atribacterium_medium

CultureMech:002438 bacterial COMPLEX LIQUID
Original name: ATRIBACTERIUM MEDIUM

Overview

External ontology
mediadive.medium:J1272 (source link unavailable) — ATRIBACTERIUM MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1272

Composition

flowchart LR
    CultureMech_002438["atribacterium_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002438 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_KCl["KCl"]
    CultureMech_002438 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_Na2SO4["Na2SO4"]
    CultureMech_002438 --> ing2_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing2_Na2SO4 -.-> CHEBI_32149
    ing3_NH4Cl["NH4Cl"]
    CultureMech_002438 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_NaH2PO4["NaH2PO4"]
    CultureMech_002438 --> ing4_NaH2PO4
    CHEBI_37585["CHEBI:37585"]
    ing4_NaH2PO4 -.-> CHEBI_37585
    ing5_Xylitol["Xylitol"]
    CultureMech_002438 --> ing5_Xylitol
    CHEBI_17151["CHEBI:17151"]
    ing5_Xylitol -.-> CHEBI_17151
    ing6_Fucose["Fucose"]
    CultureMech_002438 --> ing6_Fucose
    CHEBI_33984["CHEBI:33984"]
    ing6_Fucose -.-> CHEBI_33984
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_002438 --> ing7_Yeast_extract
    ing8_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002438 --> ing8_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing8_Na2S_x_9_H2O -.-> CHEBI_76209
    ing9_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002438 --> ing9_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing9_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002438 --> ing10_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing11_EDTA["EDTA"]
    CultureMech_002438 --> ing11_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing11_EDTA -.-> CHEBI_4735
    ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002438 --> ing12_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002438 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002438 --> ing14_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing14_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002438 --> ing15_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002438 --> ing16_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002438 --> ing17_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing18_H3BO3["H3BO3"]
    CultureMech_002438 --> ing18_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing18_H3BO3 -.-> CHEBI_33118
    ing19_Biotin["Biotin"]
    CultureMech_002438 --> ing19_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing19_Biotin -.-> CHEBI_15956
    ing20_Folic_acid["Folic acid"]
    CultureMech_002438 --> ing20_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing20_Folic_acid -.-> CHEBI_27470
    ing21_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002438 --> ing21_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing21_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing22_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002438 --> ing22_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing22_Thiamine_HCl -.-> CHEBI_49105
    ing23_Riboflavin["Riboflavin"]
    CultureMech_002438 --> ing23_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing23_Riboflavin -.-> CHEBI_17015
    ing24_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002438 --> ing24_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing24_Nicotinic_acid -.-> CHEBI_15940
    ing25_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002438 --> ing25_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing25_Calcium_pantothenate -.-> CHEBI_31345
    ing26_Vitamin_B12["Vitamin B12"]
    CultureMech_002438 --> ing26_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing26_Vitamin_B12 -.-> CHEBI_176843
    ing27_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002438 --> ing27_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing27_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing28_Lipoic_acid["Lipoic acid"]
    CultureMech_002438 --> ing28_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing28_Lipoic_acid -.-> CHEBI_16494

Ingredients (29)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
2.82486 G_PER_L CHEBI:26710
sodium chloride
2 KCl
Cl.K 74.551 g/mol
0.141243 G_PER_L CHEBI:32588
potassium chloride
3 Na2SO4
2Na.O4S 142.043 g/mol
0.282486 G_PER_L CHEBI:32149
sodium sulfate
4 NH4Cl
Cl.H4N 53.492 g/mol
0.100753 G_PER_L CHEBI:31206
ammonium chloride
5 NaH2PO4
H2O4P.Na 119.976 g/mol
1.29944 G_PER_L CHEBI:37585
sodium dihydrogenphosphate
6 Xylitol
C5H12O5 152.146 g/mol
10 G_PER_L CHEBI:17151
xylitol
7 Fucose 10 G_PER_L CHEBI:33984
fucose
8 Yeast extract 10 G_PER_L
9 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
2 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
10 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.115819 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
11 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0141243 G_PER_L CHEBI:86158
calcium chloride dihydrate
12 EDTA
C10H16N2O8 292.244 g/mol
3.7 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
13 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
1.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
14 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.0984 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
15 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.0288 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
16 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.0238 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
17 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.0172 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
18 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.0242 G_PER_L CHEBI:75213
sodium molybdate dihydrate
19 H3BO3
H3BO3 61.833 g/mol
0.031 G_PER_L CHEBI:33118
boric acid
20 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
21 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
22 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
23 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
24 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
25 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
26 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
27 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
28 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
29 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Mix components thoroughly and adjust pH to 7.0 with NaOH. Distribute the medium into culture vessels (e.g., 10.0 ml in 25 ml serum bottles/Balch tubes) under a stream of N2, seal with butyl rubber stoppers and autoclave. After cooling, aseptically add per liter the following solutions (autoclaved or *filter-sterilized and stored anaerobically):
  2. Solution A:
  3. Adjust pH to 6.0 with KOH.
  4. Comment: Consistent growth in serum tubes requires pretreatment of butyl rubber stoppers by boiling in 1% sodium sulfide solution (1 hr) followed by boiling in distilled H2O (1 hr, twice).

Solutions

Solution A

Dose: 10 G_PER_L

See mediadive_5342_Solution_A.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.247158Z mediadive-import Imported from MediaDive Source: JCM, ID: J1272
2026-03-09T08:04:49.306746+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:13.313223Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002438
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:28.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:26.415866+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ATRIBACTERIUM MEDIUM" to "atribacterium_medium" for consistent matching
2026-05-16T09:03:25.154251+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:50.047726+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.635583+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.