Overview
- External ontology
- mediadive.medium:J173 (source link unavailable) — BARBOUR-SFOENNER-KELLY (BSK) II MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.6
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=173 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_002533["barbour_sfoenner_kelly_bsk_ii_medium"]
ing0_CMRL_1066["CMRL 1066"]
CultureMech_002533 --> ing0_CMRL_1066
ing1_Tryptone["Tryptone"]
CultureMech_002533 --> ing1_Tryptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_002533 --> ing2_Yeast_extract
ing3_Sodium_chloride["Sodium chloride"]
CultureMech_002533 --> ing3_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing3_Sodium_chloride -.-> CHEBI_26710
ing4_HEPES["HEPES"]
CultureMech_002533 --> ing4_HEPES
CHEBI_46756["CHEBI:46756"]
ing4_HEPES -.-> CHEBI_46756
ing5_Trisodium_citrate["Trisodium citrate"]
CultureMech_002533 --> ing5_Trisodium_citrate
CHEBI_53258["CHEBI:53258"]
ing5_Trisodium_citrate -.-> CHEBI_53258
ing6_Glucose["Glucose"]
CultureMech_002533 --> ing6_Glucose
CHEBI_17234["CHEBI:17234"]
ing6_Glucose -.-> CHEBI_17234
ing7_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_002533 --> ing7_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing7_Sodium_pyruvate -.-> CHEBI_50144
ing8_N_Acetyl_D_glucosamine["N-Acetyl-D-glucosamine"]
CultureMech_002533 --> ing8_N_Acetyl_D_glucosamine
CHEBI_506227["CHEBI:506227"]
ing8_N_Acetyl_D_glucosamine -.-> CHEBI_506227
ing9_NaHCO3["NaHCO3"]
CultureMech_002533 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
ing10_Yeast_extract["Yeast extract"]
CultureMech_002533 --> ing10_Yeast_extract
ing11_Neopeptone["Neopeptone"]
CultureMech_002533 --> ing11_Neopeptone
ing12_Gelatin["Gelatin"]
CultureMech_002533 --> ing12_Gelatin
CHEBI_5291["CHEBI:5291"]
ing12_Gelatin -.-> CHEBI_5291
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | CMRL 1066 | 9.9 G_PER_L | ||
| 2 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 3 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 4 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 5 | HEPES | 6 G_PER_L |
CHEBI:46756
HEPES
|
|
| 6 |
Trisodium citrate
C6H5O7.3Na 258.069 g/mol
|
0.7 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 7 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
|
| 8 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.8 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 9 |
N-Acetyl-D-glucosamine
C8H15NO6 221.209 g/mol
|
0.4 G_PER_L |
CHEBI:506227
N-acetyl-D-glucosamine
|
|
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 11 | Yeast extract | 2 G_PER_L | ||
| 12 | Neopeptone | 0.025 G_PER_L | ||
| 13 | Gelatin | 70 G_PER_L |
CHEBI:5291
gelatin
|
Preparation
- Adjust pH to 7.6 with 1 N NaOH and sterilize by filtration through a 0.22 �m filter (Millipore). Autoclave Solution B, then mix Solutions A and B, and add 40 ml of heat-inactivated (56C, 45 min) sterile rabbit serum (Pel-Freez Biologicals).
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- barbour_sfoenner_kelly_bsk_ii_medium (CultureMech:002533)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.878698Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J173 |
| 2026-03-13T06:19:14.011366Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002533 |
| 2026-03-17T06:30:03.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.199657+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BARBOUR-SFOENNER-KELLY (BSK) II MEDIUM" to "barbour_sfoenner_kelly_bsk_ii_medium" for consistent matching |
| 2026-06-05T07:40:50.494870+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:24.741279+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint d79c02ba96824279b4e43527b19fde1ea88f11f2e29794a427d59ff399799cd4 |