modified_picrophilus_medium

CultureMech:002433 archaea COMPLEX LIQUID
Original name: MODIFIED PICROPHILUS MEDIUM

Overview

External ontology
mediadive.medium:J1267 (source link unavailable) — MODIFIED PICROPHILUS MEDIUM
Applications
  • Microbial cultivation
pH
2.2
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1267

Composition

flowchart LR
    CultureMech_002433["modified_picrophilus_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002433 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002433 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002433 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_Tryptone["Tryptone"]
    CultureMech_002433 --> ing3_Tryptone
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_002433 --> ing4_Yeast_extract
    ing5_Fe2_SO4_3["Fe2(SO4)3"]
    CultureMech_002433 --> ing5_Fe2_SO4_3
    ing6_Vitamin_B12["Vitamin B12"]
    CultureMech_002433 --> ing6_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing6_Vitamin_B12 -.-> CHEBI_176843
    ing7_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
    CultureMech_002433 --> ing7_DL_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing7_DL_Calcium_pantothenate -.-> CHEBI_31345
    ing8_Riboflavin["Riboflavin"]
    CultureMech_002433 --> ing8_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing8_Riboflavin -.-> CHEBI_17015
    ing9_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002433 --> ing9_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing9_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing10_Biotin["Biotin"]
    CultureMech_002433 --> ing10_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing10_Biotin -.-> CHEBI_15956
    ing11_Folic_acid["Folic acid"]
    CultureMech_002433 --> ing11_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing11_Folic_acid -.-> CHEBI_27470
    ing12_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002433 --> ing12_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing12_Nicotinic_acid -.-> CHEBI_15940
    ing13_Nicotine_amide["Nicotine amide"]
    CultureMech_002433 --> ing13_Nicotine_amide
    CHEBI_17154["CHEBI:17154"]
    ing13_Nicotine_amide -.-> CHEBI_17154
    ing14_Lipoic_acid["Lipoic acid"]
    CultureMech_002433 --> ing14_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing14_Lipoic_acid -.-> CHEBI_16494
    ing15_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002433 --> ing15_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing15_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing16_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002433 --> ing16_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing16_Thiamine_HCl -.-> CHEBI_49105
    ing17_NaCl["NaCl"]
    CultureMech_002433 --> ing17_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing17_NaCl -.-> CHEBI_26710
    ing18_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002433 --> ing18_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing18_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing19_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_002433 --> ing19_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing19_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing20_H3BO3["H3BO3"]
    CultureMech_002433 --> ing20_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing20_H3BO3 -.-> CHEBI_33118
    ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002433 --> ing21_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing22__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
    CultureMech_002433 --> ing22__NH4_2Ni_SO4_2_x_6_H2O
    CHEBI_86149["CHEBI:86149"]
    ing22__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
    ing23_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002433 --> ing23_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing23_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing24_Na2SeO4["Na2SeO4"]
    CultureMech_002433 --> ing24_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing24_Na2SeO4 -.-> CHEBI_77775

Ingredients (25)

# Ingredient Concentration Ontology Notes
1 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.179372 G_PER_L CHEBI:62946
ammonium sulfate
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
30.44843 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 0.44843, 30.0]
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1.224215 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.224215, 1.0]
4 Tryptone 11.1 G_PER_L
5 Yeast extract 11.1 G_PER_L
6 Fe2(SO4)3 88.8 G_PER_L
7 Vitamin B12 0.05 G_PER_L CHEBI:176843
vitamin B12
8 DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
9 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
10 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
11 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
12 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
13 Nicotinic acid
C6H5NO2 123.111 g/mol
0.025 G_PER_L CHEBI:15940
nicotinic acid
14 Nicotine amide
C6H6N2O 122.127 g/mol
0.025 G_PER_L CHEBI:17154
nicotinamide
15 Lipoic acid
C8H14O2S2 206.332 g/mol
0.05 G_PER_L CHEBI:16494
lipoic acid
16 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
17 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.05 G_PER_L CHEBI:49105
thiamine hydrochloride
18 NaCl
Cl.Na 58.443 g/mol
10 G_PER_L CHEBI:26710
sodium chloride
19 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.1 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
20 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.18 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
21 H3BO3
H3BO3 61.833 g/mol
0.1 G_PER_L CHEBI:33118
boric acid
22 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.1 G_PER_L CHEBI:75213
sodium molybdate dihydrate
23 (NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
2.8 G_PER_L CHEBI:86149
ammonium nickel sulfate hexahydrate
24 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.1 G_PER_L CHEBI:63939
sodium tungstate dihydrate
25 Na2SeO4
2Na.O4Se 188.936 g/mol
0.1 G_PER_L CHEBI:77775
sodium selenate

Preparation

  1. Mix components thoroughly, adjust pH to 2.0 - 2.5 with H2SO4 and autoclave. After cooling, add the following solutions (filter-sterilized):
  2. Stir for several hours to dissolve.
  3. First adjust pH to 1.0 with diluted H2SO4, then dissolve the salts.

Solutions

Wolfe's mineral elixir

Dose: 2 ML_PER_L

Stock composition
IngredientConcentrationNotes
MnSO4 x n H2O 5 G_PER_L
FeSO4 x 7 H2O 1 G_PER_L
CoCl2 x 6 H2O 1.8 G_PER_L
ZnSO4 x 7 H2O 1.8 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.232786Z mediadive-import Imported from MediaDive Source: JCM, ID: J1267
2026-03-13T03:09:02.932657Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:13.283650Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002433
2026-03-17T06:30:08.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:26.382258+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MODIFIED PICROPHILUS MEDIUM" to "modified_picrophilus_medium" for consistent matching
2026-06-05T07:41:37.616959+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.648836+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:08.906355+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-13T23:31:54.254631+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 4 stock-strength component(s) out of ingredients into 1 solution(s): 4 -> "Wolfe's mineral elixir" @ 2 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).