.71265 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 Citric acid
C6H8O7 192.123 g/mol
0.285442 G_PER_L CHEBI:30769
citric acid
5 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.6 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
6 H3BO3
H3BO3 61.833 g/mol
2.86 G_PER_L CHEBI:33118
boric acid
7 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
1.81 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
8 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.222 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
9 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.39 G_PER_L CHEBI:75213
sodium molybdate dihydrate
10 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.079 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
11 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
0.049 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate
12 K2HPO4
HO4P.2K 174.174 g/mol
15.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
13 Ferric ammonium citrate 12 G_PER_L CHEBI:31604
ferric ammonium citrate
14 2-Oxoglutarate
C5H4O5 144.082 g/mol
14.6 G_PER_L CHEBI:16810
2-oxoglutarate(2-)
15 NaHCO3
CHO3.Na 84.006 g/mol
56.8182 G_PER_L CHEBI:32139
sodium hydrogencarbonate
16 Sodium thioglycolate
C2H3O2S.Na 114.101 g/mol
11.3636 G_PER_L CHEBI:86481
sodium thioglycolate
17 Bacto peptone 100 G_PER_L
18 L-Alanine
C3H7NO2 89.094 g/mol
0.25 G_PER_L CHEBI:16977
L-alanine
19 L-Arginine
C6H14N4O2 174.204 g/mol
0.25 G_PER_L CHEBI:16467
L-arginine
20 L-Asparagine
C4H8N2O3 132.119 g/mol
0.25 G_PER_L CHEBI:17196
L-asparagine
21 L-Aspartic acid
C4H7NO4 133.103 g/mol
0.25 G_PER_L CHEBI:17053
L-aspartic acid
22 L-Cysteine
C3H7NO2S 121.161 g/mol
0.25 G_PER_L CHEBI:17561
L-cysteine
23 L-Glutamic acid
C5H9NO4 147.13 g/mol
0.25 G_PER_L CHEBI:16015
L-glutamic acid
24 L-Glutamine
C5H10N2O3 146.146 g/mol
0.25 G_PER_L CHEBI:18050
L-glutamine
25 Glycine
C2H5NO2 75.067 g/mol
0.25 G_PER_L CHEBI:15428
glycine
26 L-Histidine
C6H9N3O2 155.157 g/mol
0.25 G_PER_L CHEBI:15971
L-histidine
27 L-Isoleucine
C6H13NO2 131.175 g/mol
0.25 G_PER_L CHEBI:17191
L-isoleucine
28 L-Leucine
C6H13NO2 131.175 g/mol
0.25 G_PER_L CHEBI:15603
L-leucine
29 L-Lysine
C6H14N2O2 146.19 g/mol
0.25 G_PER_L CHEBI:18019
L-lysine
30 L-Methionine
C5H11NO2S 149.215 g/mol
0.25 G_PER_L CHEBI:16643
L-methionine
31 L-Phenylalanine
C9H11NO2 165.192 g/mol
0.25 G_PER_L CHEBI:17295
L-phenylalanine
32 L-Proline
C5H9NO2 115.132 g/mol
0.25 G_PER_L CHEBI:17203
L-proline
33 L-Serine
C3H7NO3 105.093 g/mol
0.25 G_PER_L CHEBI:17115
L-serine
34 L-Threonine
C4H9NO3 119.12 g/mol
0.25 G_PER_L CHEBI:16857
L-threonine
35 L-Tryptophan
C11H12N2O2 204.229 g/mol
0.25 G_PER_L CHEBI:16828
L-tryptophan
36 L-Tyrosine
C9H11NO3 181.191 g/mol
0.25 G_PER_L CHEBI:17895
L-tyrosine
37 L-Valine
C5H11NO2 117.148 g/mol
0.25 G_PER_L CHEBI:16414
L-valine
38 Biotin
C10H16N2O3S 244.316 g/mol
0.1 G_PER_L CHEBI:15956
biotin
39 Riboflavin
C17H20N4O6 376.369 g/mol
0.1 G_PER_L CHEBI:17015
riboflavin
40 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
1 G_PER_L CHEBI:49105
thiamine hydrochloride
41 Thiamine pyrophosphate
C12H19N4O7P2S.Cl 460.773 g/mol
1 G_PER_L CHEBI:18290
thiamine(1+) diphosphate chloride
42 L-Ascorbic acid
C6H8O6 176.124 g/mol
1 G_PER_L CHEBI:29073
L-ascorbic acid
43 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
1 G_PER_L CHEBI:31345
Calcium pantothenate
44 Folic acid
C19H19N7O6 441.404 g/mol
1 G_PER_L CHEBI:27470
folic acid
45 Nicotinamide
C6H6N2O 122.127 g/mol
1 G_PER_L CHEBI:17154
nicotinamide
46 Nicotinic acid
C6H5NO2 123.111 g/mol
1 G_PER_L CHEBI:15940
nicotinic acid
47 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
1 G_PER_L CHEBI:30753
4-aminobenzoic acid
48 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
1 G_PER_L CHEBI:30961
pyridoxine hydrochloride
49 Lipoic acid
C8H14O2S2 206.332 g/mol
1 G_PER_L CHEBI:16494
lipoic acid
50 beta-NAD
C21H28N7O14P2 664.438 g/mol
1 G_PER_L CHEBI:15846
NAD(+)
51 Potassium phosphate buffer 100 G_PER_L
52 Vitamin B12 1 G_PER_L CHEBI:176843
vitamin B12

Preparation

  1. CTM medium basis:
  2. Mix all ingredients in 1.0 L medium bottle. Add distilled water close to 1.0 L. Adjust pH to 7.0 with 2 M KOH and fill up to 1.0 L. Autoclave at 121C for 40 min. After autoclaving, seal medium bottle immediately in order to avoid oxygenation. Cool down to around 70C. Add 30 ml filter-sterilized Mixed solution (see below) to the autoclaved CTM medium basis for completing the CTM medium.
  3. Add distilled water to bring final volume to 30 ml.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:18.627406Z mediadive-import Imported from MediaDive Source: JCM, ID: J1100
2026-03-13T06:19:11.916263Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002279
2026-03-17T06:30:29.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:24.803280+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CTM MEDIUM" to "ctm_medium" for consistent matching
2026-06-05T07:40:54.990157+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 50 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:55.026680+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:29.316317+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 057c0b18e6f597c3e7235d7ef889ad1aad2a5cc1bbfe760b302ed1b7c88bdc5b