leeming_and_notman_agar

CultureMech:002512 bacterial COMPLEX SOLID_AGAR
Original name: LEEMING AND NOTMAN AGAR

Overview

External ontology
mediadive.medium:J153 (source link unavailable) — LEEMING AND NOTMAN AGAR
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=153

Composition

flowchart LR
    CultureMech_002512["leeming_and_notman_agar"]
    ing0_Bacto_peptone["Bacto peptone"]
    CultureMech_002512 --> ing0_Bacto_peptone
    ing1_Glucose["Glucose"]
    CultureMech_002512 --> ing1_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing1_Glucose -.-> CHEBI_17234
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_002512 --> ing2_Yeast_extract
    ing3_Oxgall["Oxgall"]
    CultureMech_002512 --> ing3_Oxgall
    ing4_Glycerol["Glycerol"]
    CultureMech_002512 --> ing4_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing4_Glycerol -.-> CHEBI_17754
    ing5_Glycerol_monostearate["Glycerol monostearate"]
    CultureMech_002512 --> ing5_Glycerol_monostearate
    CHEBI_75456["CHEBI:75456"]
    ing5_Glycerol_monostearate -.-> CHEBI_75456
    ing6_Tween_60["Tween 60"]
    CultureMech_002512 --> ing6_Tween_60
    CHEBI_53425["CHEBI:53425"]
    ing6_Tween_60 -.-> CHEBI_53425
    ing7_Cow_s_milk["Cow's milk"]
    CultureMech_002512 --> ing7_Cow_s_milk
    ing8_Agar["Agar"]
    CultureMech_002512 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Bacto peptone 9.88142 G_PER_L
2 Glucose
C6H12O6 180.156 g/mol
4.94071 G_PER_L CHEBI:17234
glucose
3 Yeast extract 0.0988142 G_PER_L
4 Oxgall 7.90514 G_PER_L
5 Glycerol
C3H8O3 92.094 g/mol
1 G_PER_L CHEBI:17754
glycerol
6 Glycerol monostearate
C21H42O4 358.563 g/mol
0.494071 G_PER_L CHEBI:75456
2-stearoylglycerol
7 Tween 60
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H46O6 606.838 g/mol
0.5 G_PER_L CHEBI:53425
polysorbate 60
8 Cow's milk 10 G_PER_L
9 Agar 11.8577 G_PER_L CHEBI:2509
agar

Preparation

  1. Autoclave at 110C for 15 min.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.831938Z mediadive-import Imported from MediaDive Source: JCM, ID: J153
2026-03-13T06:19:13.905454Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002512
2026-03-17T06:30:07.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:27.079469+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LEEMING AND NOTMAN AGAR" to "leeming_and_notman_agar" for consistent matching
2026-06-05T07:41:11.050837+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-10-07T14:33:27.670564+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.