Overview
- External ontology
- mediadive.medium:J151 (source link unavailable) — PYROCOCCUS MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'jcm_medium_no_261', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J261', 'original_category': 'bacterial'}
- pH
- 6.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=151
Composition
flowchart LR
CultureMech_002510["pyrococcus_medium"]
ing0_Citric_acid["Citric acid"]
CultureMech_002510 --> ing0_Citric_acid
CHEBI_30769["CHEBI:30769"]
ing0_Citric_acid -.-> CHEBI_30769
ing1_Yeast_extract["Yeast extract"]
CultureMech_002510 --> ing1_Yeast_extract
ing2_Bacto_peptone["Bacto peptone"]
CultureMech_002510 --> ing2_Bacto_peptone
ing3_Sulfur["Sulfur"]
CultureMech_002510 --> ing3_Sulfur
CHEBI_26833["CHEBI:26833"]
ing3_Sulfur -.-> CHEBI_26833
ing4_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002510 --> ing4_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing4_Na2S_x_9_H2O -.-> CHEBI_76209
ing5_Resazurin["Resazurin"]
CultureMech_002510 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_NaCl["NaCl"]
CultureMech_002510 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002510 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002510 --> ing8_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing8_MgCl2_x_6_H2O -.-> CHEBI_86345
ing9_KCl["KCl"]
CultureMech_002510 --> ing9_KCl
CHEBI_32588["CHEBI:32588"]
ing9_KCl -.-> CHEBI_32588
ing10_NaBr["NaBr"]
CultureMech_002510 --> ing10_NaBr
CHEBI_63004["CHEBI:63004"]
ing10_NaBr -.-> CHEBI_63004
ing11_H3BO3["H3BO3"]
CultureMech_002510 --> ing11_H3BO3
CHEBI_33118["CHEBI:33118"]
ing11_H3BO3 -.-> CHEBI_33118
ing12_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_002510 --> ing12_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing12_SrCl2_x_6_H2O -.-> CHEBI_36385
ing13_KI["KI"]
CultureMech_002510 --> ing13_KI
CHEBI_8346["CHEBI:8346"]
ing13_KI -.-> CHEBI_8346
ing14_CaCl2["CaCl2"]
CultureMech_002510 --> ing14_CaCl2
CHEBI_3312["CHEBI:3312"]
ing14_CaCl2 -.-> CHEBI_3312
ing15_KH2PO4["KH2PO4"]
CultureMech_002510 --> ing15_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing15_KH2PO4 -.-> CHEBI_63036
ing16__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
CultureMech_002510 --> ing16__NH4_2Ni_SO4_2_x_6_H2O
CHEBI_86149["CHEBI:86149"]
ing16__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
ing17_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002510 --> ing17_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing17_Nitrilotriacetic_acid -.-> CHEBI_44557
ing18_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002510 --> ing18_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing18_MnSO4_x_n_H2O -.-> CHEBI_86360
ing19_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002510 --> ing19_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing19_FeSO4_x_7_H2O -.-> CHEBI_75836
ing20_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002510 --> ing20_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing20_CoSO4_x_7_H2O -.-> CHEBI_91244
ing21_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002510 --> ing21_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing21_CaCl2_x_2_H2O -.-> CHEBI_86158
ing22_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002510 --> ing22_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing22_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing23_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002510 --> ing23_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing23_CuSO4_x_5_H2O -.-> CHEBI_31440
ing24_AlK_SO4_2["AlK(SO4)2"]
CultureMech_002510 --> ing24_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing24_AlK_SO4_2 -.-> CHEBI_86463
ing25_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002510 --> ing25_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing25_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (26)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Citric acid
C6H8O7 192.123 g/mol
|
0.00495049 G_PER_L |
CHEBI:30769
citric acid
|
|
| 2 | Yeast extract | 0.990099 G_PER_L | ||
| 3 | Bacto peptone | 4.9505 G_PER_L | ||
| 4 |
Sulfur
S 32.067 g/mol
|
29.703 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 5 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.49505 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000990099 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
14.85 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 13.85, 1.0] |
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
6.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 3.5, 3.0] |
| 9 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.75 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 10 |
KCl
Cl.K 74.551 g/mol
|
0.325 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 11 |
NaBr
Br.Na 102.894 g/mol
|
0.05 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 12 |
H3BO3
H3BO3 61.833 g/mol
|
0.025 G_PER_L |
CHEBI:33118
boric acid
|
[Merged 2 duplicates: 0.015, 0.01] |
| 13 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.0075 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 14 |
KI
I.K 166.002 g/mol
|
5e-05 G_PER_L |
CHEBI:8346
potassium iodide
|
|
| 15 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.75 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 16 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 17 |
(NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
|
0.002 G_PER_L |
CHEBI:86149
ammonium nickel sulfate hexahydrate
|
|
| 18 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 19 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 20 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 21 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 22 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 23 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 24 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 25 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 26 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Mix ingredients, except sulfur and Na2S x 9H2O, and adjust pH to 6.5 with H2SO4. Sterilize by filtration under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Neutralize Na2S x 9H2O as a 5% solution, and autoclave under a N2 atmosphere. Aseptically and anaerobically add sterile sulfur to the medium. Prior to inoculation, add the sterile Na2S x 9H2O solution, and readjust pH to 6.5, if necessary.
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- JCM_J151_PYROCOCCUS_MEDIUM (CultureMech:002510)
- jcm_medium_no_261 (CultureMech:002619)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.825983Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J151 |
| 2026-03-13T03:09:39.671970Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:13.895413Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002510 |
| 2026-03-17T06:30:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.067522+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYROCOCCUS MEDIUM" to "pyrococcus_medium" for consistent matching |
| 2026-06-05T07:40:16.712141+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.789062+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:20.628424+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: JCM_J151_PYROCOCCUS_MEDIUM, jcm_medium_no_261 |
| 2026-08-06T03:04:20.628467+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint e417c7c0499106928dffbba40c2700ddd1cfca3417eb43ee4dcb06e96cb88375 |