Overview
- External ontology
- mediadive.medium:J1272 (source link unavailable) — ATRIBACTERIUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1272
Composition
flowchart LR
CultureMech_002438["atribacterium_medium"]
ing0_NaCl["NaCl"]
CultureMech_002438 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_KCl["KCl"]
CultureMech_002438 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_Na2SO4["Na2SO4"]
CultureMech_002438 --> ing2_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing2_Na2SO4 -.-> CHEBI_32149
ing3_NH4Cl["NH4Cl"]
CultureMech_002438 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_NaH2PO4["NaH2PO4"]
CultureMech_002438 --> ing4_NaH2PO4
CHEBI_37585["CHEBI:37585"]
ing4_NaH2PO4 -.-> CHEBI_37585
ing5_Xylitol["Xylitol"]
CultureMech_002438 --> ing5_Xylitol
CHEBI_17151["CHEBI:17151"]
ing5_Xylitol -.-> CHEBI_17151
ing6_Fucose["Fucose"]
CultureMech_002438 --> ing6_Fucose
CHEBI_33984["CHEBI:33984"]
ing6_Fucose -.-> CHEBI_33984
ing7_Yeast_extract["Yeast extract"]
CultureMech_002438 --> ing7_Yeast_extract
ing8_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002438 --> ing8_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing8_Na2S_x_9_H2O -.-> CHEBI_76209
ing9_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002438 --> ing9_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing9_MgSO4_x_7_H2O -.-> CHEBI_31795
ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002438 --> ing10_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
ing11_EDTA["EDTA"]
CultureMech_002438 --> ing11_EDTA
CHEBI_4735["CHEBI:4735"]
ing11_EDTA -.-> CHEBI_4735
ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002438 --> ing12_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002438 --> ing13_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
ing14_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002438 --> ing14_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing14_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002438 --> ing15_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002438 --> ing16_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002438 --> ing17_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing18_H3BO3["H3BO3"]
CultureMech_002438 --> ing18_H3BO3
CHEBI_33118["CHEBI:33118"]
ing18_H3BO3 -.-> CHEBI_33118
ing19_Biotin["Biotin"]
CultureMech_002438 --> ing19_Biotin
CHEBI_15956["CHEBI:15956"]
ing19_Biotin -.-> CHEBI_15956
ing20_Folic_acid["Folic acid"]
CultureMech_002438 --> ing20_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing20_Folic_acid -.-> CHEBI_27470
ing21_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002438 --> ing21_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing21_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing22_Thiamine_HCl["Thiamine HCl"]
CultureMech_002438 --> ing22_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing22_Thiamine_HCl -.-> CHEBI_49105
ing23_Riboflavin["Riboflavin"]
CultureMech_002438 --> ing23_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing23_Riboflavin -.-> CHEBI_17015
ing24_Nicotinic_acid["Nicotinic acid"]
CultureMech_002438 --> ing24_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing24_Nicotinic_acid -.-> CHEBI_15940
ing25_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002438 --> ing25_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing25_Calcium_pantothenate -.-> CHEBI_31345
ing26_Vitamin_B12["Vitamin B12"]
CultureMech_002438 --> ing26_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing26_Vitamin_B12 -.-> CHEBI_176843
ing27_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002438 --> ing27_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing27_p_Aminobenzoic_acid -.-> CHEBI_30753
ing28_Lipoic_acid["Lipoic acid"]
CultureMech_002438 --> ing28_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing28_Lipoic_acid -.-> CHEBI_16494
Ingredients (29)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
2.82486 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
0.141243 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.282486 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.100753 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
NaH2PO4
H2O4P.Na 119.976 g/mol
|
1.29944 G_PER_L |
CHEBI:37585
sodium dihydrogenphosphate
|
|
| 6 |
Xylitol
C5H12O5 152.146 g/mol
|
10 G_PER_L |
CHEBI:17151
xylitol
|
|
| 7 | Fucose | 10 G_PER_L |
CHEBI:33984
fucose
|
|
| 8 | Yeast extract | 10 G_PER_L | ||
| 9 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
2 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 10 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.115819 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 11 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0141243 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 12 |
EDTA
C10H16N2O8 292.244 g/mol
|
3.7 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 13 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 14 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.0984 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 15 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.0288 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 16 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.0238 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 17 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.0172 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 18 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.0242 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 19 |
H3BO3
H3BO3 61.833 g/mol
|
0.031 G_PER_L |
CHEBI:33118
boric acid
|
|
| 20 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 21 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 22 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 23 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 24 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 25 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 26 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 27 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 28 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 29 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix components thoroughly and adjust pH to 7.0 with NaOH. Distribute the medium into culture vessels (e.g., 10.0 ml in 25 ml serum bottles/Balch tubes) under a stream of N2, seal with butyl rubber stoppers and autoclave. After cooling, aseptically add per liter the following solutions (autoclaved or *filter-sterilized and stored anaerobically):
- Solution A:
- Adjust pH to 6.0 with KOH.
- Comment: Consistent growth in serum tubes requires pretreatment of butyl rubber stoppers by boiling in 1% sodium sulfide solution (1 hr) followed by boiling in distilled H2O (1 hr, twice).
Solutions
Solution A
Dose: 10 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- atribacterium_medium (CultureMech:002438)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.247158Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1272 |
| 2026-03-09T08:04:49.306746+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:13.313223Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002438 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:28.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.415866+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ATRIBACTERIUM MEDIUM" to "atribacterium_medium" for consistent matching |
| 2026-05-16T09:03:25.154251+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:50.047726+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.635583+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:24.427448+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 1e9c1fd952b3ccaf40a2203845061d340028e53ec9020fe591570a3f3dd8c1e3 |