Overview
- External ontology
- mediadive.medium:J1107 (source link unavailable) — LIQUID BASAL MEDIUM FOR METHANOSARCINA
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1107
Composition
flowchart LR
CultureMech_002284["liquid_basal_medium_for_methanosarcina"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002284 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_NH4Cl["NH4Cl"]
CultureMech_002284 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002284 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002284 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_NaCl["NaCl"]
CultureMech_002284 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_MOPS["MOPS"]
CultureMech_002284 --> ing5_MOPS
CHEBI_39074["CHEBI:39074"]
ing5_MOPS -.-> CHEBI_39074
ing6_Resazurin["Resazurin"]
CultureMech_002284 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_NaHCO3["NaHCO3"]
CultureMech_002284 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_Methanol["Methanol"]
CultureMech_002284 --> ing8_Methanol
CHEBI_17790["CHEBI:17790"]
ing8_Methanol -.-> CHEBI_17790
ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002284 --> ing9_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002284 --> ing10_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing10_Na2S_x_9_H2O -.-> CHEBI_76209
ing11_Nitrilotriacetic_acid_trisodium_salt_monohydrate["Nitrilotriacetic acid trisodium salt monohydrate"]
CultureMech_002284 --> ing11_Nitrilotriacetic_acid_trisodium_salt_monohydrate
ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002284 --> ing12_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
ing13_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002284 --> ing13_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing13_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002284 --> ing14_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
ing15_MnSO4_x_5_H2O["MnSO4 x 5 H2O"]
CultureMech_002284 --> ing15_MnSO4_x_5_H2O
CHEBI_131524["CHEBI:131524"]
ing15_MnSO4_x_5_H2O -.-> CHEBI_131524
ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002284 --> ing16_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing17_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002284 --> ing17_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing17_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing18_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002284 --> ing18_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing18_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002284 --> ing19_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
ing20_H3BO3["H3BO3"]
CultureMech_002284 --> ing20_H3BO3
CHEBI_33118["CHEBI:33118"]
ing20_H3BO3 -.-> CHEBI_33118
ing21_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002284 --> ing21_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing21_CuSO4_x_5_H2O -.-> CHEBI_31440
ing22_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002284 --> ing22_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing22_p_Aminobenzoic_acid -.-> CHEBI_30753
ing23_Nicotinic_acid["Nicotinic acid"]
CultureMech_002284 --> ing23_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing23_Nicotinic_acid -.-> CHEBI_15940
ing24_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
CultureMech_002284 --> ing24_DL_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing24_DL_Calcium_pantothenate -.-> CHEBI_31345
ing25_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002284 --> ing25_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing25_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing26_Riboflavin["Riboflavin"]
CultureMech_002284 --> ing26_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing26_Riboflavin -.-> CHEBI_17015
ing27_Thiamine_HCl["Thiamine HCl"]
CultureMech_002284 --> ing27_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing27_Thiamine_HCl -.-> CHEBI_49105
ing28_Biotin["Biotin"]
CultureMech_002284 --> ing28_Biotin
CHEBI_15956["CHEBI:15956"]
ing28_Biotin -.-> CHEBI_15956
ing29_Folic_acid["Folic acid"]
CultureMech_002284 --> ing29_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing29_Folic_acid -.-> CHEBI_27470
CultureMech_002284 --> more["...2 more"]
Ingredients (32)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.489716 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.391773 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.391773 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0489716 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
0.391773 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 | MOPS | 4.11361 G_PER_L |
CHEBI:39074
MOPS
|
|
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000979432 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
50 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 9 |
Methanol
CH4O 32.042 g/mol
|
10 G_PER_L |
CHEBI:17790
methanol
|
|
| 10 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
10 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 11 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
10 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 12 | Nitrilotriacetic acid trisodium salt monohydrate | 11.1 G_PER_L | ||
| 13 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
5.55 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 14 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.3 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 15 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
1 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 16 |
MnSO4 x 5 H2O
5H2O.Mn.O4S 241.076 g/mol
|
1 G_PER_L |
CHEBI:131524
manganese(II) sulfate pentahydrate
|
|
| 17 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 18 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
1 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 19 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 20 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
3 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 21 |
H3BO3
H3BO3 61.833 g/mol
|
0.1 G_PER_L |
CHEBI:33118
boric acid
|
|
| 22 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.1 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 23 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.01 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 24 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.01 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 25 |
DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.01 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 26 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 27 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.01 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 28 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.01 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 29 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.005 G_PER_L |
CHEBI:15956
biotin
|
|
| 30 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.005 G_PER_L |
CHEBI:27470
folic acid
|
|
| 31 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 32 | Vitamin B12 | 0.005 G_PER_L |
CHEBI:176843
vitamin B12
|
Preparation
- Mix components thoroughly, adjust pH to 7.2 and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
- Aseptically distribute the medium into culture vessels under the same gas mixture and seal with butyl rubber stoppers. Finally, add per liter the following solutions from anaerobic stocks (autoclaved and stored under a N2 atmosphere):
- Readjust pH to 7.2, if necessary.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- liquid_basal_medium_for_methanosarcina (CultureMech:002284)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.656550Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1107 |
| 2026-03-13T06:19:11.983090Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002284 |
| 2026-03-17T06:29:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:24.878982+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LIQUID BASAL MEDIUM FOR METHANOSARCINA" to "liquid_basal_medium_for_methanosarcina" for consistent matching |
| 2026-06-05T07:41:11.219149+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.564447+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:29.134173+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint f1ee1dcc27b9dd906032ed478d6094c099123a4731a66fad795b2df51128772f |