Overview
- External ontology
- mediadive.medium:J1305 (source link unavailable) — HELICOBACTER MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1305
Composition
flowchart LR
CultureMech_002469["helicobacter_medium"]
ing0_Brucella_Broth["Brucella Broth"]
CultureMech_002469 --> ing0_Brucella_Broth
ing1_Fetal_bovine_serum["Fetal bovine serum"]
CultureMech_002469 --> ing1_Fetal_bovine_serum
ing2_Vitox["Vitox"]
CultureMech_002469 --> ing2_Vitox
ing3_Skirrow_supplement["Skirrow supplement"]
CultureMech_002469 --> ing3_Skirrow_supplement
ing4_Amphotericin_B["Amphotericin B"]
CultureMech_002469 --> ing4_Amphotericin_B
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Brucella Broth | 27.451 G_PER_L | ||
| 2 | Fetal bovine serum | 20 G_PER_L | ||
| 3 | Vitox | 2 G_PER_L | ||
| 4 | Skirrow supplement | 0.4 G_PER_L | ||
| 5 | Amphotericin B | 100 G_PER_L |
mediadive.compound:2063 (source link unavailable)
Amphotericin B
|
Preparation
- Autoclave. After cooling to 50C and add aseptically 135 �l of conc. HCl (final conc. 0.05%). Then add aseptically the following solutions.
- For stable culture, incubate the microorganisms in 5 ml of above-mentioned broth in a polystyrene culture flask (25 cmz2 flask, canted neck, vent cap, AS ONE: Cat. No. 2-8589-01) at 37C under a 5% O2 and 12% CO2 atmosphere (with shaking at low speed; 50 rpm).
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J1305_HELICOBACTER_MEDIUM (CultureMech:002469)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.380366Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1305 |
| 2026-03-13T06:19:13.575888Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002469 |
| 2026-03-17T06:30:10.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.710088+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HELICOBACTER MEDIUM" to "helicobacter_medium" for consistent matching |
| 2026-06-05T07:40:16.687049+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:04:35.382674+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 9cb4659882926c2a152cadb4661884a16fa25496617e9ca5f0aea2203dc31a6d |