bl_agar_glucose_blood_liver_agar

CultureMech:002499 bacterial COMPLEX SOLID_AGAR
Original name: BL AGAR (GLUCOSE BLOOD LIVER AGAR)

Overview

External ontology
mediadive.medium:J13 (source link unavailable) — BL AGAR (GLUCOSE BLOOD LIVER AGAR)
Applications
  • Microbial cultivation
pH
7.2
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=13

Composition

flowchart LR
    CultureMech_002499["bl_agar_glucose_blood_liver_agar"]
    ing0_Lab_Lemco_beef_extract["Lab-Lemco beef extract"]
    CultureMech_002499 --> ing0_Lab_Lemco_beef_extract
    ing1_Proteose_peptone_no__3["Proteose peptone no. 3"]
    CultureMech_002499 --> ing1_Proteose_peptone_no__3
    ing2_Trypticase_peptone["Trypticase peptone"]
    CultureMech_002499 --> ing2_Trypticase_peptone
    ing3_Phytone_peptone["Phytone peptone"]
    CultureMech_002499 --> ing3_Phytone_peptone
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_002499 --> ing4_Yeast_extract
    ing5_Liver_extract["Liver extract"]
    CultureMech_002499 --> ing5_Liver_extract
    ing6_Glucose["Glucose"]
    CultureMech_002499 --> ing6_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing6_Glucose -.-> CHEBI_17234
    ing7_Starch["Starch"]
    CultureMech_002499 --> ing7_Starch
    CHEBI_28017["CHEBI:28017"]
    ing7_Starch -.-> CHEBI_28017
    ing8_Tween_80["Tween 80"]
    CultureMech_002499 --> ing8_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing8_Tween_80 -.-> CHEBI_53426
    ing9_Agar["Agar"]
    CultureMech_002499 --> ing9_Agar
    CHEBI_2509["CHEBI:2509"]
    ing9_Agar -.-> CHEBI_2509
    ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002499 --> ing10_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing11_Horse_blood["Horse blood"]
    CultureMech_002499 --> ing11_Horse_blood
    ing12_K2HPO4["K2HPO4"]
    CultureMech_002499 --> ing12_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing12_K2HPO4 -.-> CHEBI_131527
    ing13_KH2PO4["KH2PO4"]
    CultureMech_002499 --> ing13_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing13_KH2PO4 -.-> CHEBI_63036
    ing14_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002499 --> ing14_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing14_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing15_NaCl["NaCl"]
    CultureMech_002499 --> ing15_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing15_NaCl -.-> CHEBI_26710
    ing16_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002499 --> ing16_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing16_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing17_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002499 --> ing17_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing17_MnSO4_x_n_H2O -.-> CHEBI_86360

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 Lab-Lemco beef extract 2.85714 G_PER_L
2 Proteose peptone no. 3 9.52381 G_PER_L
3 Trypticase peptone 4.7619 G_PER_L
4 Phytone peptone 2.85714 G_PER_L
5 Yeast extract 4.7619 G_PER_L
6 Liver extract 150 G_PER_L
7 Glucose
C6H12O6 180.156 g/mol
9.52381 G_PER_L CHEBI:17234
glucose
8 Starch 0.47619 G_PER_L CHEBI:28017
starch
9 Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
0.952381 G_PER_L CHEBI:53426
polysorbate 80
10 Agar 14.2857 G_PER_L CHEBI:2509
agar
11 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
10 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
12 Horse blood 50 G_PER_L
13 K2HPO4
HO4P.2K 174.174 g/mol
100 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
14 KH2PO4
H2O4P.K 136.084 g/mol
100 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
15 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
40 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
16 NaCl
Cl.Na 58.443 g/mol
2 G_PER_L CHEBI:26710
sodium chloride
17 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
18 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
2 G_PER_L CHEBI:86360
manganese(II) sulfate

Preparation

  1. Adjust pH to 7.2.
  2. To prepare liver extract, put 10 g liver powder in 170 ml water, keep at 50 to 60C for 1 hr, boil for 5 min, adjust pH to 7.2 and filter.
  3. Mix ingredients except horse blood and adjust pH to 7.2. After autoclaving, cool to 50C, and aseptically add 50.0 ml of horse blood.
  4. Comment: Commercial BL agar (Nissui) is also available.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:15.579443Z mediadive-import Imported from MediaDive Source: JCM, ID: J13
2026-03-13T06:19:13.836124Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002499
2026-03-17T06:30:10.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:27.002612+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BL AGAR (GLUCOSE BLOOD LIVER AGAR)" to "bl_agar_glucose_blood_liver_agar" for consistent matching
2026-06-05T07:40:51.015538+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.914913+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:25.444636+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 882e46120b6617c5069981dbe079ad95fd671abb1098171d84ab8cd6f8eaf8ad