Overview
- External ontology
- mediadive.medium:J1276 (source link unavailable) — MODIFIED REINFORCED CLOSTRIDIAL MEDIUM AT pH 8.0
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'modified_reinforced_clostridial_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J1217', 'original_category': 'bacterial'}
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1276
Composition
flowchart LR
CultureMech_002442["modified_reinforced_clostridial_medium_at_ph_8_0"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_002442 --> ing0_Yeast_extract
ing1_Tryptone["Tryptone"]
CultureMech_002442 --> ing1_Tryptone
ing2_Beef_extract["Beef extract"]
CultureMech_002442 --> ing2_Beef_extract
ing3_Glucose["Glucose"]
CultureMech_002442 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002442 --> ing4_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing4_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing5_NaCl["NaCl"]
CultureMech_002442 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_Sodium_acetate["Sodium acetate"]
CultureMech_002442 --> ing6_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing6_Sodium_acetate -.-> CHEBI_32954
ing7_Starch["Starch"]
CultureMech_002442 --> ing7_Starch
CHEBI_28017["CHEBI:28017"]
ing7_Starch -.-> CHEBI_28017
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 3 G_PER_L | ||
| 2 | Tryptone | 10 G_PER_L | ||
| 3 | Beef extract | 10 G_PER_L | ||
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
3 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
3 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 8 | Starch | 1 G_PER_L |
CHEBI:28017
starch
|
Preparation
- Use Medium No. 1217 adjusted pH to 8.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- modified_reinforced_clostridial_medium (CultureMech:002386)
- modified_reinforced_clostridial_medium_at_ph_8_0 (CultureMech:002442)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.261477Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1276 |
| 2026-02-03T11:26:53.686644 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:19:13.337779Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002442 |
| 2026-03-17T19:45:26.444052+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED REINFORCED CLOSTRIDIAL MEDIUM AT pH 8.0" to "modified_reinforced_clostridial_medium_at_ph_8_0" for consistent matching |
| 2026-04-04T18:49:19.081478 | copy_referenced_compositions | RESOLVED_REFERENCE | Resolved reference by copying from modified_reinforced_clostridial_medium (CultureMech:002386) |
| 2026-05-16T08:58:10.194429+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(2 keys) -> list(1 entries) |
| 2026-06-05T07:41:37.681994+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:49.715923+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: modified_reinforced_clostridial_medium, modified_reinforced_clostridial_medium_at_ph_8_0 |
| 2026-08-06T03:05:49.715964+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint d0480c3166977c732ef03db630ac15949f480fd415975d577f32d06977e5de53 |