halanaerobium_hydrogeniformans_medium

CultureMech:002377 bacterial COMPLEX LIQUID
Original name: HALANAEROBIUM HYDROGENIFORMANS MEDIUM

Overview

External ontology
mediadive.medium:J1209 (source link unavailable) — HALANAEROBIUM HYDROGENIFORMANS MEDIUM
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1209

Composition

flowchart LR
    CultureMech_002377["halanaerobium_hydrogeniformans_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002377 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_Na2CO3["Na2CO3"]
    CultureMech_002377 --> ing1_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing1_Na2CO3 -.-> CHEBI_29377
    ing2_K2HPO4["K2HPO4"]
    CultureMech_002377 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_002377 --> ing3_Yeast_extract
    ing4_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002377 --> ing4_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing4_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing5_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002377 --> ing5_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing5_Na2S_x_9_H2O -.-> CHEBI_76209
    ing6_NH4NO3["NH4NO3"]
    CultureMech_002377 --> ing6_NH4NO3
    CHEBI_63038["CHEBI:63038"]
    ing6_NH4NO3 -.-> CHEBI_63038
    ing7_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002377 --> ing7_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing7_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing8_SiO2["SiO2"]
    CultureMech_002377 --> ing8_SiO2
    CHEBI_30563["CHEBI:30563"]
    ing8_SiO2 -.-> CHEBI_30563
    ing9_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002377 --> ing9_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing9_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002377 --> ing10_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing11_Methylene_blue["Methylene blue"]
    CultureMech_002377 --> ing11_Methylene_blue
    CHEBI_6872["CHEBI:6872"]
    ing11_Methylene_blue -.-> CHEBI_6872
    ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002377 --> ing12_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing13_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002377 --> ing13_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing13_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing14_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002377 --> ing14_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing14_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing15_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002377 --> ing15_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing15_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002377 --> ing16_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing17_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002377 --> ing17_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing17_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing18_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_002377 --> ing18_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing18_AlK_SO4_2 -.-> CHEBI_86463
    ing19_H3BO3["H3BO3"]
    CultureMech_002377 --> ing19_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing19_H3BO3 -.-> CHEBI_33118
    ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002377 --> ing20_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (21)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
68.9612 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 67.9612, 1.0]
2 Na2CO3
CO3.2Na 105.988 g/mol
38.835 G_PER_L CHEBI:29377
sodium carbonate
3 K2HPO4
HO4P.2K 174.174 g/mol
6.1165 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 Yeast extract 10 G_PER_L
5 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
10 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
6 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
10 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
7 NH4NO3
H4N.NO3 80.043 g/mol
0.05 G_PER_L CHEBI:63038
ammonium nitrate
8 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.0085 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
9 SiO2
O2Si 60.084 g/mol
0.0075 G_PER_L CHEBI:30563
silicon dioxide
10 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5045 G_PER_L CHEBI:86360
manganese(II) sulfate
 [Merged 2 duplicates: 0.0045, 0.5]
11 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1042 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.0042, 0.1]
12 Methylene blue
C16H18N3S.Cl 319.861 g/mol
0.004 G_PER_L CHEBI:6872
methylene blue
13 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1018 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 [Merged 2 duplicates: 0.0018, 0.1]
14 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
15 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
16 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
17 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
18 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
19 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
20 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
21 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Solution A:
  2. Solution B:
  3. Autoclave solutions A and B, separately, under a N2 gas atmosphere. After cooling, combine both solutions and add the following solution (autoclaved and stored under N2):
  4. Aseptically and anaerobically, distribute the medium into culture vessels (e.g., 10 ml in Balch type tubes) under a N2 gas stream and seal with butyl rubber stoppers. Finally, add per liter the following solutions (autoclaved and stored under N2):
  5. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Solutions

Basal salts solution

Dose: 10 G_PER_L

See mediadive_4011_Basal_salts_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:19.026187Z mediadive-import Imported from MediaDive Source: JCM, ID: J1209
2026-01-28T19:48:24.542498Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:40.571592+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T03:09:10.554022Z data-quality-cleanup-v1.0 Data quality fixes Merged 4 duplicate ingredient(s)
2026-03-13T06:19:12.841392Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002377
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:17.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:25.883923+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALANAEROBIUM HYDROGENIFORMANS MEDIUM" to "halanaerobium_hydrogeniformans_medium" for consistent matching
2026-05-16T09:03:36.231211+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:07.063149+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.285738+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:36.865588+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 4da99b2dd10f8cac2a0991c9f6ee108afc02c46c0a8890646a1dbccfc5d6f1bd