hylemonella_gracilis_medium

CultureMech:002458 bacterial COMPLEX SOLID_AGAR
Original name: Hylemonella gracilis medium

Overview

External ontology
mediadive.medium:J1296 (source link unavailable) — Hylemonella gracilis medium
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1296

Composition

flowchart LR
    CultureMech_002458["hylemonella_gracilis_medium"]
    ing0_Bacto_peptone["Bacto peptone"]
    CultureMech_002458 --> ing0_Bacto_peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002458 --> ing1_Yeast_extract
    ing2_Tween_80["Tween 80"]
    CultureMech_002458 --> ing2_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing2_Tween_80 -.-> CHEBI_53426
    ing3_KH2PO4["KH2PO4"]
    CultureMech_002458 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_Agar["Agar"]
    CultureMech_002458 --> ing4_Agar
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar -.-> CHEBI_2509
    ing5_Tap_water["Tap water"]
    CultureMech_002458 --> ing5_Tap_water
    CHEBI_15377["CHEBI:15377"]
    ing5_Tap_water -.-> CHEBI_15377

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Bacto peptone 5 G_PER_L
2 Yeast extract 0.5 G_PER_L
3 Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
0.02 G_PER_L CHEBI:53426
polysorbate 80
4 KH2PO4
H2O4P.K 136.084 g/mol
0.1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
5 Agar 15 G_PER_L CHEBI:2509
agar
6 Tap water
H2O 18.015 g/mol
1000 G_PER_L CHEBI:15377
water

Preparation

  1. Adjust pH to 7.2.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.346819Z mediadive-import Imported from MediaDive Source: JCM, ID: J1296
2026-03-13T06:19:13.489536Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002458
2026-03-17T06:30:10.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:26.611990+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Hylemonella gracilis medium" to "hylemonella_gracilis_medium" for consistent matching
2026-06-05T07:41:09.166734+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.976595+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T15:40:13.763859+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.