ctm_medium

CultureMech:002279 bacterial COMPLEX LIQUID
Original name: CTM MEDIUM

Overview

External ontology
mediadive.medium:J1100 (source link unavailable) — CTM MEDIUM
Applications
  • Microbial cultivation
pH
7.0
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1100

Composition

flowchart LR
    CultureMech_002279["ctm_medium"]
    ing0_HEPES["HEPES"]
    CultureMech_002279 --> ing0_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing0_HEPES -.-> CHEBI_46756
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002279 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002279 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_Citric_acid["Citric acid"]
    CultureMech_002279 --> ing3_Citric_acid
    CHEBI_30769["CHEBI:30769"]
    ing3_Citric_acid -.-> CHEBI_30769
    ing4_Na2_EDTA["Na2-EDTA"]
    CultureMech_002279 --> ing4_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing4_Na2_EDTA -.-> CHEBI_64734
    ing5_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002279 --> ing5_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing5_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing6_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002279 --> ing6_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing6_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing7_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002279 --> ing7_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing7_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing8_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_002279 --> ing8_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing8_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing9_K2HPO4["K2HPO4"]
    CultureMech_002279 --> ing9_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing9_K2HPO4 -.-> CHEBI_131527
    ing10_Ferric_ammonium_citrate["Ferric ammonium citrate"]
    CultureMech_002279 --> ing10_Ferric_ammonium_citrate
    CHEBI_31604["CHEBI:31604"]
    ing10_Ferric_ammonium_citrate -.-> CHEBI_31604
    ing11_2_Oxoglutarate["2-Oxoglutarate"]
    CultureMech_002279 --> ing11_2_Oxoglutarate
    CHEBI_16810["CHEBI:16810"]
    ing11_2_Oxoglutarate -.-> CHEBI_16810
    ing12_NaHCO3["NaHCO3"]
    CultureMech_002279 --> ing12_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing12_NaHCO3 -.-> CHEBI_32139
    ing13_Sodium_thioglycolate["Sodium thioglycolate"]
    CultureMech_002279 --> ing13_Sodium_thioglycolate
    CHEBI_86481["CHEBI:86481"]
    ing13_Sodium_thioglycolate -.-> CHEBI_86481
    ing14_Bacto_peptone["Bacto peptone"]
    CultureMech_002279 --> ing14_Bacto_peptone
    ing15_L_Alanine["L-Alanine"]
    CultureMech_002279 --> ing15_L_Alanine
    CHEBI_16977["CHEBI:16977"]
    ing15_L_Alanine -.-> CHEBI_16977
    ing16_L_Arginine["L-Arginine"]
    CultureMech_002279 --> ing16_L_Arginine
    CHEBI_16467["CHEBI:16467"]
    ing16_L_Arginine -.-> CHEBI_16467
    ing17_L_Asparagine["L-Asparagine"]
    CultureMech_002279 --> ing17_L_Asparagine
    CHEBI_17196["CHEBI:17196"]
    ing17_L_Asparagine -.-> CHEBI_17196
    ing18_L_Aspartic_acid["L-Aspartic acid"]
    CultureMech_002279 --> ing18_L_Aspartic_acid
    CHEBI_17053["CHEBI:17053"]
    ing18_L_Aspartic_acid -.-> CHEBI_17053
    ing19_L_Cysteine["L-Cysteine"]
    CultureMech_002279 --> ing19_L_Cysteine
    CHEBI_17561["CHEBI:17561"]
    ing19_L_Cysteine -.-> CHEBI_17561
    ing20_L_Glutamic_acid["L-Glutamic acid"]
    CultureMech_002279 --> ing20_L_Glutamic_acid
    CHEBI_16015["CHEBI:16015"]
    ing20_L_Glutamic_acid -.-> CHEBI_16015
    ing21_L_Glutamine["L-Glutamine"]
    CultureMech_002279 --> ing21_L_Glutamine
    CHEBI_18050["CHEBI:18050"]
    ing21_L_Glutamine -.-> CHEBI_18050
    ing22_Glycine["Glycine"]
    CultureMech_002279 --> ing22_Glycine
    CHEBI_15428["CHEBI:15428"]
    ing22_Glycine -.-> CHEBI_15428
    ing23_L_Histidine["L-Histidine"]
    CultureMech_002279 --> ing23_L_Histidine
    CHEBI_15971["CHEBI:15971"]
    ing23_L_Histidine -.-> CHEBI_15971
    ing24_L_Isoleucine["L-Isoleucine"]
    CultureMech_002279 --> ing24_L_Isoleucine
    CHEBI_17191["CHEBI:17191"]
    ing24_L_Isoleucine -.-> CHEBI_17191
    ing25_L_Leucine["L-Leucine"]
    CultureMech_002279 --> ing25_L_Leucine
    CHEBI_15603["CHEBI:15603"]
    ing25_L_Leucine -.-> CHEBI_15603
    ing26_L_Lysine["L-Lysine"]
    CultureMech_002279 --> ing26_L_Lysine
    CHEBI_18019["CHEBI:18019"]
    ing26_L_Lysine -.-> CHEBI_18019
    ing27_L_Methionine["L-Methionine"]
    CultureMech_002279 --> ing27_L_Methionine
    CHEBI_16643["CHEBI:16643"]
    ing27_L_Methionine -.-> CHEBI_16643
    ing28_L_Phenylalanine["L-Phenylalanine"]
    CultureMech_002279 --> ing28_L_Phenylalanine
    CHEBI_17295["CHEBI:17295"]
    ing28_L_Phenylalanine -.-> CHEBI_17295
    ing29_L_Proline["L-Proline"]
    CultureMech_002279 --> ing29_L_Proline
    CHEBI_17203["CHEBI:17203"]
    ing29_L_Proline -.-> CHEBI_17203
    CultureMech_002279 --> more["...10 more"]

Ingredients (40)

# Ingredient Concentration Ontology Notes
1 HEPES 85.7143 G_PER_L CHEBI:46756
HEPES
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3.56803 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1.71265 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 Citric acid
C6H8O7 192.123 g/mol
0.285442 G_PER_L CHEBI:30769
citric acid
5 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.6 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
6 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.222 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
7 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.39 G_PER_L CHEBI:75213
sodium molybdate dihydrate
8 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.079 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
9 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
0.049 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate
10 K2HPO4
HO4P.2K 174.174 g/mol
15.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
11 Ferric ammonium citrate 12 G_PER_L CHEBI:31604
ferric ammonium citrate
12 2-Oxoglutarate
C5H4O5 144.082 g/mol
14.6 G_PER_L CHEBI:16810
2-oxoglutarate(2-)
13 NaHCO3
CHO3.Na 84.006 g/mol
56.8182 G_PER_L CHEBI:32139
sodium hydrogencarbonate
14 Sodium thioglycolate
C2H3O2S.Na 114.101 g/mol
11.3636 G_PER_L CHEBI:86481
sodium thioglycolate
15 Bacto peptone 100 G_PER_L
16 L-Alanine
C3H7NO2 89.094 g/mol
0.25 G_PER_L CHEBI:16977
L-alanine
17 L-Arginine
C6H14N4O2 174.204 g/mol
0.25 G_PER_L CHEBI:16467
L-arginine
18 L-Asparagine
C4H8N2O3 132.119 g/mol
0.25 G_PER_L CHEBI:17196
L-asparagine
19 L-Aspartic acid
C4H7NO4 133.103 g/mol
0.25 G_PER_L CHEBI:17053
L-aspartic acid
20 L-Cysteine
C3H7NO2S 121.161 g/mol
0.25 G_PER_L CHEBI:17561
L-cysteine
21 L-Glutamic acid
C5H9NO4 147.13 g/mol
0.25 G_PER_L CHEBI:16015
L-glutamic acid
22 L-Glutamine
C5H10N2O3 146.146 g/mol
0.25 G_PER_L CHEBI:18050
L-glutamine
23 Glycine
C2H5NO2 75.067 g/mol
0.25 G_PER_L CHEBI:15428
glycine
24 L-Histidine
C6H9N3O2 155.157 g/mol
0.25 G_PER_L CHEBI:15971
L-histidine
25 L-Isoleucine
C6H13NO2 131.175 g/mol
0.25 G_PER_L CHEBI:17191
L-isoleucine
26 L-Leucine
C6H13NO2 131.175 g/mol
0.25 G_PER_L CHEBI:15603
L-leucine
27 L-Lysine
C6H14N2O2 146.19 g/mol
0.25 G_PER_L CHEBI:18019
L-lysine
28 L-Methionine
C5H11NO2S 149.215 g/mol
0.25 G_PER_L CHEBI:16643
L-methionine
29 L-Phenylalanine
C9H11NO2 165.192 g/mol
0.25 G_PER_L CHEBI:17295
L-phenylalanine
30 L-Proline
C5H9NO2 115.132 g/mol
0.25 G_PER_L CHEBI:17203
L-proline
31 L-Serine
C3H7NO3 105.093 g/mol
0.25 G_PER_L CHEBI:17115
L-serine
32 L-Threonine
C4H9NO3 119.12 g/mol
0.25 G_PER_L CHEBI:16857
L-threonine
33 L-Tryptophan
C11H12N2O2 204.229 g/mol
0.25 G_PER_L CHEBI:16828
L-tryptophan
34 L-Tyrosine
C9H11NO3 181.191 g/mol
0.25 G_PER_L CHEBI:17895
L-tyrosine
35 L-Valine
C5H11NO2 117.148 g/mol
0.25 G_PER_L CHEBI:16414
L-valine
36 L-Ascorbic acid
C6H8O6 176.124 g/mol
1 G_PER_L CHEBI:29073
L-ascorbic acid
37 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
1 G_PER_L CHEBI:31345
Calcium pantothenate
38 Nicotinamide
C6H6N2O 122.127 g/mol
1 G_PER_L CHEBI:17154
nicotinamide
39 beta-NAD
C21H28N7O14P2 664.438 g/mol
1 G_PER_L CHEBI:15846
NAD(+)
40 Potassium phosphate buffer 100 G_PER_L

Preparation

  1. CTM medium basis:
  2. Mix all ingredients in 1.0 L medium bottle. Add distilled water close to 1.0 L. Adjust pH to 7.0 with 2 M KOH and fill up to 1.0 L. Autoclave at 121C for 40 min. After autoclaving, seal medium bottle immediately in order to avoid oxygenation. Cool down to around 70C. Add 30 ml filter-sterilized Mixed solution (see below) to the autoclaved CTM medium basis for completing the CTM medium.
  3. Add distilled water to bring final volume to 30 ml.

Solutions

Trace metal solution

Dose: 50 ML_PER_L

Stock composition
IngredientConcentrationNotes
H3BO3 2.86 G_PER_L
MnCl2 x 4 H2O 1.81 G_PER_L

Vitamin solution A (1000 x stock)

Dose: 0.25 ML_PER_L

Stock composition
IngredientConcentrationNotes
Biotin 0.1 G_PER_L
Riboflavin 0.1 G_PER_L
Thiamine HCl 1 G_PER_L
Thiamine pyrophosphate 1 G_PER_L
Folic acid 1 G_PER_L
Nicotinic acid 1 G_PER_L
p-Aminobenzoic acid 1 G_PER_L
Pyridoxine hydrochloride 1 G_PER_L
Lipoic acid 1 G_PER_L

Vitamin solution B (1000 x stock)

Dose: 0.25 ML_PER_L

Stock composition
IngredientConcentrationNotes
Vitamin B12 1 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.627406Z mediadive-import Imported from MediaDive Source: JCM, ID: J1100
2026-03-13T06:19:11.916263Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002279
2026-03-17T06:30:29.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:24.803280+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CTM MEDIUM" to "ctm_medium" for consistent matching
2026-06-05T07:40:54.990157+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 50 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:55.026680+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-13T23:31:54.359024+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 12 stock-strength component(s) out of ingredients into 3 solution(s): 2 -> 'Trace metal solution' @ 50 ml/l; 9 -> 'Vitamin solution A (1000 x stock)' @ 0.25 ml/l; 1 -> 'Vitamin solution B (1000 x stock)' @ 0.25 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).