mineral_medium_for_hydrogenophilus_ss56

CultureMech:002471 bacterial COMPLEX LIQUID
Original name: MINERAL MEDIUM FOR HYDROGENOPHILUS SS56

Overview

External ontology
mediadive.medium:J1308 (source link unavailable) — MINERAL MEDIUM FOR HYDROGENOPHILUS SS56
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'medium_for_hydrogenophilus', 'source': 'MediaDive', 'source_id': 'mediadive.medium:1733', 'original_category': 'bacterial'}
pH
6.8
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1308

Composition

flowchart LR
    CultureMech_002471["mineral_medium_for_hydrogenophilus_ss56"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002471 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_Na2HPO4_x_6_H2O["Na2HPO4 x 6 H2O"]
    CultureMech_002471 --> ing1_Na2HPO4_x_6_H2O
    CHEBI_34683["CHEBI:34683"]
    ing1_Na2HPO4_x_6_H2O -.-> CHEBI_34683
    ing2_NH4Cl["NH4Cl"]
    CultureMech_002471 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_NaHCO3["NaHCO3"]
    CultureMech_002471 --> ing3_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing3_NaHCO3 -.-> CHEBI_32139
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002471 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002471 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_Fe_NH4_citrate["Fe(NH4)citrate"]
    CultureMech_002471 --> ing6_Fe_NH4_citrate
    CHEBI_31604["CHEBI:31604"]
    ing6_Fe_NH4_citrate -.-> CHEBI_31604
    ing7_Sodium_lactate["Sodium lactate"]
    CultureMech_002471 --> ing7_Sodium_lactate
    CHEBI_75228["CHEBI:75228"]
    ing7_Sodium_lactate -.-> CHEBI_75228
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_002471 --> ing8_Yeast_extract
    ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002471 --> ing9_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002471 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_H3BO3["H3BO3"]
    CultureMech_002471 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002471 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002471 --> ing13_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002471 --> ing14_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002471 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
2.59887 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 Na2HPO4 x 6 H2O
HO4P.2Na 141.958 g/mol
3.27684 G_PER_L CHEBI:34683
disodium hydrogenphosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
1.12994 G_PER_L CHEBI:31206
ammonium chloride
4 NaHCO3
CHO3.Na 84.006 g/mol
0.564972 G_PER_L CHEBI:32139
sodium hydrogencarbonate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.112994 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.01 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
7 Fe(NH4)citrate 0.0004 G_PER_L CHEBI:31604
ferric ammonium citrate
8 Sodium lactate
C3H5O3.Na 112.06 g/mol
0.04 G_PER_L CHEBI:75228
sodium lactate
9 Yeast extract 0.002 G_PER_L
10 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
11 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
12 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
15 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
16 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix components thoroughly, adjust pH to 6.8 and autoclave. After cooling, add the following solutions (autoclaved):
  2. Aseptically distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) and seal with butyl rubber stoppers. Replace the gas phase with N2 containing 5-10% O2 (by volume).
  3. Adjust pH to 3.6.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:19.387256Z mediadive-import Imported from MediaDive Source: JCM, ID: J1308
2026-03-13T06:19:13.596503Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002471
2026-03-17T06:30:11.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:26.733118+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MINERAL MEDIUM FOR HYDROGENOPHILUS SS56" to "mineral_medium_for_hydrogenophilus_ss56" for consistent matching
2026-06-05T07:41:34.156447+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:07.884726+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:43.135152+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: medium_for_hydrogenophilus, mineral_medium_for_hydrogenophilus_ss56
2026-08-06T03:05:43.135201+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint 05b305ae3208a38027cce9c35d51dc9cf7de07d832d4740819d81cfb8d37552d