Overview
- External ontology
- mediadive.medium:J261 (source link unavailable) — JCM MEDIUM No. 261
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=261
Composition
flowchart LR
CultureMech_002619["jcm_medium_no_261"]
ing0_Citric_acid["Citric acid"]
CultureMech_002619 --> ing0_Citric_acid
CHEBI_30769["CHEBI:30769"]
ing0_Citric_acid -.-> CHEBI_30769
ing1_Resazurin["Resazurin"]
CultureMech_002619 --> ing1_Resazurin
CHEBI_8806["CHEBI:8806"]
ing1_Resazurin -.-> CHEBI_8806
ing2_Yeast_extract["Yeast extract"]
CultureMech_002619 --> ing2_Yeast_extract
ing3_Bacto_peptone["Bacto peptone"]
CultureMech_002619 --> ing3_Bacto_peptone
ing4_Na2S2O3_x_5H2O["Na2S2O3 x 5H2O"]
CultureMech_002619 --> ing4_Na2S2O3_x_5H2O
ing5_H2SO4["H2SO4"]
CultureMech_002619 --> ing5_H2SO4
ing6_N2["N2"]
CultureMech_002619 --> ing6_N2
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Citric acid
C6H8O7 192.123 g/mol
|
CHEBI:30769
citric acid
|
Copied from referenced JCM Medium 151 Source batch input: 5 mg. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 2 |
Resazurin
C12H7NO4 229.191 g/mol
|
CHEBI:8806
Resazurin
|
Copied from referenced JCM Medium 151 Source batch input: 1 mg. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 3 | Yeast extract | 2 G_PER_L | Copied from referenced JCM Medium 151 Explicit final variant concentration; replaces the parent's amount, not added on top of it. No inferred final-volume arithmetic. | |
| 4 | Bacto peptone | 2 G_PER_L | Copied from referenced JCM Medium 151 Explicit final variant concentration; replaces the parent's amount, not added on top of it. No inferred final-volume arithmetic. | |
| 5 | Na2S2O3 x 5H2O | 1 G_PER_L | Explicit final variant concentration; replaces the parent's amount, not added on top of it. No inferred final-volume arithmetic. | |
| 6 | H2SO4 | Inherited preparation reagent; no fixed dose specified. Receiving-medium pH is overridden to 7.0. | ||
| 7 | N2 | Inherited preparation reagent; no fixed dose specified. Receiving-medium pH is overridden to 7.0. |
Preparation
- Use Medium No. 151 with 2.0 g/L (final) yeast extract, 2.0 g/L (final) peptone and 1.0 g/L Na2S2O3 x 5H2O, and omit sulfur. Adjust pH to 7.0.
- Apply the base-medium filtration under N2, with sulfur omitted and sodium sulfide prepared separately. The variant overrides receiving-medium pH to 7.0.
- Before inoculation, add the separately prepared sterile sodium sulfide solution. Apply the variant's pH 7.0 override. No sulfur preparation or addition is performed.
Solutions
Salt base solution
Dose: 1 L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaCl | 13.85 G_PER_L | [Merged 2 duplicates: 13.85, 1.0] | Copied from JCM Medium 151 Source stock batch input: 13.85 g. Retain provider-declared stock g_l=13.85 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| MgSO4 x 7 H2O | 3.5 G_PER_L | [Merged 2 duplicates: 3.5, 3.0] | Copied from JCM Medium 151 Source stock batch input: 3.5 g. Retain provider-declared stock g_l=3.5 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| MgCl2 x 6 H2O | 2.75 G_PER_L | Copied from referenced JCM Medium 151 Source stock batch input: 2.75 g. Retain provider-declared stock g_l=2.75 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| KCl | 0.325 G_PER_L | Copied from referenced JCM Medium 151 Source stock batch input: 0.325 g. Retain provider-declared stock g_l=0.325 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| NaBr | 0.05 G_PER_L | Copied from referenced JCM Medium 151 Source stock batch input: 0.05 g. Retain provider-declared stock g_l=0.05 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| H3BO3 | 0.015 G_PER_L | [Merged 2 duplicates: 0.015, 0.01] | Copied from JCM Medium 151 Source stock batch input: 15 mg. Retain provider-declared stock g_l=0.015 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| SrCl2 x 6 H2O | 0.0075 G_PER_L | Copied from referenced JCM Medium 151 Source stock batch input: 7.5 mg. Retain provider-declared stock g_l=0.0075 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| KI | 0.00005 G_PER_L | Copied from referenced JCM Medium 151 Source stock batch input: 0.05 mg. Retain provider-declared stock g_l=5e-05 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| CaCl2 | 0.75 G_PER_L | Copied from referenced JCM Medium 151 Source stock batch input: 0.75 g. Retain provider-declared stock g_l=0.75 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| KH2PO4 | 0.5 G_PER_L | Copied from referenced JCM Medium 151 Source stock batch input: 0.5 g. Retain provider-declared stock g_l=0.5 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| (NH4)2Ni(SO4)2 x 6 H2O | 0.002 G_PER_L | Copied from referenced JCM Medium 151 Source stock batch input: 2 mg. Retain provider-declared stock g_l=0.002 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Distilled water | 1 L | Source water batch input: 1000 ml = 1 L. This is not a measured final volume. Prior duplicate-collapse notes describe the superseded flattened record. |
Source stock addition: 1000 ml = 1 L per receiving batch, not grams per liter or a dose per final liter.
Trace minerals
Dose: 0.01 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Nitrilotriacetic acid | 1.5 G_PER_L | Copied from referenced JCM Medium 151 Source stock batch input: 1.5 g. Retain provider-declared stock g_l=1.5 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| MgSO4 x 7 H2O | 3 G_PER_L | [Merged 2 duplicates: 3.5, 3.0] | Copied from JCM Medium 151 Source stock batch input: 3 g. Retain provider-declared stock g_l=3 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| MnSO4 x n H2O | 0.5 G_PER_L | Copied from referenced JCM Medium 151 Source stock batch input: 0.5 g. Retain provider-declared stock g_l=0.5 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| NaCl | 1 G_PER_L | [Merged 2 duplicates: 13.85, 1.0] | Copied from JCM Medium 151 Source stock batch input: 1 g. Retain provider-declared stock g_l=1 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| FeSO4 x 7 H2O | 0.1 G_PER_L | Copied from referenced JCM Medium 151 Source stock batch input: 0.1 g. Retain provider-declared stock g_l=0.1 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| CoSO4 x 7 H2O | 0.1 G_PER_L | Copied from referenced JCM Medium 151 Source stock batch input: 0.1 g. Retain provider-declared stock g_l=0.1 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| CaCl2 x 2 H2O | 0.1 G_PER_L | Copied from referenced JCM Medium 151 Source stock batch input: 0.1 g. Retain provider-declared stock g_l=0.1 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| ZnSO4 x 7 H2O | 0.1 G_PER_L | Copied from referenced JCM Medium 151 Source stock batch input: 0.1 g. Retain provider-declared stock g_l=0.1 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| CuSO4 x 5 H2O | 0.01 G_PER_L | Copied from referenced JCM Medium 151 Source stock batch input: 0.01 g. Retain provider-declared stock g_l=0.01 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| AlK(SO4)2 | 0.01 G_PER_L | Copied from referenced JCM Medium 151 Source stock batch input: 0.01 g. Retain provider-declared stock g_l=0.01 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| H3BO3 | 0.01 G_PER_L | [Merged 2 duplicates: 0.015, 0.01] | Copied from JCM Medium 151 Source stock batch input: 0.01 g. Retain provider-declared stock g_l=0.01 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Na2MoO4 x 2 H2O | 0.01 G_PER_L | Copied from referenced JCM Medium 151 Source stock batch input: 0.01 g. Retain provider-declared stock g_l=0.01 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Distilled water | 1 L | Source water batch input: 1000 ml = 1 L. This is not a measured final volume. Prior duplicate-collapse notes describe the superseded flattened record. |
| KOH solution | Source-explicit stock pH adjustment; no fixed dose. |
Source stock addition: 10 ml = 0.01 L per receiving batch, not grams per liter or a dose per final liter.
Sodium sulfide separately prepared solution
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2S x 9 H2O | Copied from referenced JCM Medium 151 Source batch input: 0.5 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. This is the contribution specified for the receiving medium batch, not the mass for preparing an unspecified volume of stock. |
The source's 5% label has no specified percentage basis or stock addition volume. Neither is inferred. Composition notes describe the receiving-batch contribution, not an unspecified stock-batch recipe.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.111631Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J261 |
| 2026-02-03T11:26:54.326470 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:19:14.577797Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002619 |
| 2026-03-17T19:45:27.859671+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "JCM MEDIUM No. 261" to "jcm_medium_no_261" for consistent matching |
| 2026-04-04T18:49:19.093618 | copy_referenced_compositions | RESOLVED_REFERENCE | Resolved reference by copying from pyrococcus_medium (CultureMech:002510) |
| 2026-05-16T08:57:36.300213+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(2 keys) -> list(1 entries) |
| 2026-06-05T07:41:10.033632+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.261022+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-08T14:34:18.972918+00:00 | codex | RESTORED_JCM261_VARIANT_FORMULATION_WITH_EVIDENCE | Source-defined variant overrides and reciprocal links restored. Parent formulations and prior history preserved. Batch amounts and stock composition are not final-medium concentrations. Unknown percentage basis/dose remains unresolved; chemical annotations preserved, not recertified. |