artificial_deep_lake_vitamin_medium

CultureMech:002530 bacterial COMPLEX LIQUID
Original name: ARTIFICIAL DEEP LAKE VITAMIN MEDIUM

Overview

External ontology
mediadive.medium:J170 (source link unavailable) — ARTIFICIAL DEEP LAKE VITAMIN MEDIUM
Applications
  • Microbial cultivation
pH
7.4
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=170

Composition

flowchart LR
    CultureMech_002530["artificial_deep_lake_vitamin_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002530 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002530 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002530 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_KCl["KCl"]
    CultureMech_002530 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002530 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_Sodium_succinate["Sodium succinate"]
    CultureMech_002530 --> ing5_Sodium_succinate
    CHEBI_63675["CHEBI:63675"]
    ing5_Sodium_succinate -.-> CHEBI_63675
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_002530 --> ing6_Yeast_extract

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
180 G_PER_L CHEBI:26710
sodium chloride
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
75 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
7.4 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 KCl
Cl.K 74.551 g/mol
7.4 G_PER_L CHEBI:32588
potassium chloride
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 Sodium succinate
C4H4O4.2Na 162.052 g/mol
10 G_PER_L CHEBI:63675
sodium succinate (anhydrous)
7 Yeast extract 1 G_PER_L

Preparation

  1. Add components, except vitamin solution, to distilled water and bring volume to 990 ml. Adjust pH to 7.4. For preparation of solid medium, add 20.0 g/L Noble Agar (BD-Difco). Autoclave. After cooling to 50C, aseptically add the following vitamin solution (filter-sterilized):

Solutions

Vitamin solution

Dose: 10 ML_PER_L

Stock composition
IngredientConcentrationNotes
Biotin 0.1 G_PER_L
Vitamin B12 0.1 G_PER_L
Thiamine HCl 0.1 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.871201Z mediadive-import Imported from MediaDive Source: JCM, ID: J170
2026-03-13T06:19:13.993083Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002530
2026-03-17T06:30:01.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:27.178852+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ARTIFICIAL DEEP LAKE VITAMIN MEDIUM" to "artificial_deep_lake_vitamin_medium" for consistent matching
2026-06-05T07:40:49.720089+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-13T23:31:54.282053+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 3 stock-strength component(s) out of ingredients into 1 solution(s): 3 -> 'Vitamin solution' @ 10 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).