hydrogenobacter_florentissimum_medium

Overview

External ontology

mediadive.medium:J1255 (source link unavailable) — HYDROGENOBACTER FLORENTISSIMUM MEDIUM

Applications

• Microbial cultivation

pH

6.7

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1255

Composition

flowchart LR
    CultureMech_002421["hydrogenobacter_florentissimum_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002421 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_KCl["KCl"]
    CultureMech_002421 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_Na2SO4["Na2SO4"]
    CultureMech_002421 --> ing2_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing2_Na2SO4 -.-> CHEBI_32149
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002421 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002421 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_NH4Cl["NH4Cl"]
    CultureMech_002421 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_NaH2PO4["NaH2PO4"]
    CultureMech_002421 --> ing6_NaH2PO4
    CHEBI_37585["CHEBI:37585"]
    ing6_NaH2PO4 -.-> CHEBI_37585
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_002421 --> ing7_Yeast_extract
    ing8_Sodium_Thiosulfate["Sodium Thiosulfate"]
    CultureMech_002421 --> ing8_Sodium_Thiosulfate
    CHEBI_132112["CHEBI:132112"]
    ing8_Sodium_Thiosulfate -.-> CHEBI_132112
    ing9_EDTA["EDTA"]
    CultureMech_002421 --> ing9_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing9_EDTA -.-> CHEBI_4735
    ing10_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002421 --> ing10_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing10_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002421 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002421 --> ing12_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002421 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002421 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002421 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing16_H3BO3["H3BO3"]
    CultureMech_002421 --> ing16_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing16_H3BO3 -.-> CHEBI_33118

Ingredients (17)

Preparation

1. Mix components thoroughly and adjust pH to 6.75 with NaOH. Distribute the medium into culture vessels, leaving &; 80% volume of gas phase (e.g., 5.0 ml in 25 ml serum bottles or Balch tubes), under a stream of N2, seal with butyl rubber stoppers and autoclave. After cooling, aseptically add per liter the following solutions (autoclaved):
2. Then, add filter-sterilized H2, CO2 and air to the culture vessels to make the gaseous composition in the gas phase 75% N2, 17% H2, 4% CO2 and 4% air by volume.
3. Adjust pH to 6.0 with KOH.
4. Comment: Consistent growth in serum tubes requires pretreatment of butyl rubber stoppers by boiling in 1% sodium sulfide solution (1 hr) followed by boiling in distilled H2O (1 hr, twice).

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• hydrogenobacter_florentissimum_medium (CultureMech:002421)