Overview
- External ontology
- mediadive.medium:J170 (source link unavailable) — ARTIFICIAL DEEP LAKE VITAMIN MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.4
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=170
Composition
flowchart LR
CultureMech_002530["artificial_deep_lake_vitamin_medium"]
ing0_NaCl["NaCl"]
CultureMech_002530 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002530 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002530 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_KCl["KCl"]
CultureMech_002530 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002530 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Sodium_succinate["Sodium succinate"]
CultureMech_002530 --> ing5_Sodium_succinate
CHEBI_63675["CHEBI:63675"]
ing5_Sodium_succinate -.-> CHEBI_63675
ing6_Yeast_extract["Yeast extract"]
CultureMech_002530 --> ing6_Yeast_extract
ing7_Biotin["Biotin"]
CultureMech_002530 --> ing7_Biotin
CHEBI_15956["CHEBI:15956"]
ing7_Biotin -.-> CHEBI_15956
ing8_Vitamin_B12["Vitamin B12"]
CultureMech_002530 --> ing8_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing8_Vitamin_B12 -.-> CHEBI_176843
ing9_Thiamine_HCl["Thiamine HCl"]
CultureMech_002530 --> ing9_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing9_Thiamine_HCl -.-> CHEBI_49105
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
180 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
75 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
7.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
7.4 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
Sodium succinate
C4H4O4.2Na 162.052 g/mol
|
10 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
|
| 7 | Yeast extract | 1 G_PER_L | ||
| 8 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.1 G_PER_L |
CHEBI:15956
biotin
|
|
| 9 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 10 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.1 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Preparation
- Add components, except vitamin solution, to distilled water and bring volume to 990 ml. Adjust pH to 7.4. For preparation of solid medium, add 20.0 g/L Noble Agar (BD-Difco). Autoclave. After cooling to 50C, aseptically add the following vitamin solution (filter-sterilized):
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- artificial_deep_lake_vitamin_medium (CultureMech:002530)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.871201Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J170 |
| 2026-03-13T06:19:13.993083Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002530 |
| 2026-03-17T06:30:01.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.178852+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ARTIFICIAL DEEP LAKE VITAMIN MEDIUM" to "artificial_deep_lake_vitamin_medium" for consistent matching |
| 2026-06-05T07:40:49.720089+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:23.988071+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 83e173f719ac11b6d6d595a09638b2d14c8cd28498655edf0e8fdf54a863e543 |