natronospira_proteinivora_medium

Overview

External ontology

mediadive.medium:J1139 (source link unavailable) — NATRONOSPIRA PROTEINIVORA MEDIUM

Applications

• Microbial cultivation

pH

6.8

High-metal flag

True

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1139

Composition

flowchart LR
    CultureMech_002312["natronospira_proteinivora_medium"]
    ing0_Tryptone["Tryptone"]
    CultureMech_002312 --> ing0_Tryptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002312 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_002312 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_K2HPO4["K2HPO4"]
    CultureMech_002312 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002312 --> ing4__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing4__NH4_2SO4 -.-> CHEBI_62946
    ing5_MgCl2["MgCl2"]
    CultureMech_002312 --> ing5_MgCl2
    CHEBI_6636["CHEBI:6636"]
    ing5_MgCl2 -.-> CHEBI_6636
    ing6_Na2CO3["Na2CO3"]
    CultureMech_002312 --> ing6_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing6_Na2CO3 -.-> CHEBI_29377
    ing7_NaHCO3["NaHCO3"]
    CultureMech_002312 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139
    ing8_NH4Cl["NH4Cl"]
    CultureMech_002312 --> ing8_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing8_NH4Cl -.-> CHEBI_31206
    ing9_Casein["Casein"]
    CultureMech_002312 --> ing9_Casein
    ing10_EDTA["EDTA"]
    CultureMech_002312 --> ing10_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing10_EDTA -.-> CHEBI_4735
    ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002312 --> ing11_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002312 --> ing12_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002312 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_H3BO3["H3BO3"]
    CultureMech_002312 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002312 --> ing15_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002312 --> ing16_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002312 --> ing17_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002312 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (19)

Preparation

1. Aseptically combine the above described media and solutions.
2. Add components to distilled water and bring volume to 1.0 L. Adjust pH to 6.8 - 6.9 by 1 M K2HPO4 and autoclave. After cooling, add the following solutions (autoclaved):
3. Add components to distilled water and bring volume to 1.0 L. Check pH of the medium to be 9.9 - 10.0. Autoclave and let it stand for three days. Decant the clear solution into another empty sterile bottle. Aseptically add per liter the following solutions (autoclaved):
4. Suspend casein in distilled water and keep at 50C. Titrate pH to 8.2 - 8.5 with 1 M NaOH solution, until pH is stabilized. Autoclave the solution at 120C for 40 min in a closed bottle.
5. Adjust pH to 3.6.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• natronospira_proteinivora_medium (CultureMech:002312)