modified_reinforced_clostridial_medium (CultureMech:002386)

Overview

External ontology

mediadive.medium:J1217 (source link unavailable) — MODIFIED REINFORCED CLOSTRIDIAL MEDIUM

Applications

Microbial cultivation

pH

6.2

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1217

Composition

flowchart LR
    CultureMech_002386["modified_reinforced_clostridial_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_002386 --> ing0_Yeast_extract
    ing1_Tryptone["Tryptone"]
    CultureMech_002386 --> ing1_Tryptone
    ing2_Beef_extract["Beef extract"]
    CultureMech_002386 --> ing2_Beef_extract
    ing3_Glucose["Glucose"]
    CultureMech_002386 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002386 --> ing4_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing4_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing5_NaCl["NaCl"]
    CultureMech_002386 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_Sodium_acetate["Sodium acetate"]
    CultureMech_002386 --> ing6_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing6_Sodium_acetate -.-> CHEBI_32954
    ing7_Starch["Starch"]
    CultureMech_002386 --> ing7_Starch
    CHEBI_28017["CHEBI:28017"]
    ing7_Starch -.-> CHEBI_28017

Ingredients (8)

#	Ingredient	Concentration	Ontology
1	Yeast extract	3 G_PER_L
2	Tryptone	10 G_PER_L
3	Beef extract	10 G_PER_L
4	Glucose C6H12O6 180.156 g/mol	5 G_PER_L	CHEBI:17234 glucose
5	L-Cysteine HCl x H2O C3H7NO2S.H2O.HCl 175.637 g/mol	0.5 G_PER_L	CHEBI:91248 L-cysteine hydrochloride hydrate
6	NaCl Cl.Na 58.443 g/mol	3 G_PER_L	CHEBI:26710 sodium chloride
7	Sodium acetate C2H3O2.Na 82.034 g/mol	3 G_PER_L	CHEBI:32954 sodium acetate
8	Starch	1 G_PER_L	CHEBI:28017 starch

Preparation

Mix components thoroughly and adjust pH to 6.0-6.5. For preparation of solid medium, add per liter 20.0 g agar. Bring to a boil (and dissolve agar), cool down and distribute the medium into culture vessels (e.g., 10 ml medium in Balch type tubes) under a stream of N2, seal with butyl rubber stoppers and autoclave.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:19.057593Z	mediadive-import	Imported from MediaDive	Source: JCM, ID: J1217
2026-03-13T06:19:12.911897Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:002386
2026-03-17T06:30:57.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:25.960936+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "MODIFIED REINFORCED CLOSTRIDIAL MEDIUM" to "modified_reinforced_clostridial_medium" for consistent matching
2026-06-05T07:41:37.675464+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-10-07T15:40:13.763859+00:00	codex	CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE	Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.