medium_for_hydrogenophilus

CultureMech:001210 bacterial COMPLEX LIQUID
Original name: MEDIUM FOR HYDROGENOPHILUS

Overview

External ontology
mediadive.medium:1733 (source link unavailable) — MEDIUM FOR HYDROGENOPHILUS
Applications
  • Microbial cultivation
pH
6.8
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1733.pdf

Composition

flowchart LR
    CultureMech_001210["medium_for_hydrogenophilus"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_001210 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_Na2HPO4_x_6_H2O["Na2HPO4 x 6 H2O"]
    CultureMech_001210 --> ing1_Na2HPO4_x_6_H2O
    CHEBI_34683["CHEBI:34683"]
    ing1_Na2HPO4_x_6_H2O -.-> CHEBI_34683
    ing2_NH4Cl["NH4Cl"]
    CultureMech_001210 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_NaHCO3["NaHCO3"]
    CultureMech_001210 --> ing3_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing3_NaHCO3 -.-> CHEBI_32139
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001210 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001210 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_Fe_NH4_citrate["Fe(NH4)citrate"]
    CultureMech_001210 --> ing6_Fe_NH4_citrate
    CHEBI_31604["CHEBI:31604"]
    ing6_Fe_NH4_citrate -.-> CHEBI_31604
    ing7_Sodium_lactate["Sodium lactate"]
    CultureMech_001210 --> ing7_Sodium_lactate
    CHEBI_75228["CHEBI:75228"]
    ing7_Sodium_lactate -.-> CHEBI_75228
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_001210 --> ing8_Yeast_extract
    ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001210 --> ing9_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001210 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_H3BO3["H3BO3"]
    CultureMech_001210 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001210 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001210 --> ing13_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001210 --> ing14_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001210 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
2.59887 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 Na2HPO4 x 6 H2O
HO4P.2Na 141.958 g/mol
3.27684 G_PER_L CHEBI:34683
disodium hydrogenphosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
1.12994 G_PER_L CHEBI:31206
ammonium chloride
4 NaHCO3
CHO3.Na 84.006 g/mol
0.564972 G_PER_L CHEBI:32139
sodium hydrogencarbonate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.112994 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.01 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
7 Fe(NH4)citrate 0.0004 G_PER_L CHEBI:31604
ferric ammonium citrate
8 Sodium lactate
C3H5O3.Na 112.06 g/mol
0.04 G_PER_L CHEBI:75228
sodium lactate
9 Yeast extract 0.002 G_PER_L
10 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
11 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
12 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
15 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
16 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix components thoroughly, adjust pH to 6.8. Distribute the medium into culture vessels (e.g., 17.0 ml in 120 ml serum bottles), seal with butyl rubber stoppers and autoclave.
  2. After cooling, add the following autoclaved solutions (for final 20 ml):
  3. Pressurize to 100 kPa with H2-CO2 (4:1, v/v).
  4. Add 10% O2 to headspace

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.384702Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1733
2026-03-13T06:19:02.273177Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001210
2026-03-17T06:30:32.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.088130+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MEDIUM FOR HYDROGENOPHILUS" to "medium_for_hydrogenophilus" for consistent matching
2026-06-05T07:41:29.109906+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.063532+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.