Overview
- External ontology
- mediadive.medium:J1214 (source link unavailable) — RECTINEMA SUBTERRANEUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1214
Composition
flowchart LR
CultureMech_002383["rectinema_subterraneum_medium"]
ing0_NaCl["NaCl"]
CultureMech_002383 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_Peptone["Peptone"]
CultureMech_002383 --> ing1_Peptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_002383 --> ing2_Yeast_extract
ing3_HEPES["HEPES"]
CultureMech_002383 --> ing3_HEPES
CHEBI_46756["CHEBI:46756"]
ing3_HEPES -.-> CHEBI_46756
ing4_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002383 --> ing4_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing4_Na2S_x_9_H2O -.-> CHEBI_76209
ing5_KCl["KCl"]
CultureMech_002383 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002383 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
CultureMech_002383 --> ing7_CaSO4_x_2_H2O
CHEBI_32583["CHEBI:32583"]
ing7_CaSO4_x_2_H2O -.-> CHEBI_32583
ing8_Na2SO4["Na2SO4"]
CultureMech_002383 --> ing8_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing8_Na2SO4 -.-> CHEBI_32149
ing9_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002383 --> ing9_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing9_CaCl2_x_2_H2O -.-> CHEBI_86158
ing10_NH4Cl["NH4Cl"]
CultureMech_002383 --> ing10_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing10_NH4Cl -.-> CHEBI_31206
ing11__NH4_2SO4["(NH4)2SO4"]
CultureMech_002383 --> ing11__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing11__NH4_2SO4 -.-> CHEBI_62946
ing12_KH2PO4["KH2PO4"]
CultureMech_002383 --> ing12_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing12_KH2PO4 -.-> CHEBI_63036
ing13_NaF["NaF"]
CultureMech_002383 --> ing13_NaF
CHEBI_28741["CHEBI:28741"]
ing13_NaF -.-> CHEBI_28741
ing14_LiCl["LiCl"]
CultureMech_002383 --> ing14_LiCl
CHEBI_48607["CHEBI:48607"]
ing14_LiCl -.-> CHEBI_48607
ing15_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002383 --> ing15_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing15_Nitrilotriacetic_acid -.-> CHEBI_44557
ing16_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002383 --> ing16_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing16_MnSO4_x_n_H2O -.-> CHEBI_86360
ing17_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002383 --> ing17_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing17_FeSO4_x_7_H2O -.-> CHEBI_75836
ing18_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002383 --> ing18_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing18_CoSO4_x_7_H2O -.-> CHEBI_91244
ing19_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002383 --> ing19_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing19_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing20_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002383 --> ing20_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing20_CuSO4_x_5_H2O -.-> CHEBI_31440
ing21_AlK_SO4_2["AlK(SO4)2"]
CultureMech_002383 --> ing21_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing21_AlK_SO4_2 -.-> CHEBI_86463
ing22_H3BO3["H3BO3"]
CultureMech_002383 --> ing22_H3BO3
CHEBI_33118["CHEBI:33118"]
ing22_H3BO3 -.-> CHEBI_33118
ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002383 --> ing23_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing24_Biotin["Biotin"]
CultureMech_002383 --> ing24_Biotin
CHEBI_15956["CHEBI:15956"]
ing24_Biotin -.-> CHEBI_15956
ing25_Folic_acid["Folic acid"]
CultureMech_002383 --> ing25_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing25_Folic_acid -.-> CHEBI_27470
ing26_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002383 --> ing26_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing26_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing27_Thiamine_HCl["Thiamine HCl"]
CultureMech_002383 --> ing27_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing27_Thiamine_HCl -.-> CHEBI_49105
ing28_Riboflavin["Riboflavin"]
CultureMech_002383 --> ing28_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing28_Riboflavin -.-> CHEBI_17015
ing29_Nicotinic_acid["Nicotinic acid"]
CultureMech_002383 --> ing29_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing29_Nicotinic_acid -.-> CHEBI_15940
CultureMech_002383 --> more["...4 more"]
Ingredients (34)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
10.90099 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 9.90099, 1.0] |
| 2 | Peptone | 0.990099 G_PER_L | ||
| 3 | Yeast extract | 0.990099 G_PER_L | ||
| 4 | HEPES | 0.990099 G_PER_L |
CHEBI:46756
HEPES
|
|
| 5 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
10 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.12 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 0.3, 3.0] |
| 8 |
CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.076 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
|
| 9 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.4 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 10 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.124 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.024, 0.1] |
| 11 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 12 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.32 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 13 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.45 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 14 |
NaF
F.Na 41.988 g/mol
|
0.06 G_PER_L |
CHEBI:28741
sodium fluoride
|
|
| 15 |
LiCl
Cl.Li 42.394 g/mol
|
0.02 G_PER_L |
CHEBI:48607
lithium chloride
|
|
| 16 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 17 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 18 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 19 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 20 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 21 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 22 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 23 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 24 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 25 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 26 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 27 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 28 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 29 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 30 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 31 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 32 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 33 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 34 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix components thoroughly, adjust pH to 7.2 and autoclave under a H2-CO2 (80:20, v/v) gas mixture. After cooling, aseptically and anaerobically add the following solution (autoclaved and stored under N2):
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- rectinema_subterraneum_medium (CultureMech:002383)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.047065Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1214 |
| 2026-03-13T03:09:22.953554Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:12.894661Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002383 |
| 2026-03-17T06:30:31.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:25.941609+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RECTINEMA SUBTERRANEUM MEDIUM" to "rectinema_subterraneum_medium" for consistent matching |
| 2026-06-05T07:41:44.134888+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.886579+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 7 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:54.540751+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint fb4b2674a3072592c6057254a904a898871a1fa9bd6929affba4c6dc6e8b7ca4 |