Overview
- External ontology
- mediadive.medium:J153 (source link unavailable) — LEEMING AND NOTMAN AGAR
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=153
Composition
flowchart LR
CultureMech_002512["leeming_and_notman_agar"]
ing0_Bacto_peptone["Bacto peptone"]
CultureMech_002512 --> ing0_Bacto_peptone
ing1_Glucose["Glucose"]
CultureMech_002512 --> ing1_Glucose
CHEBI_17234["CHEBI:17234"]
ing1_Glucose -.-> CHEBI_17234
ing2_Yeast_extract["Yeast extract"]
CultureMech_002512 --> ing2_Yeast_extract
ing3_Oxgall["Oxgall"]
CultureMech_002512 --> ing3_Oxgall
ing4_Glycerol["Glycerol"]
CultureMech_002512 --> ing4_Glycerol
CHEBI_17754["CHEBI:17754"]
ing4_Glycerol -.-> CHEBI_17754
ing5_Glycerol_monostearate["Glycerol monostearate"]
CultureMech_002512 --> ing5_Glycerol_monostearate
CHEBI_75456["CHEBI:75456"]
ing5_Glycerol_monostearate -.-> CHEBI_75456
ing6_Tween_60["Tween 60"]
CultureMech_002512 --> ing6_Tween_60
CHEBI_53425["CHEBI:53425"]
ing6_Tween_60 -.-> CHEBI_53425
ing7_Cow_s_milk["Cow's milk"]
CultureMech_002512 --> ing7_Cow_s_milk
ing8_Agar["Agar"]
CultureMech_002512 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto peptone | 9.88142 G_PER_L | ||
| 2 |
Glucose
C6H12O6 180.156 g/mol
|
4.94071 G_PER_L |
CHEBI:17234
glucose
|
|
| 3 | Yeast extract | 0.0988142 G_PER_L | ||
| 4 | Oxgall | 7.90514 G_PER_L | ||
| 5 |
Glycerol
C3H8O3 92.094 g/mol
|
1 G_PER_L |
CHEBI:17754
glycerol
|
|
| 6 |
Glycerol monostearate
C21H42O4 358.563 g/mol
|
0.494071 G_PER_L |
CHEBI:75456
2-stearoylglycerol
|
|
| 7 |
Tween 60
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H46O6 606.838 g/mol
|
0.5 G_PER_L |
CHEBI:53425
polysorbate 60
|
|
| 8 | Cow's milk | 10 G_PER_L | ||
| 9 | Agar | 11.8577 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Autoclave at 110C for 15 min.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- leeming_and_notman_agar (CultureMech:002512)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.831938Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J153 |
| 2026-03-13T06:19:13.905454Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002512 |
| 2026-03-17T06:30:07.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.079469+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LEEMING AND NOTMAN AGAR" to "leeming_and_notman_agar" for consistent matching |
| 2026-06-05T07:41:11.050837+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:40.161935+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 33424fefd3be01b798b2adb03b0673f9ef2465275082cb88ca934094d4921e6a |