Overview
- External ontology
- mediadive.medium:J171 (source link unavailable) — SULFOLOBUS SOLFATARICUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 4.1
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=171
Composition
flowchart LR
CultureMech_002531["sulfolobus_solfataricus_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002531 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1__NH4_2SO4["(NH4)2SO4"]
CultureMech_002531 --> ing1__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing1__NH4_2SO4 -.-> CHEBI_62946
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002531 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002531 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002531 --> ing4_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing4_MnCl2_x_4_H2O -.-> CHEBI_86368
ing5_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
CultureMech_002531 --> ing5_Na2B4O7_x_10_H2O
CHEBI_131366["CHEBI:131366"]
ing5_Na2B4O7_x_10_H2O -.-> CHEBI_131366
ing6_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002531 --> ing6_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing6_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing7_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002531 --> ing7_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing7_CuCl2_x_2_H2O -.-> CHEBI_86318
ing8_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002531 --> ing8_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing8_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing9_VOSO4_x_n_H2O["VOSO4 x n H2O"]
CultureMech_002531 --> ing9_VOSO4_x_n_H2O
CHEBI_87020["CHEBI:87020"]
ing9_VOSO4_x_n_H2O -.-> CHEBI_87020
ing10_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002531 --> ing10_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing10_CoSO4_x_7_H2O -.-> CHEBI_91244
ing11_Yeast_extract["Yeast extract"]
CultureMech_002531 --> ing11_Yeast_extract
ing12_Casamino_acids["Casamino acids"]
CultureMech_002531 --> ing12_Casamino_acids
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
3.1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
2.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.25 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.0018 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 6 |
Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
0.0045 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
|
| 7 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.00022 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 8 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
5e-05 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 9 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
3e-05 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 10 |
VOSO4 x n H2O
(H2O)n.O5SV 181.021 g/mol
|
3e-05 G_PER_L |
CHEBI:87020
vanadyl sulfate hydrate
|
|
| 11 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
1e-05 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 12 | Yeast extract | 1 G_PER_L | ||
| 13 | Casamino acids | 1 G_PER_L |
Preparation
- Adjust pH to 4.0-4.2 with 10 N H2SO4.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- sulfolobus_solfataricus_medium (CultureMech:002531)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.873585Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J171 |
| 2026-01-28T19:48:41.794114Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:13.999321Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002531 |
| 2026-03-17T06:30:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.185962+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SULFOLOBUS SOLFATARICUS MEDIUM" to "sulfolobus_solfataricus_medium" for consistent matching |
| 2026-06-05T07:41:47.494716+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:50.688148+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:47:12.039140+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:32.830200+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 60eedb727003183c9e8da0008e3fefb4b4a699293b523a6559ce3d413d1f398e |