Overview
- External ontology
- mediadive.medium:J1296 (source link unavailable) — Hylemonella gracilis medium
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1296
Composition
flowchart LR
CultureMech_002458["hylemonella_gracilis_medium"]
ing0_Bacto_peptone["Bacto peptone"]
CultureMech_002458 --> ing0_Bacto_peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_002458 --> ing1_Yeast_extract
ing2_Tween_80["Tween 80"]
CultureMech_002458 --> ing2_Tween_80
CHEBI_53426["CHEBI:53426"]
ing2_Tween_80 -.-> CHEBI_53426
ing3_KH2PO4["KH2PO4"]
CultureMech_002458 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_Agar["Agar"]
CultureMech_002458 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
ing5_Tap_water["Tap water"]
CultureMech_002458 --> ing5_Tap_water
CHEBI_15377["CHEBI:15377"]
ing5_Tap_water -.-> CHEBI_15377
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto peptone | 5 G_PER_L | ||
| 2 | Yeast extract | 0.5 G_PER_L | ||
| 3 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
0.02 G_PER_L |
CHEBI:53426
polysorbate 80
|
|
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 5 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 6 |
Tap water
H2O 18.015 g/mol
|
1000 G_PER_L |
CHEBI:15377
water
|
Preparation
- Adjust pH to 7.2.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- hylemonella_gracilis_medium (CultureMech:002458)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.346819Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1296 |
| 2026-03-13T06:19:13.489536Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002458 |
| 2026-03-17T06:30:10.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.611990+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Hylemonella gracilis medium" to "hylemonella_gracilis_medium" for consistent matching |
| 2026-06-05T07:41:09.166734+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.976595+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:38.253299+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 4b68fc6ce67f57d2d452f47cca6742141fe34d54fa5f42c7343fc06c832b7a67 |