bl_agar_glucose_blood_liver_agar

CultureMech:002499 bacterial COMPLEX SOLID_AGAR
Original name: BL AGAR (GLUCOSE BLOOD LIVER AGAR)

Overview

External ontology
mediadive.medium:J13 (source link unavailable) — BL AGAR (GLUCOSE BLOOD LIVER AGAR)
Applications
  • Microbial cultivation
pH
7.2
Notes
JCM Medium 13 BL Agar lists Lab-Lemco powder, three peptone products, yeast extract, liver extract, glucose, soluble starch, Solution A, Solution B, Tween 80, agar, 5% L-Cysteine HCl x H2O solution, horse blood, and distilled water; the formula is adjusted to pH 7.2 and horse blood is added aseptically after autoclaving and cooling.

Composition

flowchart LR
    CultureMech_002499["bl_agar_glucose_blood_liver_agar"]
    ing0_Lab_Lemco_powder__Oxoid_["Lab-Lemco powder (Oxoid)"]
    CultureMech_002499 --> ing0_Lab_Lemco_powder__Oxoid_
    ing1_Proteose_peptone_No__3__BD_Difco_["Proteose peptone No. 3 (BD-Difco)"]
    CultureMech_002499 --> ing1_Proteose_peptone_No__3__BD_Difco_
    ing2_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
    CultureMech_002499 --> ing2_Trypticase_peptone__BD_BBL_
    ing3_Phytone_peptone__BD_BBL_["Phytone peptone (BD-BBL)"]
    CultureMech_002499 --> ing3_Phytone_peptone__BD_BBL_
    ing4_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_002499 --> ing4_Yeast_extract__BD_Difco_
    ing5_Glucose["Glucose"]
    CultureMech_002499 --> ing5_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing5_Glucose -.-> CHEBI_17234
    ing6_Soluble_starch["Soluble starch"]
    CultureMech_002499 --> ing6_Soluble_starch
    CHEBI_28017["CHEBI:28017"]
    ing6_Soluble_starch -.-> CHEBI_28017
    ing7_Tween_80["Tween 80"]
    CultureMech_002499 --> ing7_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing7_Tween_80 -.-> CHEBI_53426
    ing8_Bacto_agar__BD_Difco_["Bacto agar (BD-Difco)"]
    CultureMech_002499 --> ing8_Bacto_agar__BD_Difco_
    CHEBI_2509["CHEBI:2509"]
    ing8_Bacto_agar__BD_Difco_ -.-> CHEBI_2509
    ing9_Horse_blood["Horse blood"]
    CultureMech_002499 --> ing9_Horse_blood
    ing10_Distilled_water["Distilled water"]
    CultureMech_002499 --> ing10_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing10_Distilled_water -.-> CHEBI_15377

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Lab-Lemco powder (Oxoid) 3.0 G_PER_L FOODON:03302088
beef extract
 MediaDive J13 / JCM Medium 13 lists 3.0 g/L Lab-Lemco powder (Oxoid), a source-disclosed beef-extract product.
2 Proteose peptone No. 3 (BD-Difco) 10.0 G_PER_L MICRO:0000180 (source link unavailable)
proteose peptone
 MediaDive J13 / JCM Medium 13 lists 10.0 g/L Proteose peptone No. 3 (BD-Difco).
3 Trypticase peptone (BD-BBL) 5.0 G_PER_L MICRO:0000175 (source link unavailable)
Trypticase peptone
 MediaDive J13 / JCM Medium 13 lists 5.0 g/L Trypticase peptone (BD-BBL).
4 Phytone peptone (BD-BBL) 3.0 G_PER_L MICRO:0000178 (source link unavailable)
peptone
 MediaDive J13 / JCM Medium 13 lists 3.0 g/L Phytone peptone (BD-BBL).
5 Yeast extract (BD-Difco) 5.0 G_PER_L FOODON:03315426
yeast extract
 MediaDive J13 / JCM Medium 13 lists 5.0 g/L Yeast extract (BD-Difco).
6 Glucose
C6H12O6 180.156 g/mol
10.0 G_PER_L CHEBI:17234
glucose
 MediaDive J13 / JCM Medium 13 lists 10.0 g/L Glucose.
7 Soluble starch 0.5 G_PER_L CHEBI:28017
starch
 MediaDive J13 / JCM Medium 13 lists 0.5 g/L Soluble starch.
8 Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
1.0 G_PER_L CHEBI:53426
polysorbate 80
 MediaDive J13 / JCM Medium 13 lists 1.0 g/L Tween 80.
9 Bacto agar (BD-Difco) 15.0 G_PER_L CHEBI:2509
agar
 MediaDive J13 / JCM Medium 13 lists 15.0 g/L Bacto agar (BD-Difco).
10 Horse blood 50.0 ML_PER_L UBERON:0000178
blood
 MediaDive J13 / JCM Medium 13 lists 50.0 ml/L Horse blood.
11 Distilled water
H2O 18.015 g/mol
825.0 ML_PER_L CHEBI:15377
water
 MediaDive J13 / JCM Medium 13 lists 825.0 ml/L Distilled water.

Preparation

  1. Prepare Liver extract by putting 10.0 g liver powder in 170.0 ml water, holding at 50-60 C for 1 hr, boiling for 5 min, adjusting pH to 7.2, and filtering.
  2. Combine the base ingredients, 150.0 ml/L Liver extract, 10.0 ml/L Solution A, 5.0 ml/L Solution B, 10.0 ml/L 5% L-Cysteine HCl x H2O solution, and 825.0 ml/L distilled water; omit horse blood before autoclaving.
  3. Adjust pH to 7.2 before autoclaving.
  4. Autoclave at 121 C for 15 min.
  5. Cool to 50 C and aseptically add 50.0 ml/L horse blood.

Solutions

Liver extract

Dose: 150.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Liver powder 58.8235 G_PER_L JCM Medium 13 prepares Liver extract from 10.0 g liver powder in 170.0 ml water before heating, pH adjustment, and filtration.
Distilled water 1000.0 ML_PER_L JCM Medium 13 prepares Liver extract by suspending 10.0 g liver powder in 170.0 ml water.
MediaDive J13 / JCM Medium 13 adds 150.0 ml/L Liver extract prepared as below.

Solution A

Dose: 10.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
K2HPO4 100.0 G_PER_L MediaDive J13 / JCM Medium 13 lists 100.0 g/L K2HPO4.
KH2PO4 100.0 G_PER_L MediaDive J13 / JCM Medium 13 lists 100.0 g/L KH2PO4.
Distilled water 1000.0 ML_PER_L MediaDive J13 / JCM Medium 13 lists 1000.0 ml/L Distilled water.
MediaDive J13 / JCM Medium 13 adds 10.0 ml/L Solution A.

Solution B

Dose: 5.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
MgSO4 x 7 H2O 40.0 G_PER_L MediaDive J13 / JCM Medium 13 lists 40.0 g/L MgSO4 x 7 H2O.
NaCl 2.0 G_PER_L MediaDive J13 / JCM Medium 13 lists 2.0 g/L NaCl.
FeSO4 x 7 H2O 2.0 G_PER_L MediaDive J13 / JCM Medium 13 lists 2.0 g/L FeSO4 x 7 H2O.
MnSO4 x n H2O 2.0 G_PER_L JCM Medium 13 lists 0.2 g MnSO4 x H2O of unspecified hydrate number per 100.0 ml Solution B.
Distilled water 1000.0 ML_PER_L MediaDive J13 / JCM Medium 13 lists 1000.0 ml/L Distilled water.
MediaDive J13 / JCM Medium 13 adds 5.0 ml/L Solution B.

5% L-Cysteine HCl x H2O solution

Dose: 10.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
L-Cysteine HCl x H2O 5.0 PERCENT_W_V JCM Medium 13 specifies the added L-Cysteine HCl x H2O solution as 5% w/v.
MediaDive J13 / JCM Medium 13 adds 10.0 ml/L 5% L-Cysteine HCl x H2O solution.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.579443Z mediadive-import Imported from MediaDive Source: JCM, ID: J13
2026-03-13T06:19:13.836124Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002499
2026-03-17T06:30:10.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:27.002612+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BL AGAR (GLUCOSE BLOOD LIVER AGAR)" to "bl_agar_glucose_blood_liver_agar" for consistent matching
2026-06-05T07:40:51.015538+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.914913+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-11T00:00:00-07:00 repair_togo_m6_score15.py RESOLVED_JCM_13_BL_AGAR Restored JCM Medium 13 direct ingredients, moved Liver extract, Solution A, Solution B, and the 5% L-Cysteine HCl x H2O solution from flattened top-level rows into solution entries, grounded source-disclosed components, and linked TOGO M6 plus the two BL With 0.5% Maltose children.