Overview
- External ontology
- mediadive.medium:J1305 (source link unavailable) — HELICOBACTER MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1305
Composition
flowchart LR
CultureMech_002469["helicobacter_medium"]
ing0_Brucella_broth__BD_BBL_["Brucella broth (BD-BBL)"]
CultureMech_002469 --> ing0_Brucella_broth__BD_BBL_
ing1_Distilled_water["Distilled water"]
CultureMech_002469 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_Conc__HCl["Conc. HCl"]
CultureMech_002469 --> ing2_Conc__HCl
CHEBI_17883["CHEBI:17883"]
ing2_Conc__HCl -.-> CHEBI_17883
ing3_Fetal_bovine_serum__Biowest__heat_inactivated_["Fetal bovine serum (Biowest; heat inactivated)"]
CultureMech_002469 --> ing3_Fetal_bovine_serum__Biowest__heat_inactivated_
ing4_Vitox__Oxoid_["Vitox (Oxoid)"]
CultureMech_002469 --> ing4_Vitox__Oxoid_
ing5_Skirrow_supplement__Oxoid_["Skirrow supplement (Oxoid)"]
CultureMech_002469 --> ing5_Skirrow_supplement__Oxoid_
ing6_Amphotericin_B__5_mg_ml_DMSO_["Amphotericin B (5 mg/ml DMSO)"]
CultureMech_002469 --> ing6_Amphotericin_B__5_mg_ml_DMSO_
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Brucella broth (BD-BBL) | 28 G_PER_L | JCM Medium 1305 lists 2.8 g Brucella broth (BD-BBL) in an 80 ml distilled-water base; this row is normalized 10x to one liter and the BD-BBL product is not reducible to one ChEBI molecule. | |
| 2 |
Distilled water
H2O 18.015 g/mol
|
800 ML_PER_L |
CHEBI:15377
water
|
JCM Medium 1305 lists 80.0 ml distilled water in the source batch; this row is normalized 10x to one liter. |
| 3 |
Conc. HCl
HCl 36.461 g/mol
|
1.35 ML_PER_L |
CHEBI:17883
hydrogen chloride
|
JCM Medium 1305 adds 135 microliter concentrated HCl after autoclaving; this row is normalized 10x to one liter. |
| 4 | Fetal bovine serum (Biowest; heat inactivated) | 200 ML_PER_L | JCM Medium 1305 aseptically adds 20.0 ml heat-inactivated fetal bovine serum from Biowest; this row is normalized 10x to one liter. | |
| 5 | Vitox (Oxoid) | 20 ML_PER_L | JCM Medium 1305 aseptically adds 2.0 ml Vitox prepared from one Oxoid vial in 10 ml rehydration fluid; this row is normalized 10x to one liter. | |
| 6 | Skirrow supplement (Oxoid) | 4 ML_PER_L | JCM Medium 1305 aseptically adds 0.4 ml Skirrow supplement prepared from one Oxoid vial in 2 ml sterile purified water; this row is normalized 10x to one liter. | |
| 7 | Amphotericin B (5 mg/ml DMSO) | 1 ML_PER_L | JCM Medium 1305 aseptically adds 100 microliter of the 5 mg/ml amphotericin B in DMSO stock; this row is normalized 10x to one liter and represents a stock solution, not neat amphotericin B. |
Preparation
- Autoclave Brucella broth in distilled water and cool to 50 C.
- Aseptically add concentrated HCl, heat-inactivated fetal bovine serum, Vitox, Skirrow supplement, and amphotericin B in DMSO at the JCM Medium 1305 proportions.
- For stable culture, incubate 5 ml broth in a 25 cm2 polystyrene culture flask at 37 C under 5% O2 and 12% CO2 with shaking at 50 rpm.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.380366Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1305 |
| 2026-03-13T06:19:13.575888Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002469 |
| 2026-03-17T06:30:10.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.710088+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HELICOBACTER MEDIUM" to "helicobacter_medium" for consistent matching |
| 2026-06-05T07:40:16.687049+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-11T00:00:00-07:00 | repair_jcm_1305_helicobacter_score15.py | RESOLVED_JCM_1305_HELICOBACTER_SCORE15 | JCM Medium 1305 lists an 80 ml Brucella broth base acidified with 135 microliter concentrated HCl after autoclaving, then aseptic addition of 20 ml heat-inactivated fetal bovine serum, 2 ml Vitox, 0.4 ml Skirrow supplement, and 100 microliter amphotericin B stock; amounts are normalized 10x from the source batch. |