Overview
- External ontology
- mediadive.medium:J1267 (source link unavailable) — MODIFIED PICROPHILUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 2.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1267
Composition
flowchart LR
CultureMech_002433["modified_picrophilus_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_002433 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002433 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002433 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_Tryptone["Tryptone"]
CultureMech_002433 --> ing3_Tryptone
ing4_Yeast_extract["Yeast extract"]
CultureMech_002433 --> ing4_Yeast_extract
ing5_Fe2_SO4_3["Fe2(SO4)3"]
CultureMech_002433 --> ing5_Fe2_SO4_3
ing6_Vitamin_B12["Vitamin B12"]
CultureMech_002433 --> ing6_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing6_Vitamin_B12 -.-> CHEBI_176843
ing7_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
CultureMech_002433 --> ing7_DL_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing7_DL_Calcium_pantothenate -.-> CHEBI_31345
ing8_Riboflavin["Riboflavin"]
CultureMech_002433 --> ing8_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing8_Riboflavin -.-> CHEBI_17015
ing9_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002433 --> ing9_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing9_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing10_Biotin["Biotin"]
CultureMech_002433 --> ing10_Biotin
CHEBI_15956["CHEBI:15956"]
ing10_Biotin -.-> CHEBI_15956
ing11_Folic_acid["Folic acid"]
CultureMech_002433 --> ing11_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing11_Folic_acid -.-> CHEBI_27470
ing12_Nicotinic_acid["Nicotinic acid"]
CultureMech_002433 --> ing12_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing12_Nicotinic_acid -.-> CHEBI_15940
ing13_Nicotine_amide["Nicotine amide"]
CultureMech_002433 --> ing13_Nicotine_amide
CHEBI_17154["CHEBI:17154"]
ing13_Nicotine_amide -.-> CHEBI_17154
ing14_Lipoic_acid["Lipoic acid"]
CultureMech_002433 --> ing14_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing14_Lipoic_acid -.-> CHEBI_16494
ing15_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002433 --> ing15_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing15_p_Aminobenzoic_acid -.-> CHEBI_30753
ing16_Thiamine_HCl["Thiamine HCl"]
CultureMech_002433 --> ing16_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing16_Thiamine_HCl -.-> CHEBI_49105
ing17_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002433 --> ing17_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing17_MnSO4_x_n_H2O -.-> CHEBI_86360
ing18_NaCl["NaCl"]
CultureMech_002433 --> ing18_NaCl
CHEBI_26710["CHEBI:26710"]
ing18_NaCl -.-> CHEBI_26710
ing19_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002433 --> ing19_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing19_FeSO4_x_7_H2O -.-> CHEBI_75836
ing20_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002433 --> ing20_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing20_CoCl2_x_6_H2O -.-> CHEBI_53503
ing21_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002433 --> ing21_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing21_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing22_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002433 --> ing22_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing22_CuSO4_x_5_H2O -.-> CHEBI_31440
ing23_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_002433 --> ing23_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing23_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing24_H3BO3["H3BO3"]
CultureMech_002433 --> ing24_H3BO3
CHEBI_33118["CHEBI:33118"]
ing24_H3BO3 -.-> CHEBI_33118
ing25_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002433 --> ing25_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing25_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing26__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
CultureMech_002433 --> ing26__NH4_2Ni_SO4_2_x_6_H2O
CHEBI_86149["CHEBI:86149"]
ing26__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
ing27_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002433 --> ing27_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing27_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing28_Na2SeO4["Na2SeO4"]
CultureMech_002433 --> ing28_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing28_Na2SeO4 -.-> CHEBI_77775
Ingredients (29)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.179372 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
30.44843 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 0.44843, 30.0] |
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.224215 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.224215, 1.0] |
| 4 | Tryptone | 11.1 G_PER_L | ||
| 5 | Yeast extract | 11.1 G_PER_L | ||
| 6 | Fe2(SO4)3 | 88.8 G_PER_L | ||
| 7 | Vitamin B12 | 0.05 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 8 |
DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 9 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 10 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 11 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 12 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 13 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.025 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 14 |
Nicotine amide
C6H6N2O 122.127 g/mol
|
0.025 G_PER_L |
CHEBI:17154
nicotinamide
|
|
| 15 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 16 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 17 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 18 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 19 |
NaCl
Cl.Na 58.443 g/mol
|
10 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 20 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 21 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
1.8 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 22 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
1.8 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 23 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.1 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 24 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.18 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 25 |
H3BO3
H3BO3 61.833 g/mol
|
0.1 G_PER_L |
CHEBI:33118
boric acid
|
|
| 26 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 27 |
(NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
|
2.8 G_PER_L |
CHEBI:86149
ammonium nickel sulfate hexahydrate
|
|
| 28 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.1 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 29 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.1 G_PER_L |
CHEBI:77775
sodium selenate
|
Preparation
- Mix components thoroughly, adjust pH to 2.0 - 2.5 with H2SO4 and autoclave. After cooling, add the following solutions (filter-sterilized):
- Stir for several hours to dissolve.
- First adjust pH to 1.0 with diluted H2SO4, then dissolve the salts.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_picrophilus_medium (CultureMech:002433)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.232786Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1267 |
| 2026-03-13T03:09:02.932657Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:13.283650Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002433 |
| 2026-03-17T06:30:08.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.382258+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED PICROPHILUS MEDIUM" to "modified_picrophilus_medium" for consistent matching |
| 2026-06-05T07:41:37.616959+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.648836+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:08.906355+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:31.949980+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 2e13d4cdd9207a23f2613d4ab1cfc0221ca7657caa27bea30803d369481c6301 |