hydrogenobacter_florentissimum_medium

CultureMech:002421 bacterial COMPLEX LIQUID
Original name: HYDROGENOBACTER FLORENTISSIMUM MEDIUM

Overview

External ontology
mediadive.medium:J1255 (source link unavailable) — HYDROGENOBACTER FLORENTISSIMUM MEDIUM
Applications
  • Microbial cultivation
pH
6.7
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1255

Composition

flowchart LR
    CultureMech_002421["hydrogenobacter_florentissimum_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002421 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_KCl["KCl"]
    CultureMech_002421 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_Na2SO4["Na2SO4"]
    CultureMech_002421 --> ing2_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing2_Na2SO4 -.-> CHEBI_32149
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002421 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002421 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_NH4Cl["NH4Cl"]
    CultureMech_002421 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_NaH2PO4["NaH2PO4"]
    CultureMech_002421 --> ing6_NaH2PO4
    CHEBI_37585["CHEBI:37585"]
    ing6_NaH2PO4 -.-> CHEBI_37585
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_002421 --> ing7_Yeast_extract
    ing8_Sodium_Thiosulfate["Sodium Thiosulfate"]
    CultureMech_002421 --> ing8_Sodium_Thiosulfate
    CHEBI_132112["CHEBI:132112"]
    ing8_Sodium_Thiosulfate -.-> CHEBI_132112
    ing9_EDTA["EDTA"]
    CultureMech_002421 --> ing9_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing9_EDTA -.-> CHEBI_4735
    ing10_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002421 --> ing10_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing10_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002421 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002421 --> ing12_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002421 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002421 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002421 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing16_H3BO3["H3BO3"]
    CultureMech_002421 --> ing16_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing16_H3BO3 -.-> CHEBI_33118

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
2.94118 G_PER_L CHEBI:26710
sodium chloride
2 KCl
Cl.K 74.551 g/mol
0.147059 G_PER_L CHEBI:32588
potassium chloride
3 Na2SO4
2Na.O4S 142.043 g/mol
0.294118 G_PER_L CHEBI:32149
sodium sulfate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.120588 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0147059 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 NH4Cl
Cl.H4N 53.492 g/mol
0.104902 G_PER_L CHEBI:31206
ammonium chloride
7 NaH2PO4
H2O4P.Na 119.976 g/mol
1.35294 G_PER_L CHEBI:37585
sodium dihydrogenphosphate
8 Yeast extract 10 G_PER_L
9 Sodium Thiosulfate
2Na.O3S2 158.111 g/mol
5 G_PER_L CHEBI:132112
sodium thiosulfate
10 EDTA
C10H16N2O8 292.244 g/mol
3.7 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
11 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
1.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
12 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.0984 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
13 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.0288 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
14 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.0238 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
15 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.0172 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
16 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.0242 G_PER_L CHEBI:75213
sodium molybdate dihydrate
17 H3BO3
H3BO3 61.833 g/mol
0.031 G_PER_L CHEBI:33118
boric acid

Preparation

  1. Mix components thoroughly and adjust pH to 6.75 with NaOH. Distribute the medium into culture vessels, leaving &; 80% volume of gas phase (e.g., 5.0 ml in 25 ml serum bottles or Balch tubes), under a stream of N2, seal with butyl rubber stoppers and autoclave. After cooling, aseptically add per liter the following solutions (autoclaved):
  2. Then, add filter-sterilized H2, CO2 and air to the culture vessels to make the gaseous composition in the gas phase 75% N2, 17% H2, 4% CO2 and 4% air by volume.
  3. Adjust pH to 6.0 with KOH.
  4. Comment: Consistent growth in serum tubes requires pretreatment of butyl rubber stoppers by boiling in 1% sodium sulfide solution (1 hr) followed by boiling in distilled H2O (1 hr, twice).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.188134Z mediadive-import Imported from MediaDive Source: JCM, ID: J1255
2026-03-13T06:19:13.174535Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002421
2026-03-17T06:29:41.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:26.254102+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HYDROGENOBACTER FLORENTISSIMUM MEDIUM" to "hydrogenobacter_florentissimum_medium" for consistent matching
2026-06-05T07:41:09.014343+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.918154+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.