natronospira_proteinivora_medium

CultureMech:002312 bacterial COMPLEX LIQUID
Original name: NATRONOSPIRA PROTEINIVORA MEDIUM

Overview

External ontology
mediadive.medium:J1139 (source link unavailable) — NATRONOSPIRA PROTEINIVORA MEDIUM
Applications
  • Microbial cultivation
pH
6.8
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1139

Composition

flowchart LR
    CultureMech_002312["natronospira_proteinivora_medium"]
    ing0_Tryptone["Tryptone"]
    CultureMech_002312 --> ing0_Tryptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002312 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_002312 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_K2HPO4["K2HPO4"]
    CultureMech_002312 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002312 --> ing4__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing4__NH4_2SO4 -.-> CHEBI_62946
    ing5_MgCl2["MgCl2"]
    CultureMech_002312 --> ing5_MgCl2
    CHEBI_6636["CHEBI:6636"]
    ing5_MgCl2 -.-> CHEBI_6636
    ing6_Na2CO3["Na2CO3"]
    CultureMech_002312 --> ing6_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing6_Na2CO3 -.-> CHEBI_29377
    ing7_NaHCO3["NaHCO3"]
    CultureMech_002312 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139
    ing8_NH4Cl["NH4Cl"]
    CultureMech_002312 --> ing8_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing8_NH4Cl -.-> CHEBI_31206
    ing9_Casein["Casein"]
    CultureMech_002312 --> ing9_Casein
    ing10_EDTA["EDTA"]
    CultureMech_002312 --> ing10_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing10_EDTA -.-> CHEBI_4735
    ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002312 --> ing11_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002312 --> ing12_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002312 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_H3BO3["H3BO3"]
    CultureMech_002312 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002312 --> ing15_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002312 --> ing16_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002312 --> ing17_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002312 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (19)

# Ingredient Concentration Ontology Notes
1 Tryptone 15 G_PER_L
2 Yeast extract 2 G_PER_L
3 NaCl
Cl.Na 58.443 g/mol
122285.71 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 120000.0, 2285.71]
4 K2HPO4
HO4P.2K 174.174 g/mol
1535.714 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 [Merged 2 duplicates: 1250.0, 285.714]
5 (NH4)2SO4
2H4N.O4S 132.141 g/mol
250 G_PER_L CHEBI:62946
ammonium sulfate
6 MgCl2
2Cl.Mg 95.211 g/mol
1.0 G_PER_L CHEBI:6636
magnesium dichloride
 [Collapsed 2 identical duplicates: 1.0, 1.0]
7 Na2CO3
CO3.2Na 105.988 g/mol
27142.9 G_PER_L CHEBI:29377
sodium carbonate
8 NaHCO3
CHO3.Na 84.006 g/mol
4285.71 G_PER_L CHEBI:32139
sodium hydrogencarbonate
9 NH4Cl
Cl.H4N 53.492 g/mol
4 G_PER_L CHEBI:31206
ammonium chloride
10 Casein 50 G_PER_L
11 EDTA
C10H16N2O8 292.244 g/mol
5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
12 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
13 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
14 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
15 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
16 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
17 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
18 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
19 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Aseptically combine the above described media and solutions.
  2. Add components to distilled water and bring volume to 1.0 L. Adjust pH to 6.8 - 6.9 by 1 M K2HPO4 and autoclave. After cooling, add the following solutions (autoclaved):
  3. Add components to distilled water and bring volume to 1.0 L. Check pH of the medium to be 9.9 - 10.0. Autoclave and let it stand for three days. Decant the clear solution into another empty sterile bottle. Aseptically add per liter the following solutions (autoclaved):
  4. Suspend casein in distilled water and keep at 50C. Titrate pH to 8.2 - 8.5 with 1 M NaOH solution, until pH is stabilized. Autoclave the solution at 120C for 40 min in a closed bottle.
  5. Adjust pH to 3.6.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-14T05:58:58.561437+00:00 idnf-grounding-fix-v1.0 Fixed ID_NOT_FOUND term groundings Absent/obsolete CHEBI term ids fixed - 0 re-grounded to the correct CHEBI, 1 de-grounded (mixture or unverifiable).
2026-01-26T17:53:18.772521Z mediadive-import Imported from MediaDive Source: JCM, ID: J1139
2026-03-13T03:08:40.547682Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:19:12.243084Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002312
2026-03-17T06:29:42.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:25.183495+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NATRONOSPIRA PROTEINIVORA MEDIUM" to "natronospira_proteinivora_medium" for consistent matching
2026-06-05T07:41:39.416540+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.457992+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-02T07:38:53.651735+00:00 repair_merged_duplicates.py REPAIRED_SUMMED_DUPLICATE_MERGE Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched.