liquid_basal_medium_for_methanosarcina

CultureMech:002284 archaea DEFINED LIQUID
Original name: LIQUID BASAL MEDIUM FOR METHANOSARCINA

Overview

External ontology
mediadive.medium:J1107 (source link unavailable) — LIQUID BASAL MEDIUM FOR METHANOSARCINA
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1107

Composition

flowchart LR
    CultureMech_002284["liquid_basal_medium_for_methanosarcina"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002284 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NH4Cl["NH4Cl"]
    CultureMech_002284 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002284 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002284 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_NaCl["NaCl"]
    CultureMech_002284 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_MOPS["MOPS"]
    CultureMech_002284 --> ing5_MOPS
    CHEBI_39074["CHEBI:39074"]
    ing5_MOPS -.-> CHEBI_39074
    ing6_Resazurin["Resazurin"]
    CultureMech_002284 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_NaHCO3["NaHCO3"]
    CultureMech_002284 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139
    ing8_Methanol["Methanol"]
    CultureMech_002284 --> ing8_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing8_Methanol -.-> CHEBI_17790
    ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002284 --> ing9_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002284 --> ing10_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing10_Na2S_x_9_H2O -.-> CHEBI_76209
    ing11_Nitrilotriacetic_acid_trisodium_salt_monohydrate["Nitrilotriacetic acid trisodium salt monohydrate"]
    CultureMech_002284 --> ing11_Nitrilotriacetic_acid_trisodium_salt_monohydrate
    ing12_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002284 --> ing12_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing12_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing13_H3BO3["H3BO3"]
    CultureMech_002284 --> ing13_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing13_H3BO3 -.-> CHEBI_33118
    ing14_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002284 --> ing14_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing14_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing15_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002284 --> ing15_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing15_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing16_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002284 --> ing16_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing16_Nicotinic_acid -.-> CHEBI_15940
    ing17_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
    CultureMech_002284 --> ing17_DL_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing17_DL_Calcium_pantothenate -.-> CHEBI_31345
    ing18_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002284 --> ing18_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing18_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing19_Riboflavin["Riboflavin"]
    CultureMech_002284 --> ing19_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing19_Riboflavin -.-> CHEBI_17015
    ing20_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002284 --> ing20_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing20_Thiamine_HCl -.-> CHEBI_49105
    ing21_Biotin["Biotin"]
    CultureMech_002284 --> ing21_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing21_Biotin -.-> CHEBI_15956
    ing22_Folic_acid["Folic acid"]
    CultureMech_002284 --> ing22_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing22_Folic_acid -.-> CHEBI_27470
    ing23_Lipoic_acid["Lipoic acid"]
    CultureMech_002284 --> ing23_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing23_Lipoic_acid -.-> CHEBI_16494
    ing24_Vitamin_B12["Vitamin B12"]
    CultureMech_002284 --> ing24_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing24_Vitamin_B12 -.-> CHEBI_176843

Ingredients (25)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.489716 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 NH4Cl
Cl.H4N 53.492 g/mol
0.391773 G_PER_L CHEBI:31206
ammonium chloride
3 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.391773 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0489716 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 NaCl
Cl.Na 58.443 g/mol
0.391773 G_PER_L CHEBI:26710
sodium chloride
6 MOPS 4.11361 G_PER_L CHEBI:39074
MOPS
7 Resazurin
C12H7NO4 229.191 g/mol
0.000979432 G_PER_L CHEBI:8806
Resazurin
8 NaHCO3
CHO3.Na 84.006 g/mol
50 G_PER_L CHEBI:32139
sodium hydrogencarbonate
9 Methanol
CH4O 32.042 g/mol
10 G_PER_L CHEBI:17790
methanol
10 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
10 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
11 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
10 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
12 Nitrilotriacetic acid trisodium salt monohydrate 11.1 G_PER_L
13 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.3 G_PER_L CHEBI:131361
disodium selenite pentahydrate
14 H3BO3
H3BO3 61.833 g/mol
0.1 G_PER_L CHEBI:33118
boric acid
15 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.1 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
16 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.01 G_PER_L CHEBI:30753
4-aminobenzoic acid
17 Nicotinic acid
C6H5NO2 123.111 g/mol
0.01 G_PER_L CHEBI:15940
nicotinic acid
18 DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.01 G_PER_L CHEBI:31345
Calcium pantothenate
19 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
20 Riboflavin
C17H20N4O6 376.369 g/mol
0.01 G_PER_L CHEBI:17015
riboflavin
21 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.01 G_PER_L CHEBI:49105
thiamine hydrochloride
22 Biotin
C10H16N2O3S 244.316 g/mol
0.005 G_PER_L CHEBI:15956
biotin
23 Folic acid
C19H19N7O6 441.404 g/mol
0.005 G_PER_L CHEBI:27470
folic acid
24 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid
25 Vitamin B12 0.005 G_PER_L CHEBI:176843
vitamin B12

Preparation

  1. Mix components thoroughly, adjust pH to 7.2 and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
  2. Aseptically distribute the medium into culture vessels under the same gas mixture and seal with butyl rubber stoppers. Finally, add per liter the following solutions from anaerobic stocks (autoclaved and stored under a N2 atmosphere):
  3. Readjust pH to 7.2, if necessary.

Solutions

Trace elements solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeSO4 x 7 H2O 5.55 G_PER_L
CoCl2 x 6 H2O 1 G_PER_L
MnSO4 x 5 H2O 1 G_PER_L
Na2MoO4 x 2 H2O 1 G_PER_L
Na2WO4 x 2 H2O 1 G_PER_L
ZnSO4 x 7 H2O 1 G_PER_L
NiCl2 x 6 H2O 3 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.656550Z mediadive-import Imported from MediaDive Source: JCM, ID: J1107
2026-03-13T06:19:11.983090Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002284
2026-03-17T06:29:54.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:24.878982+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LIQUID BASAL MEDIUM FOR METHANOSARCINA" to "liquid_basal_medium_for_methanosarcina" for consistent matching
2026-06-05T07:41:11.219149+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.564447+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-13T23:31:54.237085+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 7 stock-strength component(s) out of ingredients into 1 solution(s): 7 -> 'Trace elements solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).