sulfolobus_solfataricus_medium

CultureMech:002531 archaea COMPLEX LIQUID
Original name: SULFOLOBUS SOLFATARICUS MEDIUM

Overview

External ontology
mediadive.medium:J171 (source link unavailable) — SULFOLOBUS SOLFATARICUS MEDIUM
Applications
  • Microbial cultivation
pH
4.1
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=171

Composition

flowchart LR
    CultureMech_002531["sulfolobus_solfataricus_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002531 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002531 --> ing1__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing1__NH4_2SO4 -.-> CHEBI_62946
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002531 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002531 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002531 --> ing4_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing4_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing5_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_002531 --> ing5_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing5_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing6_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002531 --> ing6_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing6_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing7_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002531 --> ing7_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing7_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing8_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002531 --> ing8_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing8_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing9_VOSO4_x_n_H2O["VOSO4 x n H2O"]
    CultureMech_002531 --> ing9_VOSO4_x_n_H2O
    CHEBI_87020["CHEBI:87020"]
    ing9_VOSO4_x_n_H2O -.-> CHEBI_87020
    ing10_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002531 --> ing10_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing10_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing11_Yeast_extract["Yeast extract"]
    CultureMech_002531 --> ing11_Yeast_extract
    ing12_Casamino_acids["Casamino acids"]
    CultureMech_002531 --> ing12_Casamino_acids

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
3.1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 (NH4)2SO4
2H4N.O4S 132.141 g/mol
2.5 G_PER_L CHEBI:62946
ammonium sulfate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.25 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.0018 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
6 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
0.0045 G_PER_L CHEBI:131366
disodium tetraborate decahydrate
7 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.00022 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
8 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
5e-05 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
9 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
3e-05 G_PER_L CHEBI:75213
sodium molybdate dihydrate
10 VOSO4 x n H2O
(H2O)n.O5SV 181.021 g/mol
3e-05 G_PER_L CHEBI:87020
vanadyl sulfate hydrate
11 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
1e-05 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
12 Yeast extract 1 G_PER_L
13 Casamino acids 1 G_PER_L

Preparation

  1. Adjust pH to 4.0-4.2 with 10 N H2SO4.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.873585Z mediadive-import Imported from MediaDive Source: JCM, ID: J171
2026-01-28T19:48:41.794114Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:13.999321Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002531
2026-03-17T06:30:54.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:27.185962+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SULFOLOBUS SOLFATARICUS MEDIUM" to "sulfolobus_solfataricus_medium" for consistent matching
2026-06-05T07:41:47.494716+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:50.688148+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:47:12.039140+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T14:33:27.670564+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.