Overview
- External ontology
- mediadive.medium:J1209 (source link unavailable) — HALANAEROBIUM HYDROGENIFORMANS MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1209
Composition
flowchart LR
CultureMech_002377["halanaerobium_hydrogeniformans_medium"]
ing0_NaCl["NaCl"]
CultureMech_002377 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_Na2CO3["Na2CO3"]
CultureMech_002377 --> ing1_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing1_Na2CO3 -.-> CHEBI_29377
ing2_K2HPO4["K2HPO4"]
CultureMech_002377 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_Yeast_extract["Yeast extract"]
CultureMech_002377 --> ing3_Yeast_extract
ing4_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002377 --> ing4_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing4_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing5_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002377 --> ing5_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing5_Na2S_x_9_H2O -.-> CHEBI_76209
ing6_NH4NO3["NH4NO3"]
CultureMech_002377 --> ing6_NH4NO3
CHEBI_63038["CHEBI:63038"]
ing6_NH4NO3 -.-> CHEBI_63038
ing7_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002377 --> ing7_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_x_6_H2O -.-> CHEBI_86345
ing8_SiO2["SiO2"]
CultureMech_002377 --> ing8_SiO2
CHEBI_30563["CHEBI:30563"]
ing8_SiO2 -.-> CHEBI_30563
ing9_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002377 --> ing9_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing9_MnSO4_x_n_H2O -.-> CHEBI_86360
ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002377 --> ing10_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
ing11_Methylene_blue["Methylene blue"]
CultureMech_002377 --> ing11_Methylene_blue
CHEBI_6872["CHEBI:6872"]
ing11_Methylene_blue -.-> CHEBI_6872
ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002377 --> ing12_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
ing13_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002377 --> ing13_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing13_Nitrilotriacetic_acid -.-> CHEBI_44557
ing14_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002377 --> ing14_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing14_MgSO4_x_7_H2O -.-> CHEBI_31795
ing15_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002377 --> ing15_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing15_CoSO4_x_7_H2O -.-> CHEBI_91244
ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002377 --> ing16_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing17_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002377 --> ing17_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing17_CuSO4_x_5_H2O -.-> CHEBI_31440
ing18_AlK_SO4_2["AlK(SO4)2"]
CultureMech_002377 --> ing18_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing18_AlK_SO4_2 -.-> CHEBI_86463
ing19_H3BO3["H3BO3"]
CultureMech_002377 --> ing19_H3BO3
CHEBI_33118["CHEBI:33118"]
ing19_H3BO3 -.-> CHEBI_33118
ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002377 --> ing20_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
68.9612 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 67.9612, 1.0] |
| 2 |
Na2CO3
CO3.2Na 105.988 g/mol
|
38.835 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
6.1165 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 | Yeast extract | 10 G_PER_L | ||
| 5 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
10 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 6 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
10 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 7 |
NH4NO3
H4N.NO3 80.043 g/mol
|
0.05 G_PER_L |
CHEBI:63038
ammonium nitrate
|
|
| 8 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.0085 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 9 |
SiO2
O2Si 60.084 g/mol
|
0.0075 G_PER_L |
CHEBI:30563
silicon dioxide
|
|
| 10 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5045 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
[Merged 2 duplicates: 0.0045, 0.5] |
| 11 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1042 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.0042, 0.1] |
| 12 |
Methylene blue
C16H18N3S.Cl 319.861 g/mol
|
0.004 G_PER_L |
CHEBI:6872
methylene blue
|
|
| 13 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1018 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 0.0018, 0.1] |
| 14 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 15 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 16 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 17 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 18 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 19 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 20 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 21 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Solution A:
- Solution B:
- Autoclave solutions A and B, separately, under a N2 gas atmosphere. After cooling, combine both solutions and add the following solution (autoclaved and stored under N2):
- Aseptically and anaerobically, distribute the medium into culture vessels (e.g., 10 ml in Balch type tubes) under a N2 gas stream and seal with butyl rubber stoppers. Finally, add per liter the following solutions (autoclaved and stored under N2):
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Solutions
Basal salts solution
Dose: 10 G_PER_L
See mediadive_4011_Basal_salts_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.026187Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1209 |
| 2026-01-28T19:48:24.542498Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:40.571592+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:10.554022Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:12.841392Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002377 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:17.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:25.883923+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALANAEROBIUM HYDROGENIFORMANS MEDIUM" to "halanaerobium_hydrogeniformans_medium" for consistent matching |
| 2026-05-16T09:03:36.231211+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:07.063149+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.285738+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |