Overview
- External ontology
- mediadive.medium:J1214 (source link unavailable) — RECTINEMA SUBTERRANEUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1214
Composition
flowchart LR
CultureMech_002383["rectinema_subterraneum_medium"]
ing0_NaCl["NaCl"]
CultureMech_002383 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_Peptone["Peptone"]
CultureMech_002383 --> ing1_Peptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_002383 --> ing2_Yeast_extract
ing3_HEPES["HEPES"]
CultureMech_002383 --> ing3_HEPES
CHEBI_46756["CHEBI:46756"]
ing3_HEPES -.-> CHEBI_46756
ing4_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002383 --> ing4_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing4_Na2S_x_9_H2O -.-> CHEBI_76209
ing5_KCl["KCl"]
CultureMech_002383 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002383 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
CultureMech_002383 --> ing7_CaSO4_x_2_H2O
CHEBI_32583["CHEBI:32583"]
ing7_CaSO4_x_2_H2O -.-> CHEBI_32583
ing8_Na2SO4["Na2SO4"]
CultureMech_002383 --> ing8_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing8_Na2SO4 -.-> CHEBI_32149
ing9_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002383 --> ing9_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing9_CaCl2_x_2_H2O -.-> CHEBI_86158
ing10_NH4Cl["NH4Cl"]
CultureMech_002383 --> ing10_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing10_NH4Cl -.-> CHEBI_31206
ing11__NH4_2SO4["(NH4)2SO4"]
CultureMech_002383 --> ing11__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing11__NH4_2SO4 -.-> CHEBI_62946
ing12_KH2PO4["KH2PO4"]
CultureMech_002383 --> ing12_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing12_KH2PO4 -.-> CHEBI_63036
ing13_NaF["NaF"]
CultureMech_002383 --> ing13_NaF
CHEBI_28741["CHEBI:28741"]
ing13_NaF -.-> CHEBI_28741
ing14_LiCl["LiCl"]
CultureMech_002383 --> ing14_LiCl
CHEBI_48607["CHEBI:48607"]
ing14_LiCl -.-> CHEBI_48607
ing15_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002383 --> ing15_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing15_Nitrilotriacetic_acid -.-> CHEBI_44557
ing16_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002383 --> ing16_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing16_MnSO4_x_n_H2O -.-> CHEBI_86360
ing17_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002383 --> ing17_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing17_FeSO4_x_7_H2O -.-> CHEBI_75836
ing18_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002383 --> ing18_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing18_CoSO4_x_7_H2O -.-> CHEBI_91244
ing19_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002383 --> ing19_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing19_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing20_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002383 --> ing20_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing20_CuSO4_x_5_H2O -.-> CHEBI_31440
ing21_AlK_SO4_2["AlK(SO4)2"]
CultureMech_002383 --> ing21_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing21_AlK_SO4_2 -.-> CHEBI_86463
ing22_H3BO3["H3BO3"]
CultureMech_002383 --> ing22_H3BO3
CHEBI_33118["CHEBI:33118"]
ing22_H3BO3 -.-> CHEBI_33118
ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002383 --> ing23_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing24_Biotin["Biotin"]
CultureMech_002383 --> ing24_Biotin
CHEBI_15956["CHEBI:15956"]
ing24_Biotin -.-> CHEBI_15956
ing25_Folic_acid["Folic acid"]
CultureMech_002383 --> ing25_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing25_Folic_acid -.-> CHEBI_27470
ing26_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002383 --> ing26_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing26_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing27_Thiamine_HCl["Thiamine HCl"]
CultureMech_002383 --> ing27_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing27_Thiamine_HCl -.-> CHEBI_49105
ing28_Riboflavin["Riboflavin"]
CultureMech_002383 --> ing28_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing28_Riboflavin -.-> CHEBI_17015
ing29_Nicotinic_acid["Nicotinic acid"]
CultureMech_002383 --> ing29_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing29_Nicotinic_acid -.-> CHEBI_15940
CultureMech_002383 --> more["...4 more"]
Ingredients (34)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
10.90099 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 9.90099, 1.0] |
| 2 | Peptone | 0.990099 G_PER_L | ||
| 3 | Yeast extract | 0.990099 G_PER_L | ||
| 4 | HEPES | 0.990099 G_PER_L |
CHEBI:46756
HEPES
|
|
| 5 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
10 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.12 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 0.3, 3.0] |
| 8 |
CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.076 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
|
| 9 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.4 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 10 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.124 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.024, 0.1] |
| 11 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 12 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.32 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 13 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.45 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 14 |
NaF
F.Na 41.988 g/mol
|
0.06 G_PER_L |
CHEBI:28741
sodium fluoride
|
|
| 15 |
LiCl
Cl.Li 42.394 g/mol
|
0.02 G_PER_L |
CHEBI:48607
lithium chloride
|
|
| 16 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 17 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 18 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 19 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 20 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 21 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 22 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 23 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 24 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 25 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 26 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 27 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 28 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 29 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 30 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 31 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 32 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 33 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 34 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix components thoroughly, adjust pH to 7.2 and autoclave under a H2-CO2 (80:20, v/v) gas mixture. After cooling, aseptically and anaerobically add the following solution (autoclaved and stored under N2):
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.047065Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1214 |
| 2026-03-13T03:09:22.953554Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:12.894661Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002383 |
| 2026-03-17T06:30:31.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:25.941609+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RECTINEMA SUBTERRANEUM MEDIUM" to "rectinema_subterraneum_medium" for consistent matching |
| 2026-06-05T07:41:44.134888+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.886579+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 7 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |